96 results about "Biotin Metabolism" patented technology

Antimicrobial peptides enable an alternate approach to developing antimicrobial coatings due to their targeting of the membranes of the bacteria. High specific activity is achieved by orienting the peptides so that the antimicrobial ends of the peptides maximally contact the bacteria. In one embodiment, one end of the peptide is covalently attached directly to the substrate. In another embodiment, the peptides are immobilized on the substrate using a coupling agent or tether. Non-covalent methods include coating the peptide onto the substrate or physiochemically immobilizing the peptides on the substrate using highly specific interactions, such as the biotin / avidin or streptavidin system. The compositions are substantially non-leaching, antifouling, and non-hemolytic. The immobilized peptides retain sufficient flexibility and mobility to interact with and de endocytosed by the bacteria, viruses, and / or fungi upon exposure. Immobilizing the peptides to the substrate reduces concerns regarding toxicity of the peptides and the development of antimicrobial resistance, while presenting substantially all of the peptide at the site of action at the surface of the substrate.

The present invention is directed to methodology that allows a variety of compounds to be attached to self-assembling peptides using biotin / streptavidin linkages. The peptides can be used to form a biologically compatible membrane that promotes the growth and differentiation of cells. The attached therapeutic agents can be used to promote this process and the gel along with the growing cells can be implanted at a site in vivo where tissue repair is needed. Alternatively, membranes can be used for culturing cells in vitro or can be used for delivering drugs in vivo in the absence of seeded cells.

The methods and compositions disclosed herein concern the synthesis of a novel class of “two-headed” phospholipid-phosphoinositide hybrids possessing a carbon backbone, such as 2,3-diacylthreitol, erythritol or a synthetic module. The second phospholipid head group allows introduction of a biochemical or chemical moiety in a position orthogonal in space to those occupied by the phosphoinositide head group and the two acyl chains. The diacyl moieties allow for the incorporation of Pea-PIP2 into a lipid bilayer, while the Ptdlns(4,5)P2 moiety in the aqueous layer is specifically recognized by lipid binding proteins. In alternative embodiments of the invention, reporters, for example biotin, fluorophores and / or spin labels, are attached to the free amino group of the head groups of such molecules to specifically target the reporters to the lipid-water interface.

The invention provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ biotin ligase mutants and biotin analogs recognized by such mutants.

The invention discloses a chromatographic detection kit based on an aptamer, as well as a preparation method and detection method of the kit. The test paper of the kit includes a bottom board, and a sample pad, a bonding pad, a cellulose nitrate film and a water absorption pad, which are adhered on the bottom board and overlap one another; a detection line is arranged on the side of the cellulose nitrate film close to the bonding pad, and a quality control line is arranged on the side of the cellulose nitrate film close to the water absorption pad; an A aptamer labeled by colloidal gold is applied on the bonding pad; a composite containing a B aptamer and streptavidin is applied on the detection line; streptavidin is applied on the quality control line; and both of the A aptamer and the B aptamer are aptamers labeled by biotin and capable of specifically identifying a same test object. The chromatographic detection kit can display the result in five minutes only by directly dropping a diluted sample to be tested into a sample hole, is simple, quick, sensitive, easy to read result, and convenient and simple to prepare, and so on, and has the advantages of simplicity in operation and high specificity without using antibodies and instruments.

Methods of preparing targeting ligand bound avidin-lipid vesicles for use in preparing a targeted, therapeutic liposome composition are disclosed. Each vesicle comprises an avidin molecule coupled to the polymer-conjugated biotin which retains multiple free site biotin-binding sites such that the vesicle may be used to further couple a biotinylated-targeting ligand.

The preparation of vitamin D compounds of formula (I) with a label attached to a spacer group in the 3 position is disclosed.In the above formula (I), O represents the oxygen atom of an ether group; Y represents hydrogen or hydroxy; A represents a label such as biotin, digoxigenin, or another vitamin D group; R represents a substituted hydrocarbon side-group of vitamin D or a vitamin D metabolite. Also disclosed is a method of measuring 25-hydroxy vitamin D metabolite and a 1α,25-dihydroxy vitamin D metabolite in a sample.

The preparation of vitamin D compounds of formula (I) with a label attached to a spacer group in the 3 position is disclosed. In the above formula (I), O represents the oxygen atom of an ether group; Y represents hydrogen or hydroxy; A represents a label such as biotin, digoxigenin, or another vitamin D group; R represents a substitured hydrocarbon side-group of vitamin D or a vitamin D metabolite. Also disclosed is a method of measuring 25-hydroxy vitamin D metabolite and a 1α,25-dihydroxy vitamin D metabolite in a sample.

