Fluorescent immunochromatographic detection card, and preparation method and application thereof

Abstract

The invention discloses a fluorescent immunochromatographic detection card, and a preparation method and application thereof. The fluorescent immunochromatographic detection card comprises a treating fluid A, a treating fluid B and a detection card, wherein the treating fluid A contains an antibody 1 of a coupling fluorescent microsphere for an antigen to be detected; the treating fluid B contains an antibody 2 of a coupling biotin for the antigen to be detected; the detection card comprises a detection line area and a quality control line area; the detection line area is fixed with a streptavidin detection T line; the quality control line area is fixed with an antibody quality control C line. The preparation method comprises: (1) preparing the treating fluid A; (2) preparing the treating fluid B; (3) drawing lines on the detection card. The fluorescent immunochromatographic detection card has the characteristics of high sensitivity, high specificity, high stability and the like, and can be applied to quick detection of a disease mark.

Description

technical field [0001] The invention relates to the technical field of immunoassay, in particular to a fluorescence immunochromatography detection card and its preparation method and application. Background technique [0002] At present, the methods for detecting disease markers mainly include radioimmunoassay (RIA), enzyme-linked immunoassay (ELISA), electrochemiluminescence immunoassay (ECLIA) and POCT. [0003] RIA due to extraction of samples or 125 Unstable I labeling often leads to large errors, relatively low sensitivity and specificity, and the determination method is complex, time-consuming, and requires a large sample size, which is not an ideal determination method. In recent years, enzyme-linked immunoassay has been widely used in the clinical diagnosis of diseases, but the reagent preparation of this method is difficult, the operation steps are complicated, time-consuming, there are many influencing factors, and the control is difficult to guarantee. The final ...

AI Technical Summary

Problems solved by technology

Due to the extremely low concentration of NT-proBNP in serum, under normal circumstances, when using POCT method, the judgment standard is that NT-proBNP concentration > 300ng / L is positive, and the detection of heart failure markers prepared by traditional immunofluorescence technology is stuck in the When detecting NT-proBNP, the sensitivity is low, and the extremely low concentration of NT-proBNP is difficult to be captured by the T line and difficult to detect

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