A kind of detection method of the effective part group of Teng vine
A technology of effective parts and detection methods, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of qualitative and quantitative detection of active ingredients, unknown content determination methods, difficulty in controlling the quality of medicinal materials and their formulations, etc. Achieve the effect of accurate detection method, high sensitivity and reliable results
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[0076] A method for detecting the effective fractions of Rhizoma japonica; including detection of chlorogenic acid, cryptochlorogenic acid, neochlorogenic acid, isochlorogenic acid C and proanthocyanidin A 2 Carry out content determination. The specific measurement method is:
[0077] Preparation of reference solution: Accurately weigh 5.02 mg of reference substances chlorogenic acid, 5.06 mg of cryptochlorogenic acid, 5.13 mg of neochlorogenic acid, 5.23 mg of isochlorogenic acid C and proanthocyanidin A 25.38 mg, placed in a 5 mL volumetric flask, dissolved, and shaken to make chlorogenic acid with a mass concentration of 1.004 mg / mL, cryptochlorogenic acid with a mass concentration of 1.012 mg / mL, and neochlorogenic acid with a mass concentration of 1.026 mg / mL mL, the mass concentration of isochlorogenic acid is 1.046 mg / mL and proanthocyanidin A 2 The reference substance solution whose mass concentration is 1.076 mg / mL;
[0078] Preparation of the test solution:
[00...
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