A fluorescence immunochromatographic detecting method for anti-mullerian hormone and a kit

Abstract

The invention relates to a fluorescence immunochromatographic detecting method for anti-mullerian hormone and a kit thereof. The method includes steps of preparing a probe fixing pad, preparing an immunochromatographic test paper strip, preparing a sample diluting liquid and detecting a sample, wherein the probe fixing pad is prepared by mixing anti-mullerian hormone monoclonal antibody fluorescence latex microparticles and goat anti-rabbit IgG fluorescence latex microparticles, diluting with a gold diluting liquid and spraying glass fibers with the diluted solution. A detection line is covered with an anti-mullerian hormone monoclonal antibody, and a control line is covered with goat anti-rabbit IgG so as to prepare the immunochromatographic test paper strip. The kit comprises an immunochromatographic detecting card. The immunochromatographic detecting card comprises a PVC liner plate, a sample pad, the probe fixing pad, a nitrocellulose membrane and water absorbing paper. The probe fixing pad is prepared by mixing the anti-mullerian hormone monoclonal antibody fluorescence latex microparticles and the goat anti-rabbit IgG fluorescence latex microparticles and drying. The method and the kit are advantaged by high detection sensitivity, high accuracy, simple operation and a low cost.

Description

technical field [0001] The invention relates to a detection method and kit for anti-Müllerian hormone, in particular to a fluorescent immunochromatographic detection method for anti-Müllerian hormone and a detection kit. Background technique [0002] The traditional evaluation of ovarian function mainly uses age, follicle stimulating hormone (FSH), inhibin B (InhibinB), estrogen (E2), number of antral follicles (Antralfollicle count), ovarian size (Ovarian volume) etc. as indicators, relying on a single indicator Can not truly reflect the ovarian condition. In recent years, studies have found that Anti-Mullerian hormone (AMH) is also involved in the regulation of female ovarian function. It plays a certain role in the growth of follicles and can be used as a marker for the type and degree of abnormal ovarian function in anovulatory women. AMH released by ovarian granulosa cells can be detected in serum, and the level of AMH is proportional to the number of developing follic...

AI Technical Summary

Problems solved by technology

However, the detection steps of this method include coating and sealing of ELISA plates, processing of standard products or serum to be tested, and detection of monoclonal antibodies. During the detection process, the incubation time is long and the number of washing plates is high, which requires supporting equipment and professionals.

[0006] Chinese patent CN104730247A discloses a kit suitable for the rapid detection of AMH and INHB by double-labeled time-resolved fluorescence immunoassay, using lanthanide-labeled AMH detection antibodies, and using AMH immobilized on a solid-phase carrier to coat antibodies and antigens AMH effect, the fluorescent substance is fixed on the solid phase carrier to achieve the purpose of detecting AMH. However, this method requires incubation and washing of the plate in operation, and also has the disadvantages of complicated operation and low detection sensitivity.

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