Content analysis and detection method of twelve compound vitamins for injection
A compound vitamin and detection method technology, which is applied in the field of content analysis and detection of 12 compound vitamins for injection, can solve the problems of large detection interference, complex physical and chemical properties, poor specificity and sensitivity of vitamins, etc., and achieve improved detection efficiency and high Effect of Detection Sensitivity
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Embodiment 1
[0049] Vitamin A Palmitate, Vitamin D 3 Determination with racemic α-tocopherol according to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition two appendix VD). (Operation in dark)
[0050] Chromatographic conditions and system suitability test use octadecylsilane bonded silica gel as filler (4.6×250mm), column temperature is 40°C, methanol-ethanol (15:25) is mobile phase A, acetonitrile-water (60 : 5) is mobile phase B, carry out gradient elution according to the following procedures:
[0051]
[0052] The total flow rate is 1.0ml / min, the detection wavelength is 265nm, and the number of theoretical plates is calculated according to the vitamin A palmitate peak, which should not be less than 4000, vitamin D 3 The resolution between the peak and the peak of racemic α-tocopherol and between the peak of vitamin A palmitate and the peak of racemic α-tocopherol should meet the requirements.
[0053] Preparation of reference substance solution Take vi...
Embodiment 2
[0058] Cocarboxylase tetrahydrate, riboflavin sodium phosphate, vitamin B 6 , vitamin C, nicotinamide, folic acid, and dexpanthenol were determined according to high-performance liquid chromatography (Appendix VD of the second part of the Chinese Pharmacopoeia 2010 edition). (Operation in dark)
[0059] Chromatographic conditions and system suitability test use Inertsil 5μODS24.6×250mm chromatographic column, column temperature is 40°C, 0.1% phosphoric acid solution of 0.005mol / L sodium pentanesulfonate is used as mobile phase A, 0.005mol / L pentanesulfonate 0.1% phosphoric acid solution of sodium bicarbonate-acetonitrile (20:80) was used as mobile phase B, and the gradient elution was carried out according to the following procedure:
[0060]
[0061] The total flow rate is 1.0ml / min, the detection wavelength is 210nm, the number of theoretical plates calculated based on the riboflavin sodium phosphate peak should not be less than 15,000, and the separation between each pe...
Embodiment 3
[0067] Biotin was determined according to high performance liquid chromatography (Chinese Pharmacopoeia 2010 edition two appendix VD).
[0068] Inertsil 5μODS24.6×250mm chromatographic column for chromatographic conditions and system suitability test, column temperature 40°C, dipotassium hydrogen phosphate buffer solution (accurately weigh 1.132g of dipotassium hydrogen phosphate, add water to dissolve and dilute to 3000ml, use phosphoric acid solution (1→10) adjust the pH to 3.00±0.02) as mobile phase A, methanol as mobile phase B, and carry out gradient elution according to the following procedure.
[0069]
[0070] The total flow rate is 1.2ml / min, the detection wavelength is 210nm, and the number of theoretical plates should not be less than 1800 based on the biotin peak.
[0071]Preparation of reference substance solution Take about 17.25 mg of biotin reference substance, weigh it accurately, put it in a 50ml measuring bottle, add an appropriate amount of water and a l...
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