Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 125 in blood and preparation method thereof

Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 125 in blood and a preparation method thereof. A coating film, a magnet particle pad combined with tumor associated antigen 125 antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A tumor associated antigen 125 antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology and the fluorescein isothiocyanate system into the quantitative detection of tumor associated antigen 125, and has the advantages that the detection sensitivity is greatly improved, the accurate quantitative results can be given, the operation is simple and convenient and the diagnosis is rapid.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting tumor-associated antigen 125 in blood and a preparation method thereof. Background technique [0002] Tumor-associated antigen 125 (CA125) has a molecular weight of 220,000 to 1,000,000, and the sugar content only accounts for 24% of the total protein. CA125 is prepared from mouse anti-human papillary cystic ovarian epithelial cell line OC125. An elevated serum CA125 level is a reliable indicator of tumor presence and recurrence. CA125 can be significantly increased in 80-90% of ovarian epithelial cancers, and the CA125 content can also be significantly increased in patients with endometrial cancer, clear cell carcinoma, fallopian tube cancer and undifferentiated ovarian cancer; the positive rate of mucinous ovarian cancer patients is Low, CA125 is particularly valuable in the differentiation of benign and m...

AI Technical Summary

Problems solved by technology

However, it should be pointed out that the sensitivity of diagnosing recurrence with elevated serum CA125 levels is lower than the specificity, that is, elevated serum CA125 levels indicate recurrence to a large extent, but normal CA125 neither indicates the presence of tumors nor the absence of tumors progress

[0003] The current diagnostic techniques for CA125 in blood mainly include: radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), chemiluminescence immunoassay (CLIA), time-resolved fluorescence immunoassay, colloidal gold immunochromatography and other methods, but these Each method has its own characteristics and shortcomings: radioimmunoassay is a commonly used method in clinical practice. Its advantages are relatively accurate results and a wide linear range. Environmental pollution has been gradually replaced by other methods

ELISA is similar to chemiluminescence and time-resolved fluorescence method in principle, but there is a difference in sensitivity due to the difference in the final detection system. At present, automation, large-scale, and quantitative detection have been realized, but there are large differences in results between methods, and the linear range is relatively small. At the same time, automated testing is currently monopolized by large foreign manufacturers, and the equipment is expensive, and it is not suitable for single-person and small-batch testing, which greatly limits their application in primary hospitals and clinics.

In recent years, colloidal gold immunochromatography has appeared to detect CA125, which is fast, convenient, and single-serve operation, but the disadvantage is that it can only achieve semi-quantitative, can only indicate a rough range, and cannot achieve accurate quantification, and abnormal samples need to use other methods Recheck testing increases the cost of medical treatment for the testees, and it is difficult to promote in the market, so it is difficult to be widely used

Images