Lung cancer marker detection immunochromatographic test paper and application

Abstract

The invention belongs to the cancer detection technology, relating to a lung cancer marker detection immunochromatographic test paper and an application. The detection of a single tumour marker in the prior art can not meet the requirements of clinical early diagnosis and differential diagnosis. The invention realizes the early diagnosis of lung cancer by jointly detecting NSE and CEA in serum with a double antibody sandwich immunochromatography. The invention comprises the following preparation steps: preparing aurosol-neure NSE and aurosol-carcinoembryonic antigen CEA antibody compounds; preparing double antibody sandwich NSE and CEA detection test strips. The invention comprises the following steps in lung cancer detection: configurating series concentration NSE and CEA standard substances; preparing NSE and CEA mixed antigen solution with serum of normal people; inserting the sample pad ends of the test strips into the above concentration series standard substances respectively to observe the changes in colours of T and C lines on a NC film. The invention has the advantages of convenient detection operation, high sensitivity and precise result, and can determine lung cancer recurrence 4-12 weeks ahead.

Description

technical field

[0001] The invention belongs to cancer detection technology, in particular to an immunochromatographic test paper for detection of lung cancer markers and its application. Background technique

[0002] Lung cancer is the most common malignant tumor with the highest morbidity and mortality rate and poor treatment effect. Because there are no specific clinical symptoms and manifestations in the early stage of the disease, most of the patients are already in the late stage when they have clinical manifestations, and the clinical treatment effect and median survival time are not satisfactory. Therefore, early detection and diagnosis of lung cancer is of great significance to the treatment and prognosis of lung cancer.

[0003] Neuron-specific enolase (NSE) is an acidic protease unique to neurons and neuroendocrine cells. The content in lung cancer tissue is 3-35 times that in normal lung tissue; The most sensitive and specific tumor marker; it is also a tumor m...