Method for simultaneously determining contents of 11 kinds of effective components in Reduning injection
A technology for active ingredients and injections, applied to the field of simultaneous determination of 11 ingredients in Reduning injection, which can solve the problems of rapid detection of unfavorable products and long running time
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Embodiment 1
[0069] a. Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, shanzhiside, and genipin Add 50% methanol to prepare appropriate amount of glycoside, genipin gentiobioside, geniposide, and deoxyloganin reference substances, the concentrations are 0.12225, 0.28953, 0.12357, 0.01606, 0.02849, 0.02323, 0.01670, 0.02468 , 0.23663, 0.40770, 0.05745 mg·ml -1 mixed reference solution.
[0070] b. Preparation of the test solution
[0071] Precisely draw 1ml of Reduning injection into a volumetric flask, dissolve it with 50% methanol and dilute to 100ml, shake well, centrifuge, and filter the supernatant through a 0.22um microporous membrane to obtain the product.
[0072] c. Determination method: adopt the UPLC-DAD method to accurately draw the reference substance solution and the test solution respectively, inject it into a liquid chromatograph, and meas...
Embodiment 2
[0077] a. Preparation of reference solution: Accurately weigh neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C, shanzhiside, and genipin glycoside, genipin gentiobioside, geniposide, deoxyloganin reference substance amount, add methanol to make the concentrations are 0.20001, 0.38333, 0.18445, 0.02661, 0.0408, 0.02865, 0.04000, 0.04845, 0.36654, 0.60076, 0.11475 mg·ml -1 mixed reference solution.
[0078] b. Preparation of the test solution
[0079] Precisely draw 1ml of Reduning injection into a volumetric flask, dissolve it with methanol and dilute to 100ml, shake well, centrifuge, and filter the supernatant through a 0.22um microporous membrane to obtain the product.
[0080] C, assay method: adopt UPLC-DAD method to accurately draw reference substance solution and need testing solution respectively, inject liquid chromatograph, measure, to obtain final product;
[0081] Chromatographic con...
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Abstract
Description
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Application Information
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