Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 15-3 in blood and preparation method thereof

CN101762697AInactive Publication Date: 2010-06-30CHEMCLIN DIAGNOSTICS CO LTD
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  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 15-3 in blood and preparation method thereof
  • Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 15-3 in blood and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation method of magnetic immunochromatography test strip and test paper box for quantitative detection of CA15-3 in blood

[0030] The preparation method of test strip and test paper box of the present embodiment comprises the following steps:

[0031] A. Antibody preparation: commercialized CA15-3 paired antibody was selected, dialyzed against 20mM, pH7.2 (pH7.0-7.6 are applicable) PBS at 4°C overnight for future use.

[0032] B. Preparation of coating film:

[0033] Preparation of coating buffer: 0.02M phosphate buffer (PB) with pH 7.2 was used as coating buffer, sterilized by 0.22 μm microporous membrane filter and stored at 4°C for later use, valid for two weeks.

[0034] Preparation of blocking solution: 0.02M phosphate buffered saline (PBS) containing 0.5% BSA, pH 7.2 (pH 7.0-7.6 is applicable), 0.22μm microporous membrane filter sterilization, and store at 4°C for later use , valid for one week.

[0035] Preparation of coating membrane: Dilute the CA15-3...

Embodiment 2

[0062] In addition to the step of preparing the magnetic particles of the CA15-3 antibody: the ratio of the CA15-3 antibody to the magnetic particle is 1:3 for the CA15-3 antibody, and the other steps are the same as in Example 1.

Embodiment 3

[0064] Except for the step of preparing the magnetic particles of the CA15-3 antibody: the ratio of the CA15-3 antibody to the magnetic particles was 1:8, and other steps were the same as in Example 1.

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Abstract

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 15-3 in blood and a preparation method thereof. A coating film, a magnet particle pad combined with tumor associated antigen 15-3 antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A tumor associated antigen 15-3 antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology into the quantitative detection of tumor associated antigen 15-3, and has the advantages that the detection sensitivity is greatly improved, the accurate quantitative results can be given, the operation is simple and convenient and the diagnosis is rapid.

Description

technical field [0001] The invention belongs to the field of medical testing, in particular to a magnetic immunochromatographic test strip for quantitatively detecting tumor-associated antigen 15-3 in blood and a preparation method thereof. Background technique [0002] In 1984, Hilkens et al. made a mouse monoclonal antibody (115-DB) from the glycoprotein MAM-6 on the human milk fat globule membrane. In 1984, Kufu et al. made a monoclonal antibody (DF-3) from the liver metastatic breast cancer cell membrane. CA15-3 is a breast cancer-associated antigen, a glycoprotein with a molecular weight of more than 400kD. Patients with breast cancer often have elevated CA15-3, but the sensitivity is low in the early stage of breast cancer, and the positive rate of metastatic breast cancer can reach 80%. CA15-3 can be used as an auxiliary diagnostic indicator for primary breast cancer, and can also be used as an indicator for follow-up after surgery to monitor tumor recurrence and met...

Claims

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Application Information

Patent Timeline
30 Jun 2010
Publication
CN101762697A
IPC
G01N33/574; G01N33/531; G01N33/532; G01N33/577; G01N33/558
Inventors
唐宝军; 应希堂