Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof

A technology of extract and Gewang, which is applied in the field of medicine, can solve the problems of failing to give full play to Gewang, not clarifying the active ingredients of Gewang extract, and not being clear about the material basis of medicinal effects

Inactive Publication Date: 2012-02-08
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Gewang is widely used clinically, but the substance basis of its efficacy is not clear. Due to the variety of active ingredients in traditional Chinese medicine, the existing technology research has not clarified the active ingredients of Gewang extract, which has failed to give full play to the good application of Gewang.

Method used

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  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof
  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof
  • Wikstroemia indica (L.) C.A.Mey extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 The preparation of Gewang extract

[0039] Gewang extract was prepared as follows:

[0040] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, concentrating the extract under reduced pressure at 40°C and drying it to obtain 80 g of the extract;

[0041] (2) Extract 80 g of the extract three times with 150 mL of petroleum ether, 150 mL of chloroform, 150 mL of ethyl acetate, and 150 mL of n-butanol, regardless of the order of extraction, or use one or two or more extraction solvents to extract separately;

[0042] (3) Get ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol (100: 1-1: 1, volume ratio) gradient elution; Collect chloroform: methanol: water volume ratio is 50:1 to 1:1 eluted components, to obtain the effective part; the effecti...

Embodiment 2

[0056] Embodiment 2 The preparation of Gewang extract

[0057] Gewang extract was prepared as follows:

[0058] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, the extract was concentrated at 40° C. under reduced pressure and dried to obtain 85 g of extract;

[0059] (2) Extract 80g of the extract with 300mL of petroleum ether, 300mL of chloroform, 300mL of ethyl acetate, and 300mL of n-butanol, respectively, for 4 times. extraction;

[0060] (3) Take ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol: water (100: 1: 0-1: 1: 1, volume ratio) gradient elution; Collect chloroform: Methanol: water volume ratio is 100: 1 to 50: 1 elution component, obtains effective part; Effective part continues through chloroform: methanol (100: 1-1: 1, volume ratio) gradi...

Embodiment 3

[0064] Embodiment 3 The preparation of Gewang extract

[0065] Gewang extract was prepared as follows:

[0066] (1) After taking 1000 g of the aerial part of Gewang and pulverizing it (about 1 cm in length), adding 8 L of ethanol with a concentration of 95% by volume and extracting at room temperature for 2 hours, concentrating the extract under reduced pressure at 40°C and drying it to obtain 80 g of the extract;

[0067] (2) Extract 80g of the extract with 500mL of petroleum ether, 500mL of chloroform, 500mL of ethyl acetate, and 500mL of n-butanol, respectively, for 4 times. extraction;

[0068] (3) Take ethyl acetate extract or n-butanol extract and carry out silica gel column chromatography, through chloroform: methanol: water (100: 1: 0-1: 1: 1, volume ratio) gradient elution; Collect chloroform: Methanol: the eluting fraction of water volume ratio is 50:1 to 1:1, obtains effective fraction; Effective fraction continues to pass through chloroform:methanol (30:1-1:1, vo...

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Abstract

The invention discloses a wikstroemia indica (L.) C.A.Mey extract as well as a preparation method and application thereof. The wikstroemia indica (L.) C.A.Mey extract has the effective compositions of: naringenin, 5, 6, 7- trihydroxy-4'-methoxyflavanone, p-hydroxybenzoic acid, benzoic acid, quercetin glycoside,genkwano1 B, daphnoretin, Lirioresinol, Sikokianin and the like. The invention simultaneously provides application of the wikstroemia indica (L.) C.A.Mey extract, in particular the favorable application in preparing anti-cancer or anti-inflammation analgesia drug. A cytotoxicity experiment shows that the wikstroemia indica (L.) C.A.Mey extract disclosed by the invention has cytotoxicity functions on breast cancer of human, breast cancer drugresistant cell strain, mouse breast adenocarcinoma, which are in linear relation.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a Ligewang extract and its preparation method and application. Background technique [0002] Liaogewang (Wikstroemia indlca (L.) C.A.Mey), commonly used Chinese herbal medicine among the people, is bitter and cold in nature and flavor, slightly pungent, poisonous, and is mainly used for bruises, dispelling blood stasis and relieving pain. Staphylococcus aureus is highly sensitive and moderately sensitive to Pseudomonas aeruginosa and Salmonella typhi; the water decoction of rhizome bark has obvious inhibitory effect on Staphylococcus aureus in test tube; the ethyl acetate soluble part of root bark ethanol extract It also has antibacterial effect in vitro, and the flavonoid glycosides contained in it have inhibitory effects on Staphylococcus aureus, hemolytic streptococcus, pneumococcus, and Escherichia coli in preliminary experiments; The minimum inhibitory concentr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/20C07D311/40C07D311/30C07D311/32C07C63/06C07C65/03C07C51/42A61K31/353A61K31/352A61K31/192A61P29/00A61P35/00A61P15/14
Inventor 沈志滨尹永芹崔红花张鑫
Owner GUANGDONG PHARMA UNIV
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