Biosensor, preparation method and target molecule concentration detection method thereof

A technology of biosensors and target molecules, which is applied in biological testing, instruments, measuring devices, etc., can solve the problems of limiting the application of antibody antigens, time-consuming, and reduced activity, and achieves a simple and fast detection process, no immunogenicity, and improved The effect of stability

Inactive Publication Date: 2017-12-12
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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Problems solved by technology

But there are also some disadvantages, such as time-consuming, high cost, immunogenicity and chemical modification of the produced antibodies may lead to reduced activity or even inactivation, which limits the application of antibody antigens in protein analysis

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  • Biosensor, preparation method and target molecule concentration detection method thereof
  • Biosensor, preparation method and target molecule concentration detection method thereof
  • Biosensor, preparation method and target molecule concentration detection method thereof

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Embodiment Construction

[0029] The concept of the present invention is: the nucleic acid aptamer (Apatamer) is a single-stranded nucleic acid molecule with a specific recognition function, which can be synthesized in vitro through the ligand system evolution technology of exponential enrichment. Through research, it is found that in the presence of a target (that is, a target molecule), aptamers will undergo adaptive folding to form three-dimensional structures, such as G-quadruplexes, hairpins, convex rings, and pseudoknots. Tightly bind to the target through complementary base pairing, shape complementarity, van der Waals force, electrostatic interaction and hydrogen bond. There is a one-to-one correspondence between the target and its nucleic acid aptamer. For example, target A will fold its corresponding aptamer B to form a three-dimensional structure; target A1 will fold the corresponding aptamer B1 to form a three-dimensional structure. Furthermore, in contrast to antibodies, aptamers are easy ...

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Abstract

The invention discloses a biosensor, a preparation method and a target molecule concentration detection method thereof. The preparation method for the biosensor comprises the following steps: (S1) modifying one or more of streptavidin, carboxyl, amino and sulfydryl onto a magnetic bead and modifying biotin onto a nucleic acid aptamer of a to-be-detected target molecule; (S2) adding the nucleic acid aptamer and the magnetic bead into a reaction solution and coupling the nucleic acid aptamer onto a local site of the magnetic bead, wherein the reaction solution is a salt buffer solution in concentration above 100mM; (S3) coupling the DNA sequence capable of complementarily pairing with the nucleic acid aptamer onto a noble metal nanoparticle; and (S4) reacting the magnetic bead acquired in the step S2 and the noble metal nanoparticle acquired in the step S3 for 5-60min in a hybridization solution, thereby forming the biosensor by the magnetic bead, nucleic acid aptamer and noble metal nanoparticle under the effect of base complementary pairing. The biosensor is capable of realizing high-sensitivity concentration detection, is low in cost and is high in efficiency.

Description

【Technical field】 [0001] The invention relates to biological detection technology, in particular to a biosensor, a preparation method thereof, and a method for detecting the concentration of target molecules. 【Background technique】 [0002] A biomarker is an indicator of the biological state of a disease, which can be a fragment of protein, DNA or RNA, and has specificity, so it can be used as a marker for targeting a disease. Cancer biomarkers, in particular, are signs of cancer. By detecting them, cancer can be diagnosed. With the development of biotechnology, many cancer biomarkers have been discovered. Through the detection of these biomarkers, diseases can be diagnosed and treated at the expected time. Significant changes in the protein can reveal the physiological and pathological process of the organism as a biomarker, indicating the biological state of the disease. These proteins are of great significance in basic research of life sciences, disease diagnosis, dru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/68
CPCG01N33/531G01N33/68
Inventor 孙树清杨瑞吴振杰
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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