Process of extracting and separating ursolic acid from plant

A technology for ursolic acid and plants, which is applied to the field of extracting and separating ursolic acid from plants, can solve the problems of large loss of components, incomplete solvent removal, and increased production cost, and achieves low cost, easy availability of raw materials, and high extraction efficiency. Effect

Inactive Publication Date: 2003-11-05
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to experiments, these solvents are incomplete in removing impurities, and re

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Embodiment 1: according to technical scheme of the present invention, extract the ursolic acid in the Paulownia leaf, carry out according to the following steps: 1. extract

[0012] Take the coarse powder of paulownia leaves, add 6-8 times the amount of methanol to reflux extraction, the first two times are two hours each, and the last two times are one hour each. Combine the extracts and let stand overnight. After filtration, the filtrate was concentrated, methanol was recovered to a small volume, and the precipitate was placed. Filter, discard the filtrate, and wash the precipitate with a small amount of acetone several times to remove impurities. After dissolving in absolute ethanol, it was heated and filtered, and placed for crystallization. Recrystallize once in the same way. Finally, it was recrystallized with methanol-ethanol (1:5) mixed solvent. Stand to get white needle crystals. 2. Identification

[0013] Take a small amount of this crystal and dissolve ...

Embodiment 2

[0015] Embodiment 2: The difference between this embodiment and embodiment 1 is that in step 1, absolute ethanol is used for reflux extraction.

Embodiment 3

[0016] Embodiment 3: The difference between this embodiment and embodiment 1 is that in step 1, Soxhlet extraction is used and petroleum ether and chloroform are used to remove impurities.

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Abstract

The present invention is process of extracting and separating ursolic acid from plant. The extracting and separating process of ursolic acid includes methanol reflux extraction of coarse paulownia leaf powder, recovering methanol to small volume, settling, filtering to eliminate filtrate, washing the precipitate with acetone for many times to eliminate impurity, and re-crystallization for three times in anhydrous alcohol and methanol to obtain ursolic acid. Compared with other processes of extracting ursolic acid, the present invention has the advantages of cheap material, simple technological process, low cost and being suitable for industrial production.

Description

[0001] 1. Field [0002] The invention belongs to the research of natural products, relates to the extraction of a natural product with important application value, in particular to a method for extracting and separating ursolic acid from plants. 2. Background technology [0003] Ursolic acid has obvious stabilizing and cooling effects. It is active against Gram-positive bacteria, negative bacteria and yeasts in vitro, and has a very significant inhibition rate on liver cancer cell culture in vitro. Animal experiments have proved that ursolic acid has the effect of reducing transaminase . The pharmaceutical industry has used ursolic acid as a raw material for pharmaceutical production. There are many research reports on ursolic acid. Many plants contain ursolic acid, but the content is different. The content of ursolic acid in Paulownia leaves is high, and this study was only reported in Japan in 1963. Regarding the extraction method of ursolic acid, most of the methods rep...

Claims

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Application Information

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IPC IPC(8): C07J63/00
Inventor 王军宪郭增军宋莉
Owner XI AN JIAOTONG UNIV
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