The invention discloses a content analysis and detection method of twelve compound vitamins for injection. Liposoluble components including vitamin A palmitate, vitamin D3 and racemic alpha-tocopherol are detected under the same high-performance liquid phase method conditions, a test solution is prepared by extracting a preparation content by non-polar organic solvent, and the detection wavelength is 265+ / -3nm. Water-soluble components including cocarboxylase tetrahydrate, riboflavin sodium phosphate, vitamin B6, vitamin C, nicotinamide, folic acid and dexpanthenol are detected under the same high-performance liquid phase method conditions, and the detection wavelength is 210+ / -3nm. Biotin and vitamin B12 are subjected to high-performance liquid phase method based detection, the detection wavelength is 200-600nm, and the preferable detection wavelength for the vitamin B12 is 550+ / -3nm. The method has high applicability.

The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.

The invention relates to a magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and a preparation method thereof. The test strip is assembled by pasting a coated film, magnetic particles combined with an AFP antibody, a sample pad and a water-absorbing pad which are mutually staggered by 2 millimeters in turn on a bottom plate and then covering an upper layer with a transparent plastic sealing film, wherein the coated film is precoated with an AFP-antibody detection line and a quality control line. As the test strip introduces a magnetic immunochromatography technique and a biotin-avidin system into the quantitative detection of AFP in blood, the test strip has the advantages of greatly increasing detection sensitivity, providing accurate quantitative results, reducing the labor intensity of operators and ensuring that clinicians can quickly diagnose patients.

The invention relates to a colloidal gold immunochromatography test strip used for testing an H1N1 influenza antigen and a method for testing the H1N1 influenza antigen. The test strip comprises a base plate, a sample pad, a conjugate pad, a pyroxylin film and an absorbent paper, wherein the sample pad, the conjugate pad, the pyroxylin film and the absorbent paper are lapped and adhered on the base plate sequentially; the conjugate pad is a combination of a gold-label-biotin conjugate pad and a streptavidin-gold-antibody conjugate pad; according to the method for testing the H1N1 influenza antigen, a sample to be tested is dropwise added on the test strip; if a red band is displayed at a testing line (1), the sample to be tested contains the H1N1 influenza antigen. According to the advantages, the test strip provided by the invention is cheap in price, fast, simple, convenient, and greatly suitable for on-site testing; moreover, the test strip can meet the requirement of higher testing sensitivity, and has an important clinical diagnostic significance.

The invention discloses a photoelectrochemical biosensor for detecting 5-hydroxymethylcytosine (5hmC) deoxyribonucleotide and a preparation method thereof. The photoelectrochemical biosensor comprisesan electrode and tungsten sulfide, polydopamine, mercaptobenzoic acid, 5hmC, phos-tag-biotin and streptavidin which are sequentially modified on the surface of the electrode. High sensitivity and high specificity detection of 5 hmC can be realized by use of the good photoelectric activity and biocompatibility of the tungsten sulfide, the excellent electronic donor properties and biocompatibilityof the polydopamine, the specific recognition and binding properties of phos-tag-biotin to phosphoric acid groups, and the specific reaction of hydroxymethyl and sulfydryl groups on the 5hmC catalyzedby M.HhaI methyltransferase. The method can well eliminate the interference of 5mC to the detection of the 5hmC. The detection method is simple, instrument miniaturization is realized, the photoelectrochemical biosensor is easy to operate, and the detection of the 5hmC can be realized by simple treating of the surface of the electrode.

A method for treating dyslipidemia and / or post prandial hyperglycemia by administering a combination of a chromium complex and biotin to an individual in need thereof is disclosed. The two compounds are administered orally or parenterally in daily dosages which provide between 25 μg and 1,000 μg of chromium and between 25 μg and 20 mg biotin. A method for reducing the glycemic index of food is similarly provided.

A pharmaceutical preparation comprising progestin, estrogen and a multivitamin agent. The progestin may be between 0 and 0.50 mg Desogesterel and the estrogen may be between 0 and 0.2 mg Ethinyl Estradiol. The multivitamin agent may be any combination of alpha carotene, beta carotene, biotin, bioflavonoid, calcium, chasteberry fruit, chromium, copper, coenzyme Q10, cryptoxanthin, dong quai root, folic acid, ginkgo biloba, garlic, grape seed extract, green tea extract, hesperedin, iodine, iron, lutein, malic acid, manganese, magnesium, milk thistle, molybdenum, niacin, panthothenic acid, potassium, pyridoxine HCL, quercetin, riboflavin, rutan, selenium, thiamine, vitamin A, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K, zinc, zoexanthin and any combination thereof.

This invention provides a method for detecting genetically modified organism (GMO) comprising amplifying transgenes of GMO by biotin-labeled primer sets, hybridizing the amplified products with colored bead-labeled probes, and detecting the hybrids. This invention also provides a polynucleotide for detecting a transgene of genetically modified soybean. This invention further provides a kit for detecting genetically modified soybean comprising biotin-labeled primer sets and colored bead-labeled probes.

