A comprehensive control method for black rot disease in cauliflower containing biological agents.

Abstract

This invention discloses a comprehensive control method for black rot disease in cauliflower containing a biological agent, comprising the following steps: S1, transplanting treatment: 200-500 ml of the mixed biological agent is applied to the roots of transplanted cauliflower seedlings as a root drench; the mixed biological agent contains at least 1 × 10⁻⁶ viable bacteria. 8 S1. A bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a CFU / mL ratio of 1-3:1; S2. Spraying treatment: After transplanting cauliflower seedlings, spray the plants with a compound microbial agent; the compound microbial agent contains a bacterial suspension of *Penicillium restrictum* and *Paecilomyces lilacinus* at a volume ratio of 4-6:1; the viable count of both *Penicillium restrictum* and *Paecilomyces lilacinus* bacterial suspensions is not less than 1×10⁻⁶. 8 CFU / mL. The method described in this invention can effectively reduce the incidence of black rot in cauliflower during the transplanting to survival period, survival to heading period, and heading to harvest period.

Description

Technical Field

[0001] This invention belongs to the field of vegetable disease control technology, specifically, it relates to a comprehensive control method for black rot disease of cauliflower containing biological agents. Background Technology

[0002] Cauliflower (Brassica oleracea var. botrytis L.), also known as cauliflower or florets, is a variety of Brassica oleracea in the Brassicaceae family, cultivated for its florets. As one of the world's most important vegetable varieties, cauliflower is rich in nutrients, tender in texture, and delicious in flavor. It is also high in water content and low in calories, making it a filling and healthy choice that is increasingly popular with consumers.

[0003] Cauliflower is increasingly popular in the market, leading to a growing planting area. However, this increase in planting area has also resulted in a rising incidence of black rot. Black rot is a bacterial disease caused by *Xanthomonas brassicae* subsp. *brevicornu*. It can occur during the transplanting, heading, and seed-producing stages of cauliflower, with a long disease cycle. Infection during the transplanting and heading stages, in particular, can cause plant deformities, hinder growth and development, and even lead to death, posing a significant threat. Summary of the Invention

[0004] In order to overcome the problems existing in the prior art, this invention proposes a comprehensive control method for black rot disease of cauliflower containing biological agents.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solution:

[0006] A comprehensive control method for black rot disease in cauliflower containing biological agents includes the following steps:

[0007] S1, Transplanting Treatment: Apply a mixed microbial agent to the roots of transplanted cauliflower seedlings, using 200-500 ml per seedling; the mixed microbial agent contains at least 1 × 10⁻⁶ viable bacteria. 8 CFU / mL of localized penicillin ( Penicillium restrictum ), pale purple Paecilomyces ( Paecilomyces lilacinus The bacterial suspensions of the two bacteria were in a volume ratio of 1-3:1.

[0008] S2, Spraying treatment: After transplanting, cauliflower seedlings are treated by spraying them with a compound microbial agent; the compound microbial agent contains a suspension of Penicillium localization and Paecilomyces lilacinus at a volume ratio of 4-6:1; the viable count of both Penicillium localization and Paecilomyces lilacinus suspensions is not less than 1×10⁻⁶. 8 CFU / mL.

[0009] Further, the method for preparing the Penicillium spp. suspension is as follows: the Penicillium spp. test tube strain is inoculated into PDB liquid culture medium and cultured at 26℃-28℃ and 180-200r / min to obtain the liquid strain; the PDB liquid culture medium contains: 200g potato, 20g grape, 1000ml water, and the pH of the culture medium is 6.0-6.5.

[0010] Further, the method for preparing the Paecilomyces lilacinus suspension is as follows: Paecilomyces lilacinus test tube strain is inoculated into PDB liquid fermentation medium and cultured at 25-28℃ and 180-200 r / min to obtain a liquid strain; the PDB liquid fermentation medium contains 200g potato, 20g glucose, 3g KH2PO4, 1.5g MgSO4·7H2O, 8mg VitanibB1 Trace, and 1L water, with the medium at its natural pH.

[0011] Through the above technical solution, the present invention can achieve at least the following beneficial effects: the method described in the present invention can effectively reduce the incidence of black rot in cauliflower during the transplanting to survival period, survival to heading period, and heading to harvesting period. Detailed Implementation

[0012] Unless otherwise stated, all materials and reagents used in this invention are commercially available.

[0013] The cauliflower seeds used in this invention are "Shengsong 718" cauliflower seeds from Tianjin Gengyun Seed Industry Co., Ltd.

[0014] This invention is limited to Penicillium ( Penicillium restrictum Purchased from Beijing Bio-Bio Biotechnology Co., Ltd., strain number: Bio-09873.

[0015] In this invention, the pale purple Paecilomyces ( Paecilomyces lilacinus Purchased from Beijing Bio-Bio Biotechnology Co., Ltd., strain number: Bio-52225.

