Method for extracting solanesol and chlorogenic acid from discard tobacco leaves

A technology of waste tobacco leaves and solanesol, which is applied in the field of simultaneously extracting solanesol and chlorogenic acid, and extracting solanesol and chlorogenic acid, can solve the problems of reduced yield, easy oxidation of chlorogenic acid, and increased production cost. Achieve the effects of increasing income, improving comprehensive utilization efficiency, and simplifying the production process

Active Publication Date: 2013-07-17
CHINA TOBACCO HUNAN INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at the same time, the inventor also found that chlorogenic acid is easy to oxidize and solanesol is easy to decompose during the extraction process. If the separation is not handled properly, it will not only reduce the yield of the two active ingredients, but also increase the overall production cost.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) 100 kg of waste tobacco leaves with a water content of 7.5% were pulverized and mixed with 500 L of 95% ethanol solution for extraction, and then refluxed at 38°C for 4 hours and then filtered through a ceramic membrane once; (2) the step (1 ) the obtained extraction residue is mixed with ethanol solution under the same conditions for extraction, the ceramic membrane is filtered once, and the two extracts are combined; (3) the extract obtained in step (2) is concentrated under reduced pressure at 40°C to obtain a dry product 25.8kg; (4) Dissolve the dry matter with 60L of water, filter it once with a ceramic membrane to obtain a filtrate, dissolve the filter residue with 60L of water, and filter it once with a ceramic membrane to obtain a filtrate, combine the two filtrates and carry out at 40°C Concentrate under reduced pressure to 30L; (5) Dissolve 16.5kg of filter residue with 35L of ethyl acetate, filter it once with a ceramic membrane to remove insoluble matter,...

Embodiment 2

[0051] (1) 200 kg of waste tobacco leaves with a moisture content of 6.8%, were crushed and then mixed with 800 L of 95% ethanol solution for extraction, reflux extraction at 38°C for 4 hours, and then filtered once with a ceramic membrane; (2) Step (1) ) the obtained extraction residue is mixed with ethanol solution under the same conditions for extraction, the ceramic membrane is filtered once, and the two extracts are combined; (3) the extract obtained in step (2) is concentrated under reduced pressure at 40°C to obtain a dry product 63.65kg; (4) Dissolve the dry matter with 150L of water, filter it once with a ceramic membrane to obtain a filtrate, dissolve the filter residue with 150L of water, and filter it once with a ceramic membrane to obtain a filtrate, combine the two filtrates and carry out at 40°C Concentrate under reduced pressure to 70L; (5) Dissolve 34.8kg of filter residue with 80L of ethyl acetate, filter it once with a ceramic membrane to remove insoluble mat...

Embodiment 3

[0053](1) 500kg of waste tobacco leaves with a water content of 8.4% were pulverized and mixed with 1800L of 95% ethanol solution for extraction, reflux extraction at 38°C for 4 hours and then filtered once with a ceramic membrane; (2) Step (1) ) the obtained extraction residue is mixed with ethanol solution under the same conditions for extraction, the ceramic membrane is filtered once, and the two extracts are combined; (3) the extract obtained in step (2) is concentrated under reduced pressure at 40°C to obtain a dry product 143.5kg; (4) Dissolve the dry matter with 300L of water, filter it once with a ceramic membrane to obtain a filtrate, dissolve the filter residue with 300L of water, and filter it once with a ceramic membrane to obtain a filtrate. Concentrate under reduced pressure to 100L; (5) Dissolve 81.2kg of filter residue with 180L of ethyl acetate, filter it once with a ceramic membrane to remove insoluble matter, put it on a silica gel column for chromatographic ...

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Abstract

The invention provides a method for extracting solanesol and chlorogenic acid from discard tobacco leaves. The method comprises the following steps: (1) subjecting the discard tobacco leaves to reflux extraction with an aqueous ethanol solution with a concentration of 90 to 97% at a temperature of 35 to 40 DEG C and then filtering an extract with a ceramic membrane; (2) subjecting the extract to reduced pressure condensation at a temperature of 38 to 42 DEG C so as to obtain a dried substance; (3) dissolving the dried substance with water, carrying out filtration with the ceramic membrane, subjecting a filtrate to reduced pressure condensation so as to obtain a concentrate and standing a filter residue for subsequent usage; (4) dissolving the filter residue with ethyl acetate, carrying out filtration with the ceramic membrane, carrying out chromatographic separation by using a silica gel column, carrying out elution with absolute ethyl alcohol and subjecting an eluate to condensation and then to crystallization so as to obtain solanesol; and (5) carrying out chromatographic separation on the concentrate obtained in step (3) by using a macro-porous resin, carrying out elution with an aqueous ethanol solution with a concentration of 55 to 65%, condensing an eluate, adjusting a pH value to 3 to 3.5 and carrying out crystallization so as to obtain chlorogenic acid. The method provided by the invention has a simple process, is convenient to operate, effectively improves the utilization rate of the discard tobacco leaves, reduces production cost for chlorogenic acid and solanesol, has high yield and can effectively guarantee large scale production.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting solanesol and chlorogenic acid, and more specifically relates to a method for simultaneously extracting solanesol and chlorogenic acid from waste tobacco leaves. Background technique [0002] Tobacco (Nicotiana tobacum L) is an annual or perennial plant of the Solanaceae Nicotiana family, with alternate single leaves and often sticky pilose, native to South America. Our country introduced tobacco very early, and the tobacco grown like other countries is mainly used as cigarettes. At present, my country is one of the largest tobacco-producing countries in the world. Its planting area and output rank first in the world. The planting area is about 1.3 million hectares, and the annual output is 4.5-5 million tons, of which nearly 25% are leftovers such as tobacco leaves and tobacco powder. Obsolete and cannot be used in cigarette production. The main p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C33/02C07C29/76C07C69/732C07C67/48
Inventor 范胜兴
Owner CHINA TOBACCO HUNAN INDAL CORP
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