The invention discloses fluorescent immunochromatography test paper and a preparation method thereof. The test paper is formed by sequentially overlapping a sample pad (1), an antibody carrying film (2) and absorbent paper (3) through a base plate (4) which is provided with binding agents, the antibody carrying film (2) is coated with a marker mixture joint line (5), a testing line (6) and a quality control line (7) in sequence from the end close to the sample pad (1), and the marker mixture joint line (5) is formed by arranging a marker mixture obtained by coupling fluorescent microspheres with an antibody 1 and biotin within a pretreatment area (8) which is pretreated with the antibody carrying film in a coating mode through a crossed film metal-spraying instrument. By means of the test paper, the problems that the result is not accurate caused by insufficient release amount due to the fact that excessive binding materials remain on glass fibers are solved, the tested fluorescence value is raised, and the sensitivity or detection upper limit is lifted. Meanwhile, in the production, the antibody carrying film is coated with the marker mixture obtained by coupling the fluorescent microspheres with the antibody 1 and the biotin, the marker mixture joint line, the T line and the C line can be coated simultaneously, time and processes are saved, and the production efficiency is improved.

The invention discloses a fluorescent immunochromatographic detection card, and a preparation method and application thereof. The fluorescent immunochromatographic detection card comprises a treating fluid A, a treating fluid B and a detection card, wherein the treating fluid A contains an antibody 1 of a coupling fluorescent microsphere for an antigen to be detected; the treating fluid B contains an antibody 2 of a coupling biotin for the antigen to be detected; the detection card comprises a detection line area and a quality control line area; the detection line area is fixed with a streptavidin detection T line; the quality control line area is fixed with an antibody quality control C line. The preparation method comprises: (1) preparing the treating fluid A; (2) preparing the treating fluid B; (3) drawing lines on the detection card. The fluorescent immunochromatographic detection card has the characteristics of high sensitivity, high specificity, high stability and the like, and can be applied to quick detection of a disease mark.

A composition is provided which contains policosanol and biotin and which may be used for treating, preventing and or reducing metabolic syndrome, hypercholesterolemia and hypoglycemia related diseases, total cholesterol, LDL-cholesterol, LDL / HDL ratio, triglycerides, coronary heart disease (heart attacks and strokes), inflammation, deep-vein thrombosis, immunoregulatory diseases, cardiovascular diseases, obesity, insulin resistance, dyslipidemia, raised blood pressure, fatigue, premenstrual syndrome, anxiety, depression and / or neurodegenerative disorders, and / or raising HDL cholesterol in humans and animals. The method comprises administering policosanol and biotin which together effectively lower blood glucose levels and lower the LDL / HDL cholesterol ratio. Typically, the administered composition includes about 0.1-10:1 parts by weight of policosanol to biotin.

The invention discloses a biosensor, a preparation method and a target molecule concentration detection method thereof. The preparation method for the biosensor comprises the following steps: (S1) modifying one or more of streptavidin, carboxyl, amino and sulfydryl onto a magnetic bead and modifying biotin onto a nucleic acid aptamer of a to-be-detected target molecule; (S2) adding the nucleic acid aptamer and the magnetic bead into a reaction solution and coupling the nucleic acid aptamer onto a local site of the magnetic bead, wherein the reaction solution is a salt buffer solution in concentration above 100mM; (S3) coupling the DNA sequence capable of complementarily pairing with the nucleic acid aptamer onto a noble metal nanoparticle; and (S4) reacting the magnetic bead acquired in the step S2 and the noble metal nanoparticle acquired in the step S3 for 5-60min in a hybridization solution, thereby forming the biosensor by the magnetic bead, nucleic acid aptamer and noble metal nanoparticle under the effect of base complementary pairing. The biosensor is capable of realizing high-sensitivity concentration detection, is low in cost and is high in efficiency.

The present invention is a natural formulation for treatment of male, female and adolescent pattern hair loss. The formulation contains a combination of Green Tea leaf extract, Polyphenols, Epigallocatechin Gallate (EGCG), Vitamin E, Folic Acid, Copper (as Amino Acid Chelate), B12, Zinc (as Oxide), Calcium Pantothenate, Niacin, Biotin, Riboflavin, Thiamine, and optionally Inositol, Black Tea Extract and Nettle Extract. The various extracts are prepared according to traditional procedures, and then combined in a suitable formulation for administration to the patient for treatment of male, female and adolescent pattern hair loss.