[0016] The method for preparing Penicillium spp. suspension is as follows: the purchased Penicillium spp. test tube culture is inoculated into PDB liquid medium (200 g potato, 20 g grape, 1000 ml water; pH=6.0-6.5), and cultured at 26℃-28℃, 180-200 r / min to obtain liquid culture.

[0017] The preparation method of Paecilomyces lilacinus suspension is as follows: Paecilomyces lilacinus test tube inoculum is inoculated into PDB liquid fermentation medium (200g potato, 20g glucose, 3g KH2PO4, 1.5g MgSO4·7H2O, 8mg Vitanib B1 (thiamine) Trace, 1L water, natural pH), and cultured at 25-28℃ and 180-200r / min to obtain liquid inoculum. Example 1

[0018] A comprehensive control method for black rot disease in cauliflower containing biological agents includes the following steps:

[0019] S1, Transplanting Treatment: Apply the mixed microbial agent to the roots of transplanted cauliflower seedlings, using 300ml per seedling; the mixed microbial agent contains approximately 1×10⁻⁶ viable bacteria. 8 CFU / mL of localized penicillin ( Penicillium restrictum ), pale purple Paecilomyces ( Paecilomyces lilacinus The bacterial suspensions of the two were in a volume ratio of 2:1.

[0020] S2, Spraying Treatment: After transplanting cauliflower seedlings, spray the plants with a compound microbial agent. Spray every 10 days from transplant survival to head formation, then every 7 days from the start of head formation, ending 7 days before harvest. The compound microbial agent contains a suspension of *Penicillium spp.* and *Paecilomyces lilacinus* at a volume ratio of 5:1; the viable count of both suspensions is approximately 1 × 10⁻⁶. 8 CFU / mL. Example 2

[0021] A comprehensive control method for black rot disease in cauliflower containing biological agents includes the following steps:

[0022] S1, Transplanting Treatment: Apply a mixed microbial agent to the roots of transplanted cauliflower seedlings, 200 ml per cauliflower plant; the mixed microbial agent contains approximately 1 × 10⁻⁶ viable bacteria. 8 CFU / mL of localized penicillin ( Penicillium restrictum ), pale purple Paecilomyces ( Paecilomyces lilacinus The bacterial suspensions of the two were in a 1:1 volume ratio.

[0023] S2, Spraying Treatment: After transplanting, cauliflower seedlings are established and treated with a compound microbial agent. Spray every 10 days from transplant establishment to head formation, then every 7 days from head formation onwards, ending 7 days before harvest. The compound microbial agent contains a suspension of *Penicillium spp.* and *Paecilomyces lilacinus* at a volume ratio of 4:1. The viable count of both *Penicillium spp.* and *Paecilomyces lilacinus* suspensions is approximately 1 × 10⁻⁶. 8 CFU / mL. Example 3

[0024] A comprehensive control method for black rot disease in cauliflower containing biological agents includes the following steps:

[0025] S1, Transplanting Treatment: Apply the mixed microbial agent to the roots of transplanted cauliflower seedlings, using 500ml per seedling; the mixed microbial agent contains approximately 1×10⁻⁶ viable bacteria. 8 CFU / mL of localized penicillin ( Penicillium restrictum ), pale purple Paecilomyces ( Paecilomyces lilacinus The bacterial suspensions of the two were in a volume ratio of 3:1.

[0026] S2, Spraying Treatment: After transplanting, cauliflower seedlings are treated with a compound microbial agent by spraying the plants. From transplant survival to head formation, spray once every 10 days; from the beginning of head formation, spray once every 7 days, ending 7 days before harvest. The compound microbial agent contains a suspension of *Penicillium spp.* and *Paecilomyces lilacinus* at a volume ratio of 6:1; the viable count of both *Penicillium spp.* and *Paecilomyces lilacinus* suspensions is approximately 1 × 10⁻⁶. 8 CFU / mL.

[0027] The difference between Comparative Example 1 and Example 1 is that in the mixed bacterial agent, the Penicillium localization bacterial suspension was replaced with a Paleopyramidus suspension.

[0028] The difference between Comparative Example 2 and Example 1 is that the suspension of Paecilomyces lilacinus in the mixed bacterial agent was replaced with a suspension of Penicillium spp.

[0029] The difference between Comparative Example 3 and Example 1 is that in the compound microbial agent, the Penicillium spp. suspension was replaced with a Paleopyramidus suspension.

[0030] The difference between Comparative Example 4 and Example 1 is that the bacterial suspension of Paecilomyces lilacinus in the compound bacterial agent is replaced with a bacterial suspension of Penicillium spp.

[0031] The experiment included two control groups: Control Group 1 and Control Group 2.

[0032] The difference between Control Group 1 and Example 1 is that only step S1 was performed, and the mixed bacterial agent was replaced with the same volume of sterile water.

