Method for measuring content of active ingredients in Chinese honeylocust fruit

A technology of active ingredients and saponin, applied in the field of determination of traditional Chinese medicine ingredients, can solve the problems of quality evaluation, uneven quality of medicinal materials, etc., and achieve the effects of high accuracy, strong specificity and high sensitivity

Active Publication Date: 2022-06-28
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Saponaria has great medicinal value, but the quality of medicinal materi...

Method used

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  • Method for measuring content of active ingredients in Chinese honeylocust fruit
  • Method for measuring content of active ingredients in Chinese honeylocust fruit
  • Method for measuring content of active ingredients in Chinese honeylocust fruit

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preparation example Construction

[0038] By adopting the preparation method of the test solution of the present application, a test solution including 19 active ingredients can be obtained, so that the detection result of the active ingredient content in Saponin is more comprehensive, accurate and credible; wherein, through ultrasonic extraction, A test solution including 19 active ingredients is obtained, which is used to determine the content of each active ingredient, so that the detection result of the content of each active ingredient is more accurate.

[0039] In some embodiments of the present application, the chromatographic conditions include: chromatographic column: octadecylsilane bonded silica gel chromatographic column; mobile phase: phase A is formic acid aqueous solution with a volume fraction of 0.05-0.15%, and phase B is methanol ; Use volume fraction 5-90% phase A, 10-95% phase B, gradient elution; column temperature 20-30 °C; flow rate 0.1-0.5 mL / min; injection volume V 1 : 1-5 μL.

[0040]...

Embodiment 1

[0059] (1) Establish a standard curve for each active ingredient

[0060] Precisely weighed L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopolactone, licorice, apigenin, luteolin, eriodictyol, bergamot, luteolin, cryptochlorogen Acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin, isoorientin reference substance 10.0mg each, dissolved in methanol respectively, and prepared to a concentration of 1mg / mL The stock solution of each reference substance was stored in a refrigerator at 4°C for later use.

[0061] Accurately measure an appropriate amount of each reference substance stock solution, and prepare a mixed reference substance stock solution with methanol, marked as mark 1, where the concentration of L-malic acid is 50000ng / mL, the concentration of neochlorogenic acid is 16000ng / mL, and the concentration of cryptochlorogenic acid is 16000ng / mL. , The concentration of isovitexin is 8000ng / mL, the concentration ...

Embodiment 2-12

[0076] Except taking the saponin from batch S2 to batch S12 in Table 1 to replace the saponin in batch S1 in Example 1, the rest are the same as in Example 1, respectively obtaining the large soap from batch S2 to batch S12 The content of 19 active ingredients in the horn, the results are shown in Tables 4.1-4.2, wherein, the MRM diagram of the 19 active ingredients in the saponin of batch S10 is as follows Figure 1B shown.

[0077] Table 4.1 Contents of 19 active ingredients in different batches of Saponin (1) (μg / g)

[0078]

[0079] Table 4.2 Contents of 19 active ingredients in different batches of Saponin (2) (μg / g)

[0080]

[0081]

[0082] Methodological validation

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Abstract

The invention provides a method for determining the content of active ingredients in Chinese honeylocust fruit, which adopts ultra-high performance liquid chromatography-mass spectrometry. The content of L-malic acid, protocatechuic aldehyde, protocatechuic acid, caffeic acid, scopoletin, liquiritigenin, apigenin, luteolin, eriodictyol, citrus aurantium, fustin, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, vitexin, isovitexin, quercitrin, orientin and isoorientin in the Chinese honeylocust fruit can be rapidly detected. The method provided by the invention has the advantages of simple operation, high sensitivity, fast analysis speed and strong specificity, and can be used for quality control of Chinese honeylocust fruit.

Description

technical field [0001] The application relates to the technical field of determination of traditional Chinese medicine ingredients, and in particular, to a method for determining the content of active ingredients in Saponin. Background technique [0002] Gleditsiae sinensis fructus, also known as acacia, flat saponin, is a plant of the genus Gleditsiae sinensis, and is one of the unique native tree species in China. It is distributed in most parts of the country and has abundant resources. It has the effects of dispelling wind and phlegm and removing dampness and toxin. After processing, it can reduce its toxic and side effects, and is often used in clinical medicine. Saponin has great medicinal value, but the quality of medicinal materials in different origins is uneven, so it is difficult to evaluate its quality. Therefore, an efficient, rapid, and highly sensitive detection method is required to determine the content of active ingredients in Saponin, and to provide a bas...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8679G01N2030/065Y02A50/30
Inventor 何俊杨冬月欧阳慧子常艳旭高秀梅
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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