Fluorescent immunochromatographic detection card, and preparation method and application thereof

A technology of fluorescence immunochromatography and detection card, which is applied in the field of fluorescence immunochromatography detection card and its preparation, can solve the problems of difficult detection, difficult NT-proBNP, low sensitivity, etc., achieve specificity and sensitivity improvement, and is not easy to quench off, high sensitivity effect

Inactive Publication Date: 2017-05-31
WUHAN NEWCANDO BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the extremely low concentration of NT-proBNP in serum, under normal circumstances, when using POCT method, the judgment standard is that NT-proBNP concentration > 300ng/L is positive, and the detection of heart failure marke...

Method used

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  • Fluorescent immunochromatographic detection card, and preparation method and application thereof
  • Fluorescent immunochromatographic detection card, and preparation method and application thereof

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Embodiment 1

[0032]A NT-proBNP fluorescent immunochromatography detection card of the present invention, comprising a treatment solution A, a treatment solution B and a detection card, wherein the treatment solution A contains antibody 15C4 coupled to fluorescent microspheres; the treatment solution B contains the antibody 15C4 coupled to The biotin antibody 13G12, the detection card includes a detection line area and a quality control line area, the detection line area is fixed with a streptavidin detection T line, and the quality control line area is fixed with a goat anti-mouse antibody quality control line C line.

[0033] The preparation method of the NT-proBNP fluorescent immunochromatography detection card of the above-mentioned present embodiment comprises the following steps:

[0034] (1) Preparation of treatment solution A:

[0035] (1.1) Take 200 μL of carboxy fluorescent microspheres (concentration: 10 mg / mL), and centrifuge (14400 rpm, 10 min). Take 1000 μL of pH6.0 MES buffe...

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Abstract

The invention discloses a fluorescent immunochromatographic detection card, and a preparation method and application thereof. The fluorescent immunochromatographic detection card comprises a treating fluid A, a treating fluid B and a detection card, wherein the treating fluid A contains an antibody 1 of a coupling fluorescent microsphere for an antigen to be detected; the treating fluid B contains an antibody 2 of a coupling biotin for the antigen to be detected; the detection card comprises a detection line area and a quality control line area; the detection line area is fixed with a streptavidin detection T line; the quality control line area is fixed with an antibody quality control C line. The preparation method comprises: (1) preparing the treating fluid A; (2) preparing the treating fluid B; (3) drawing lines on the detection card. The fluorescent immunochromatographic detection card has the characteristics of high sensitivity, high specificity, high stability and the like, and can be applied to quick detection of a disease mark.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a fluorescence immunochromatography detection card and its preparation method and application. Background technique [0002] At present, the methods for detecting disease markers mainly include radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), electrochemiluminescence immunoassay (ECLIA) and POCT. [0003] RIA due to extraction of samples or 125 Unstable I labeling often leads to large errors, relatively low sensitivity and specificity, and the determination method is complex, time-consuming, and requires a large sample size, which is not an ideal determination method. In recent years, enzyme-linked immunoassay has been widely used in the clinical diagnosis of diseases, but the reagent preparation of this method is difficult, the operation steps are complicated, time-consuming, there are many influencing factors, and the control is difficult to guarantee. The final ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/532G01N33/533
CPCG01N33/532G01N33/533G01N33/558G01N33/543G01N33/587G01N2333/58G01N2800/325G01N33/54388
Inventor 孙宏浩朱辉陈佳周昌荣王竞争
Owner WUHAN NEWCANDO BIOTECH CO LTD
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