[0033] The difference between Control Group 2 and Example 1 is that in step S2, the compound bacterial agent is replaced with the same volume of sterile water.

[0034] During the experiment:

[0035] In each embodiment, comparative example, and control group 1 and 2, 200 cauliflower plants with uniform growth and no obvious diseases were selected for transplanting and root drenching treatment. The root drenching treatment included applying a mixed microbial agent to the roots of the cauliflower plants after transplanting, followed by a second root drenching with an effective viable bacterial count of 1×10⁻⁶ days later.8 200 ml of a suspension of *Xanthomonas brassicae* subsp. *brachiatum* (CFU / mL) was applied to the roots. The incidence of black rot in cauliflower plants was recorded 7 days after root drenching with the pathogen, and this incidence was recorded as the black rot incidence rate from transplanting to the survival stage.

[0036] After statistically analyzing the incidence of black rot in transplanted cauliflower plants during the survival period, 100 cauliflower plants with uniform growth and no obvious disease were selected for step S2. The compound microbial agent was sprayed according to the recorded technical plan, and the leaves and stems of the plants were sprayed thoroughly.

[0037] The period from the completion of the statistics on the incidence of black rot from transplanting to the survival period until the first cauliflower plant forms a head is considered the survival to head-forming period.

[0038] After the incidence of black rot from the seedling stage to the heading stage was statistically analyzed, 50 cauliflower plants with uniform growth and no obvious disease were selected and sprayed with compound microbial agent according to the recorded technical plan. During the spraying, the front and back of the plant leaves, stems and flower heads were sprayed in place.

[0039] The period from the first cauliflower head to maturity and harvest is called the head-forming and harvesting period.

[0040] Disease incidence rate = (Number of diseased plants / Total number of plants) * 100%

[0041] The statistical results are shown in Table 1 below:

[0042] Table 1. Statistics on the incidence of black rot in cauliflower

[0043] deal with Black rot incidence rate during transplanting to survival period / % Black rot incidence rate from survival to bulb formation stage / % Black rot incidence rate from heading to harvest / % Example 1 3 5 4 Example 2 4 7 6 Example 3 2 3 2 Comparative Example 1 55.5 6 6 Comparative Example 2 67.5 4 4 Comparative Example 3 3.5 17 14 Comparative Example 4 3 24 20 Control Group 1 97.5 / / Control Group 2 4 33 32

[0044] As shown in Table 1 above, drenching cauliflower plants with a mixed microbial agent containing Penicillium localum and Paecilomyces lilacinus, and spraying cauliflower plants with a compound microbial agent containing Penicillium localum and Paecilomyces lilacinus, can significantly reduce the incidence of black rot in cauliflower plants during the transplanting to survival period, survival to heading period, and heading to harvesting period.

[0045] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.

Claims

1. A comprehensive control method for black rot disease in cauliflower containing biological agents, characterized in that: Includes the following steps: S1, Transplanting Treatment: Apply a mixed microbial agent to the roots of transplanted cauliflower seedlings, using 200-500 ml per seedling; the mixed microbial agent contains at least 1 × 10⁻⁶ viable bacteria. 8 CFU / mL of localized penicillin ( Penicillium restrictum ), pale purple Paecilomyces ( Paecilomyces lilacinus The bacterial suspensions of the two bacteria were in a volume ratio of 1-3:

1. S2, Spraying treatment: After transplanting, cauliflower seedlings are treated by spraying them with a compound microbial agent; the compound microbial agent contains a suspension of Penicillium localization and Paecilomyces lilacinus at a volume ratio of 4-6:1; the viable count of both Penicillium localization and Paecilomyces lilacinus suspensions is not less than 1×10⁻⁶. 8 CFU / mL; The pathogen causing black rot in cauliflower is Xanthomonas brassicae subsp. Brassicae.

2. The integrated control method for cauliflower black rot containing biological agents according to claim 1, characterized in that: The method for preparing the Penicillium spp. suspension is as follows: Penicillium spp. test tube culture is inoculated into PDB liquid culture medium and cultured at 26℃-28℃ and 180-200r / min to obtain the liquid culture; the PDB liquid culture medium contains: 200g potato, 20g grape, 1000ml water, and the pH of the culture medium is 6.0-6.

5.

3. The integrated control method for cauliflower black rot containing biological agents according to claim 1, characterized in that: The method for preparing the Paecilomyces lilacinus suspension is as follows: Paecilomyces lilacinus test tube culture is inoculated into PDB liquid fermentation medium and cultured at 25-28℃ and 180-200 r / min to obtain the liquid culture. The PDB liquid fermentation medium contains 200g potato, 20g glucose, 3g KH2PO4, 1.5g MgSO4·7H2O, 8mg Vitanib B1 Trace, and 1L water, with the medium at its natural pH.