Method for detecting effective part group of periploca forrestii schltr

A technology of effective parts and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to control the quality of medicinal materials and their prescription preparations, qualitative and quantitative detection of medicinal active ingredients, and unseen content determination methods, etc.

Active Publication Date: 2019-04-19
贵州中医药大学
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Problems solved by technology

At present, Heiguteng still implements the 2003 edition of "Guizhou Province Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials Quality Standards". In this standard, there are only items such as source, character, microscopic identification and physical and chemical identification, and there is no qualitative and quantitative analysis of its medicinal ingredients. Testing, it is difficult to control the quality of medicinal materials and their prescriptions, and it is impossible to guarantee the safety and effectiveness of clinical medication
However, in recent years, most of the researches on S. chinensis have focused on the determination of chemical components, total saponins and total flavonoids, and pharmacological effects. Acid C and Proanthocyanidin A 2 Research Reports on Content Determination Methods
Limiting the further development and utilization of the Miao medicine Heiguteng

Method used

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  • Method for detecting effective part group of periploca forrestii schltr
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  • Method for detecting effective part group of periploca forrestii schltr

Examples

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Embodiment 1

[0076] A method for detecting the effective fractions of Rhizoma japonica; including detection of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid C and proanthocyanidin A 2 For content determination. The specific measurement method is:

[0077] Preparation of reference substance solution: Accurately weigh 5.02 mg of reference substances chlorogenic acid, 5.06 mg of cryptochlorogenic acid, 5.13 mg of neochlorogenic acid, 5.23 mg of isochlorogenic acid C and proanthocyanidin A 25.38 mg, placed in a 5 mL volumetric flask, dissolved, and shaken to make chlorogenic acid with a mass concentration of 1.004 mg / mL, cryptochlorogenic acid with a mass concentration of 1.012 mg / mL, and neochlorogenic acid with a mass concentration of 1.026 mg / mL mL, the mass concentration of isochlorogenic acid is 1.046 mg / mL and proanthocyanidin A 2 The reference substance solution whose mass concentration is 1.076 mg / mL;

[0078] Preparation of the test solution:

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Abstract

The invention discloses a method for detecting an effective part group of periploca forrestii schltr. The method is used for detecting the content of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid C and procyanidine A2, the detection method is accurate, the sensitivity is high, the repeatability is good, results are reliable, a basis is provided for quality control and evaluation of the periploca forrestii schltr medicinal material, and a foundation is laid for determining the optimal production place of the medicinal material. The method is beneficial for determining the appropriate growth area of the medicinal material on production, and is used for guiding medicinal material harvesting.

Description

technical field [0001] The invention relates to a method for detecting the effective part group of the vine vine, which belongs to the field of pharmaceutical technology. Background technique [0002] Blackbone Vine ( Periploca forrestii Schltr.) belongs to the family Asclepiadaceae (Asclepiadaceae) Periploca ) plant, recorded in the 2003 edition of "Quality Standards for Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials in Guizhou Province", has the functions of expelling wind and dampness, promoting blood circulation and dredging collaterals, eliminating numbness and relieving pain, and can relax tendons and collaterals, expelling wind and dehumidification; it is mainly used for rheumatoid arthritis, Bruises, stomach pain, indigestion, amenorrhea, dysentery, etc. [0003] Heiguteng is one of the commonly used Miao medicines, and it is an important component of Heiguteng Zhuifeng Huoluo Capsules, Heiguteng Shenjin Tougu Liquid, and Heiguteng Shen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 刘育辰刘刚杨洪秀张玲玲杨婉珠金文渊
Owner 贵州中医药大学
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