Fluorescence immunochromatographic assay method of serum amyloid protein A and kit

A fluorescence immunochromatography, serum starch technology, applied in biological testing, measuring devices, analytical materials, etc., can solve the problem that the accurate and quantitative SAA content cannot meet the demand, etc., and achieve the effects of low cost, reduced space resistance, and simple detection equipment.

Inactive Publication Date: 2016-06-15
SUZHOU BIONANOTECH CO LTD
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This method is fast and simple, and the test results are easy to judge. It is suitable for quali

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  • Fluorescence immunochromatographic assay method of serum amyloid protein A and kit
  • Fluorescence immunochromatographic assay method of serum amyloid protein A and kit

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[0041] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Apparently, the described embodiments are only a part of the embodiments of the present invention, not all of them, and are not intended to limit the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

[0042] The fluorescent immunochromatographic detection method of serum amyloid A of the present invention comprises the following steps:

[0043] Step 1) Probe immobilization pad preparation: add the activator 1-(3-dimethylaminopropyl)-3-ethylcarbodiazyme to the solution of fluorescent latex particles with carboxyl or amino groups with an emission wavelength of 500-590nm Amine hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were reacted at room te...

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Abstract

The invention relates to a fluorescence immunochromatographic method of serum amyloid protein A and a detection kit thereof. The method includes the steps of preparing a probe fixing pad, preparing an immunochromatographic test strip, preparing a sample diluting liquid and detecting a sample. The probe fixing pad is prepared by mixing serum amyloid protein A monoclonal antibody fluorescent latex particles and goat-anti-rabbit IgG fluorescent latex particles, and diluting the mixture with a gold diluent and spraying the liquid onto glass fibers. A detection line is coated with serum amyloid protein A monoclonal antibody and a control line is coated with goat-anti-rabbit IgG to prepare the immune-chromatographic test strip. The detection kit includes an immune-chromatographic detection card including a PVC lining plate, a sample pad, the probe fixing pad, a nitrocellulose membrane and a water absorbing paper. The probe fixing pad is prepared by mixing and drying the serum amyloid protein A monoclonal antibody fluorescent latex particles and the goat-anti-rabbit IgG fluorescent latex particles, so that the detection kit is high in detection sensitivity and accuracy, is simple in operation and is low in cost.

Description

technical field [0001] The invention relates to a detection method and kit for serum amyloid A, in particular to a fluorescent immunochromatographic detection method for serum amyloid A and a detection kit thereof. Background technique [0002] Serum amyloid A (serumamyloid A protein, SAA) is a kind of polymorphic protein family encoded by multiple genes. In the acute phase response, such as the acute phase of inflammation or infection, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, and the concentration within 48 to 72 hours It increases rapidly, and can increase to 100-1000 times the initial concentration, but its half-life is short, only about 50 minutes, and it decreases rapidly during the recovery period of the disease. For certain diseases, such as viral infection, transplant rejection, coronary heart disease, etc., the sensitivity of SAA is higher than that of C-reactive protein (CRP), which can provide bet...

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893
Inventor 张华廖平璋张鹏张金林
Owner SUZHOU BIONANOTECH CO LTD
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