Preparation method of neochlorogenic acid

A new chlorogenic acid, extraction method technology, applied in the field of chemistry, can solve problems such as shortening time

Active Publication Date: 2015-11-18
JIANGSU KANION PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its advantages are as follows: a. In terms of process, this experiment is completed by three steps of extraction, enrichment and purification, which does not involve repeated silica gel column chromatography, avoids the use of a large amount of organic solvents, reduces pollution, reduces costs, and shortens time; b In terms of preparation am...

Method used

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  • Preparation method of neochlorogenic acid
  • Preparation method of neochlorogenic acid
  • Preparation method of neochlorogenic acid

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[0041] The invention discloses a preparation method of a new chlorogenic acid, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0042] The raw materials and reagents used in the preparation method of the new chlorogenic acid provided by the present invention can be purchased from the market. Among them, the Reveleris ...

Embodiment 1

[0045] 1. Extraction process

[0046] Take 10.0kg of honeysuckle, reflux extraction with 10 times the amount of 70% ethanol twice (2h / time), filter, and concentrate the filtrate under reduced pressure to a relative density of 1.05g·mL -1 (25°C). Stir and add ethanol until the volume fraction of ethanol is 80%, let it stand overnight, filter, concentrate the filtrate under reduced pressure until it has no alcohol smell, add water to dilute to 1:1, let stand overnight, filter, discard the insoluble matter, and get the filtrate for later use.

[0047] 2. Screening of resin models

[0048] Select 5g of each of the 4 pretreated resins (HPD200A, D101, HP20, AB-8) and put them in a 100mL conical flask with a stopper, add the honeysuckle extract concentrate into the bottle, let it stand at room temperature for 24h, after the adsorption balance, filter Remove the resin, quantitatively pipette the supernatant, measure the content of new chlorogenic acid, and calculate the adsorption...

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Abstract

The invention relates to the chemical field, in particular to a preparation method of neochlorogenic acid. According to the preparation method, Flos Lonicerae japonicae is subjected to reflux extraction through ethanol, filtered, concentrated, separated with macroporous adsorption resin and subjected to gradient elution through ethanol and water; elution parts of neochlorogenic acid are collected after HPLC (high performance liquid chromatography) analysis and detection; neochlorogenic acid is prepared quickly with a medium-low pressure preparative chromatograph and is above a gram level; meanwhile, neochlorogenic acid is subjected to structural identification and purity detection, and the purity can be as high as 98.86%. Neochlorogenic acid prepared with the method meets requirements of reference substances for content detection, and a new method for preparing high-purity neochlorogenic acid on a large scale is provided.

Description

technical field [0001] The invention relates to the field of chemistry, in particular to a preparation method of new chlorogenic acid. Background technique [0002] Neochlorogenic acid is a product extracted and refined from the dried flower buds of Lonicera japonica Thunb. (Lonicera japonica Thunb.), which is a monocaffeoylquinic acid compound and one of the main organic acid components in honeysuckle. Caffeoylquinic acid compounds are a kind of phenolic acid natural compounds formed by condensing quinic acid and different numbers of caffeic acid through esterification reaction. These compounds widely exist in plants and have significant antioxidant activity. , Antimicrobial effect, enzyme inhibition, liver cell protection, inhibition of platelet aggregation, etc. Caffeoylquinic acid compounds are widely distributed in plants, and have a variety of specific pharmacological activities. It is believed that with the deepening and development of research on caffeoylquinic acid...

Claims

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Application Information

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IPC IPC(8): C07C69/732C07C67/48C07C67/56
CPCC07C67/48C07C67/56C07C69/732
Inventor 萧伟倪付勇刘露宋亚玲赵祎武黄文哲王振中周习
Owner JIANGSU KANION PHARMA CO LTD
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