Fluorescence immunochromatographic assay method of serum amyloid protein A and kit

Abstract

The invention relates to a fluorescence immunochromatographic method of serum amyloid protein A and a detection kit thereof. The method includes the steps of preparing a probe fixing pad, preparing an immunochromatographic test strip, preparing a sample diluting liquid and detecting a sample. The probe fixing pad is prepared by mixing serum amyloid protein A monoclonal antibody fluorescent latex particles and goat-anti-rabbit IgG fluorescent latex particles, and diluting the mixture with a gold diluent and spraying the liquid onto glass fibers. A detection line is coated with serum amyloid protein A monoclonal antibody and a control line is coated with goat-anti-rabbit IgG to prepare the immune-chromatographic test strip. The detection kit includes an immune-chromatographic detection card including a PVC lining plate, a sample pad, the probe fixing pad, a nitrocellulose membrane and a water absorbing paper. The probe fixing pad is prepared by mixing and drying the serum amyloid protein A monoclonal antibody fluorescent latex particles and the goat-anti-rabbit IgG fluorescent latex particles, so that the detection kit is high in detection sensitivity and accuracy, is simple in operation and is low in cost.

Description

technical field [0001] The invention relates to a detection method and kit for serum amyloid A, in particular to a fluorescent immunochromatographic detection method for serum amyloid A and a detection kit thereof. Background technique [0002] Serum amyloid A (serumamyloid A protein, SAA) is a kind of polymorphic protein family encoded by multiple genes. In the acute phase response, such as the acute phase of inflammation or infection, stimulated by IL-1, IL-6 and TNF, SAA is synthesized in the liver by activated macrophages and fibroblasts, and the concentration within 48 to 72 hours It increases rapidly, and can increase to 100-1000 times the initial concentration, but its half-life is short, only about 50 minutes, and it decreases rapidly during the recovery period of the disease. For certain diseases, such as viral infection, transplant rejection, coronary heart disease, etc., the sensitivity of SAA is higher than that of C-reactive protein (CRP), which can provide bet...

AI Technical Summary

Problems solved by technology

This method is fast and simple, and the test results are easy to judge. It is suitable for qualitative detection, but it still cannot meet the demand for accurate quantification of SAA content.

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