Production method of Lonicera japonica flower cells rich in chlorogenic acid substances

A production method, the technology of honeysuckle, which is applied in the biological field, can solve the problems of high price, short flowering period of honeysuckle, and large dosage

Inactive Publication Date: 2019-05-17
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, honeysuckle is the raw material of common beverages and traditional Chinese medicines in my country, and as an industrial raw material for extracting caffeoylquinic acid such as chlorogenic acid, the consumption is relatively large and the price is relatively high
Moreover, the flowering period of honeysuckle is short, the output is limited, and the scarce resources seriously restrict the development and utilization of chlorogenic acid.

Method used

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  • Production method of Lonicera japonica flower cells rich in chlorogenic acid substances
  • Production method of Lonicera japonica flower cells rich in chlorogenic acid substances
  • Production method of Lonicera japonica flower cells rich in chlorogenic acid substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0131] Example 1: Production of honeysuckle cells rich in chlorogenic acids

[0132] The implementation steps of this embodiment are as follows:

[0133] A. Pretreatment

[0134] The buds of honeysuckle were rinsed 4 times with clear water first, then soaked for 20 minutes in Diaopai washing powder solution with a concentration of 1.5% by weight, then rinsed with clear water, and dried;

[0135] B. Disinfection

[0136] The air-dried honeysuckle shoot that step A obtains is carried out disinfection treatment 25s in the alcohol solution of 70% by volume in concentration, then carries out disinfection treatment 10min in the sodium hypochlorite aqueous solution of 4% by weight in concentration;

[0137] The sterilized honeysuckle buds are rinsed with sterile water for 3 times, then placed on sterile filter paper to absorb moisture, and then under aseptic conditions, the honeysuckle buds are cut into cut honeysuckle buds with a size of 0.4cm;

[0138] C. Preparation medium

[...

Embodiment 2

[0154] Example 2: Production of honeysuckle cells rich in chlorogenic acids

[0155] The implementation steps of this embodiment are as follows:

[0156] A. Pretreatment

[0157] The buds of honeysuckle were first rinsed with clear water 5 times, then soaked for 15 minutes in Diaopai washing powder solution with a concentration of 2.5% by weight, then rinsed with clear water, and dried;

[0158] B. Disinfection

[0159] The air-dried honeysuckle bud that step A obtains is carried out disinfection treatment 30s in concentration in 65% alcohol solution by volume, then carries out disinfection treatment 12min in concentration in 5% sodium hypochlorite aqueous solution by weight;

[0160] The sterilized honeysuckle shoots are rinsed 5 times with sterile water, then placed on sterile filter paper to absorb moisture, and then under aseptic conditions, the honeysuckle shoots are cut into cut honeysuckle shoots with a size of 0.5 cm;

[0161] C. Preparation medium

[0162] (i) Callu...

Embodiment 3

[0177] Example 3: Production of honeysuckle cells rich in chlorogenic acids

[0178] The implementation steps of this embodiment are as follows:

[0179] A. Pretreatment

[0180] The honeysuckle buds were first rinsed with clear water for 6 times, then soaked for 18 minutes in Diaopai washing powder solution with a concentration of 2.0% by weight, then rinsed with clear water, and dried;

[0181] B. Disinfection

[0182] The air-dried honeysuckle tender bud that step A obtains is carried out disinfection treatment 28s in the alcohol solution of 75% by volume in concentration, then carries out disinfection treatment 8min in the sodium hypochlorite aqueous solution of 6% by weight in concentration;

[0183] The sterilized honeysuckle buds were rinsed 4 times with sterile water, then placed on sterile filter paper to absorb moisture, and then under aseptic conditions, the honeysuckle buds were cut into cut honeysuckle bud segments with a size of 0.6 cm;

[0184] C. Preparation...

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Abstract

The invention relates to a production method of Lonicera japonica flower cells rich in chlorogenic acid substances. The method comprises the following steps: pretreatment; disinfection; culture mediumpreparation; callus treatment; subculture; establishment of a Lonicera japonica flower cell suspension culture system and posttreatment. Content of the chlorogenic acid substances in the product produced with the method is as high as 9.68% or higher which is increased by 100%-100.83% as compared with the content 4.82%-4.84% of chlorogenic acid of natural Lonicera japonica flower buds on the basisof the conventional production method (Determination of eight components in Lonicerae japonicae flos by HPLC [J]. Chinese Traditional and Herbal Drugs, 2014, 45(7):1006-1009 by Limiao, Wangyongxiangand Mengjin), production period is shortened by 300-330 days, and cost is reduced by 70%-80% through cost accounting. Indusial and automatic production is easy to realize, and the method can be widelypromoted and used.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology. More specifically, the present invention relates to a method for producing honeysuckle cells rich in chlorogenic acids. 【Background technique】 [0002] Since the beginning of the 21st century, health has become the subject of people's pursuit. People advocate nature, return to nature, use existing natural resources to maintain food safety, prevent and treat diseases, and restore the body, pursuing the natural health of life, which leads to the high efficiency of plant functional ingredients Produced research boom. [0003] Chlorogenic acid, also known as caffeoylquinic acid (Cafeoylquinic acid), is an important plant secondary metabolite, a family of phenylpropanoid lipid compounds formed by the shikimic acid pathway in the process of plant body aerobic respiration, quinine Esterification of acid is divided into 3-O-caffeoylquinic acid (chlorogenic acid), 4-O-caffeoylquinic acid (cryptochlorogeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 熊建华李冬梅杜娟杜华英胡昭君罗秋水
Owner JIANGXI AGRICULTURAL UNIVERSITY
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