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Fluorescent immunochromatography test paper and preparation method thereof

A technology of fluorescent immunochromatography and detection test paper, which is applied in the direction of measuring devices, analytical materials, instruments, etc., can solve the problems of poor uniformity of antibody-fluorescent conjugates, increased precision and accuracy, and retention of marker mixtures, etc., to achieve Effects of improving sensitivity or upper limit of detection, improving precision, and enhancing efficiency

Inactive Publication Date: 2015-12-23
XIAMEN BIOTIME BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] This method has three disadvantages: 1. Due to the material of the glass fiber membrane, only the mixture of fluorescent microspheres, antibody 1 and biotin-conjugated label can only be sprayed or soaked. The disadvantage of uniformity affects the precision and is not suitable for quantitative detection; if it is soaked, it will not only be uneven but also waste a lot of microspheres and antibodies, which will increase the cost; 2. The part of the marker mixture sprayed on the glass fiber membrane will be hooked The three-dimensional space of the glass fiber membrane leads to poor uniformity of each chromatography, and different degrees of marker mixtures remain on the glass fiber membrane each time, which increases the precision and accuracy
3. The uniformity of the spray film process is not as good as that of the coating process, resulting in poor uniformity of antibody-fluorescent conjugates

Method used

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  • Fluorescent immunochromatography test paper and preparation method thereof
  • Fluorescent immunochromatography test paper and preparation method thereof
  • Fluorescent immunochromatography test paper and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Embodiment 1: Preparation of fluorescent immunochromatography detection test paper

[0038] A fluorescent immunochromatographic detection test paper, which consists of a sample pad (1), an antibody-carrying film (2), and a water-absorbing paper (3) successively overlapping each other through a base plate (4) with an adhesive, wherein the The sample pad (1) is a glass cellulose film; the bottom plate with adhesive (4) is a backing plate (PVC plate) with adhesive.

[0039] The preparation method is as follows:

[0040] Preparation of pretreatment solution: prepare a 0.01 MPBS solution with a pH of 7.4 and set aside. Take part of the allocated PBS and add 20 mass volume % sucrose, add 2 mass volume % BSA and 0.02 mass volume % NaN 3 , after mixing thoroughly, set aside at 2-8°C.

[0041] Preparation of the mixture of markers: After coupling the amino group of fluorescent microspheres with the aldehyde group of dextran, add antibody 1 to mix at a mass ratio of 1:1, and o...

Embodiment 2

[0051] Example 2: Effect Verification

[0052] Standard curve fitting: Dilute the purchased progesterone standard with 0.01MpH7.4PBS to a linear concentration, and test the corresponding fluorescence intensity with progesterone detection test paper.

[0053] Table 1 control group and experimental group quantitative detection standard curve data table of the present invention

[0054]

[0055] Note: the control group is the comparative test paper of the embodiment; the experimental group of the present invention is the fluorescent immunochromatographic detection test paper obtained in the embodiment of the present invention.

[0056] Use the concentration and T / C to fit the trend line control group y=-0.13ln(x)+0.638, R 2 =0.994≥0.9801 correlation meets the standard; the experimental group of the present invention y=-0.15ln(x)+0.747, R 2 =0.997≥0.9801 The correlation meets the standard.

[0057] Sample: 4 different serums with progesterone concentrations of 50ng / ml, 25ng / ...

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Abstract

The invention discloses fluorescent immunochromatography test paper and a preparation method thereof. The test paper is formed by sequentially overlapping a sample pad (1), an antibody carrying film (2) and absorbent paper (3) through a base plate (4) which is provided with binding agents, the antibody carrying film (2) is coated with a marker mixture joint line (5), a testing line (6) and a quality control line (7) in sequence from the end close to the sample pad (1), and the marker mixture joint line (5) is formed by arranging a marker mixture obtained by coupling fluorescent microspheres with an antibody 1 and biotin within a pretreatment area (8) which is pretreated with the antibody carrying film in a coating mode through a crossed film metal-spraying instrument. By means of the test paper, the problems that the result is not accurate caused by insufficient release amount due to the fact that excessive binding materials remain on glass fibers are solved, the tested fluorescence value is raised, and the sensitivity or detection upper limit is lifted. Meanwhile, in the production, the antibody carrying film is coated with the marker mixture obtained by coupling the fluorescent microspheres with the antibody 1 and the biotin, the marker mixture joint line, the T line and the C line can be coated simultaneously, time and processes are saved, and the production efficiency is improved.

Description

technical field [0001] The invention relates to the field of detection test paper, in particular to a fluorescent immunochromatographic detection test paper and a preparation method thereof. Background technique [0002] Most of the clinically used test paper is composed of four parts, that is, the sample pad, the binding pad, the antibody-carrying membrane, and the absorbent paper are successively overlapped with each other through the bottom plate with adhesive, or the sample pad is directly made of glass fiber. membrane, omitting the conjugation pad. Wherein, the side of the binding pad close to the antibody-carrying membrane is coated or sprayed with the mixture of fluorescent microspheres, antibody 1 and biotin-conjugated label respectively. The T line and quality control C line of the antibody (or antigen) of the test item are coated on the antibody-carrying membrane. The fluorescent microspheres on the line react specifically with the mixture of antibody 1-conjugate...

Claims

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Application Information

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IPC IPC(8): G01N33/558
CPCG01N33/558
Inventor 颜珊杨莉莉吴颖张国锋
Owner XIAMEN BIOTIME BIOTECHNOLOGY CO LTD
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