Method for detecting effective part group of periploca forrestii schltr
A technology of effective parts and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to control the quality of medicinal materials and their prescription preparations, qualitative and quantitative detection of medicinal active ingredients, and unseen content determination methods, etc.
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[0076] A method for detecting the effective fractions of Rhizoma japonica; including detection of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid C and proanthocyanidin A 2 For content determination. The specific measurement method is:
[0077] Preparation of reference substance solution: Accurately weigh 5.02 mg of reference substances chlorogenic acid, 5.06 mg of cryptochlorogenic acid, 5.13 mg of neochlorogenic acid, 5.23 mg of isochlorogenic acid C and proanthocyanidin A 25.38 mg, placed in a 5 mL volumetric flask, dissolved, and shaken to make chlorogenic acid with a mass concentration of 1.004 mg / mL, cryptochlorogenic acid with a mass concentration of 1.012 mg / mL, and neochlorogenic acid with a mass concentration of 1.026 mg / mL mL, the mass concentration of isochlorogenic acid is 1.046 mg / mL and proanthocyanidin A 2 The reference substance solution whose mass concentration is 1.076 mg / mL;
[0078] Preparation of the test solution:
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