The invention relates to a magnetic immuno-chromatographic test paper strip for quantitatively detecting carcinoembryonic antigen in blood and a preparation method thereof. A coating film, a magnet particle pad combined with CEA antibodies, a sample pad and a water absorbing pad are sequentially and alternately stuck on a base plate in a staggering way at intervals of 2mm, and then the upper layer is covered by a transparent plastic sealing film to form a test paper strip. A CEA antibody detection line and a quality control line are pre-coated on the coating film. The invention introduces the magnetic immuno-chromatographic technology and the biotin-aviden system into the quantitative detection of CEA in blood, and has the advantages that the detection sensitivity is greatly improved, the accurate quantitative results can be given, the labor strength of the operators is reduced, and the clinicians can rapidly and accurately diagnose the illness of patients.

In this invention, anti-PEG antibodies or anti-methoxyl-PEG (anti-CH3O-PEG) antibodies were expressed on cell surface which can collocate with a biotinylated anti-PEG antibody (AGP4-Biotin) or biotinylated PEG (PEG-Biotin) to develop a cell-based sandwich ELISA or a cell-based competition ELISA, respectively. Both of these two methods could sensitively quantify free PEG and PEG-modified macromolecules (proteins, nanoparticles and liposomes) as sensitive as nano-gram level.

The invention provides a method for preparing a D-(+)-biotin intermediate. The method comprises the steps of: taking L-cysteine monohydrochloride as a starting material; using benzaldehyde and sodium cyanate as a ring closure reagent to synthesize (7aR)-3-phenyl-6-benzyl-1H, 3H-imidazo[1, 5-C]thiazole-(6H, 7aH)-5, 7-dione through the cyclization; then utilizing benzyl bromide to perform benzyl protection on N atoms; then taking zinc as a reducing agent to perform ring-opening synthesis on N, N-dibenzyl-L-sulfhydryl hydantoin; introducing a side chain through an esterification reaction with monomethyl adipate acyl chloride; and taking titanium as the reducing agent to perform reductive ring closure to generate the intermediate. According to the method, the cheap and readily available sodium cyanate is used as the ring closure reagent to replace sodium isocyanate which is toxic and difficult to purchase in an original method, reaction conditions are optimized and reaction order is adjusted, so the disadvantages of harsh reaction conditions and low yield in the original method of ring opening first and then benzyl protection are overcome by the method of performing benzyl protection on the N atoms of an imidazole part first and then performing ring opening; and the total yield reaches 34.0-38.0 percent.

An electrolyte for a lithium secondary battery, which includes a lithium salt, a nonaqueous organic solvent and at least one additive selected from the group consisting of vitamin G (vitamin B2, riboflavin), vitamin B3 (niacinamide), vitamin B4 (adenine), vitamin B5 (pantothenic acid), vitamin H (vitamin B7, biotin), vitamin M (vitamin B9, folic acid), vitamin BX (4-aminobenzoic acid), vitamin D2 (ergocalciferol), vitamin D3 (cholecalciferol), vitamin K1 (phylloquinone), ascorbyl palmitate, and sodium ascorbate.

A new detection method, for detecting a target substance using formation of an aggregate due to binding of a target substance and a binding substance that binds thereto, that is excellent in detection accuracy, and sensitivity, and a new detection reagent used for the same are provided. Modifying substances having a maximum diameter of about 50 nm or less bind to a binding substance that binds to a target substance, and a modified binding substance is prepared as a binding reagent. A target substance in a sample is detected by bringing this modified binding reagent into contact with the sample, and optically detecting an aggregate that is formed by binding of the modified binding substance and the target substance in the sample. Preferably, the modifying substance includes biotin or a biotin derivative and further includes avidin or an avidin derivative, and the avidin or the avidin derivative binds to the biotin or the biotin derivative. Further, preferably, the biotin or the biotin derivative binds to the binding substance via a spacer.

The invention relates generally to biotin-containing compounds that are useful as imaging agents and drug-delivery agents. Another aspect of the invention relates to the aforementioned compounds chelated to a metal atom. In a preferred embodiment, the metal atom is a gadolinium. Another aspect of the invention relates to a compound comprising three biotin moieties and a pharmaceutical agent covalently bound to a heterocyclic core. In certain embodiments, the pharmaceutical agent is an antibiotic, antiviral, or radionuclide. Another aspect of the present invention relates to a method of treating disease involving administering the compounds of the invention to a mammal. Another aspect of the present invention relates to a method of acquiring a magnetic resonance image using the compounds of the invention.

A process for reversible chemical modification of the luciferase, a process for the covalent conjugation of a reversibly modified luciferase to a chemical moiety (a protein or a binding partner such as biotin or an antibody), a process for reactivation of the reversibly-modified and inactivated luciferase, a process for making the said luciferase conjugates and a bioluminescent assay method that uses covalently conjugated firefly luciferase are taught. The present invention also relates to a composition comprising a reversibly modified luciferase, as well as a composition comprising a reversibly modified luciferase covalently conjugated to a chemical moiety.