Method for quantitative detection of immunochromatographic test card

A technology of immunochromatographic test paper and quantitative detection method, which is applied in the field of analytical chemistry, can solve problems such as baseline offset, false signal peak interference signal-to-noise ratio, etc., to improve accuracy, enhance signal-to-noise ratio, improve accuracy and sensitivity Effect

Active Publication Date: 2014-10-15
广纳达康(广州)生物科技有限公司
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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides a quantitative detection method for immunochromatography test paper cards, the purpose of which is to eliminate interference through dual lig...

Method used

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  • Method for quantitative detection of immunochromatographic test card
  • Method for quantitative detection of immunochromatographic test card
  • Method for quantitative detection of immunochromatographic test card

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Embodiment 1

[0051] This embodiment relates to a quantitative detection method of a colloidal gold immunochromatography test paper card, and the immunochromatography paper card used uses colloidal gold particles as the carrier of the immune marker. Specifically, in this embodiment, human chorionic gonadotropin (HCG) colloidal gold immunochromatography test paper card is used. Drop 150 μL of the sample to be tested into the sample hole of the HCG immunochromatography test paper card, and let it stand for 10 minutes for chromatography to obtain the immunochromatography test paper card to be tested. The T line of the test line and the C line of the quality control line will appear on the test paper card in the window. The higher the HCG content in the sample, the darker the color of the T line.

[0052] The detection optical path is constructed as follows:

[0053] Since the colloidal gold particles used in this embodiment have a unique maximum absorption peak (peak wavelength 525nm) in the...

Embodiment 2

[0077] This embodiment relates to a quantitative detection method of latex particle immunochromatography test paper card, the immunochromatography paper card used uses blue latex particles as the carrier of the immune marker. Specifically, in this embodiment, a chloramphenicol (CAP) immunocompetitive chromatography test paper card is used. Add 150 μL of the sample to be tested dropwise into the sample hole of the CAP immunochromatography test paper card, and let it stand for 15 minutes for chromatography to obtain the immunochromatography test paper card to be tested. in the window. The higher the chloramphenicol content in the sample, the lighter the color of the test line T line.

[0078] The detection optical path is constructed as follows:

[0079] Since the blue latex particles used in this embodiment have a unique maximum absorption peak (peak wavelength 607nm) in the wavelength range of 585-630nm, and have no obvious absorption at wavelengths below 540nm, the characte...

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Abstract

The invention discloses a method for quantitative detection of an immunochromatographic test card. The method comprises the following steps: (1) placing an immunochromatographic test card to be tested on a detection light path of a first light source to acquire a first image data matrix Pic 1; (2) placing the immunochromatographic test card to be tested on a detection light path of a second light source to acquire a second image data matrix Pic 2; (3) selecting a reference zone to calculate the image difference matrix a Pic; (4) adopting the chromatographic peak detection algorithm to calculate the characteristic value; (5) calibrating the concentration of an object to be tested. The method for the quantitative detection of the immunochromatographic test card can eliminate the problem of baseline deflection caused by nonuniform lighting of the light paths, dead angles of a lens of a detector and the like, and meanwhile, can effectively inhibit spurious signal peaks brought by shadows, impurity particle interferents and the like, thereby improving the accuracy in distinguishing chromatographic peaks, enhancing the signal-to-noise ratio of the chromatographic peak of an immune marker and improving the accuracy and the sensitivity.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a quantitative detection method of an immunochromatography test paper card. Background technique [0002] Immunochromatography test card rapid detection technology is a simple and rapid on-site rapid detection method, which can complete an immune test within 5 to 15 minutes, and is widely used in medical diagnosis, food safety detection, environmental monitoring and other fields. According to the different types of immune markers, there are currently a variety of products such as colloidal gold test paper cards, latex particle chromatography test cards, gold-magnetic composite particle chromatography test paper cards, among which colloidal gold test paper cards are the most widely used. [0003] In order to realize the quantitative detection of the immunochromatography test paper card, it is necessary to use specific optical detection equipment, illuminate the ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N21/31
CPCG01N21/3151G01N33/558
Inventor 杨海石雄飞张怀雯宋谦金伟
Owner 广纳达康(广州)生物科技有限公司
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