Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Performance correction method for gold mark immunochromatographic test strip detection system

An immunochromatography test strip and detection system technology, which is applied in the field of performance calibration of the gold standard immunochromatography test strip detection system, can solve the problems of the detection system such as large measurement errors, inconvenient operation, and data that cannot be processed in real time, so as to reduce the measurement error, to ensure the effect of accurate quantitative measurement

Active Publication Date: 2012-10-03
SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
View PDF6 Cites 28 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of the above-mentioned prior art 1 are: ① The measurement error introduced by the aging of the system (such as the aging of the LED of the light-emitting part, the photodiode of the receiving part, and electronic components, etc.) is not considered
② Due to the use of multiple independent photodiode pixels, the conditions for data acquisition are not consistent, and measurement errors may be introduced
Especially when the photodiode pixels in the photodiode array are aging, the detection system will produce greater measurement errors due to the different aging conditions of each photodiode pixel
③Although the aperture part of the system is set to block the scattered light in other areas, it is impossible to completely eliminate the influence of these scattered light
The main disadvantages of the above-mentioned prior art 2 are: ① before collecting the test data, the measurement error introduced by the components of the system due to aging and other problems was not considered
②The use of a large number of additional circuits will definitely affect the stability of the instrument
The main disadvantages of the above-mentioned prior art 3 are: ① The measurement errors introduced by the components of the system due to problems such as aging are ignored
Furthermore, when collecting test data, it is difficult for the results to reflect the real situation
② Without any corrective measures, the consistency of test conditions cannot be guaranteed
③The data cannot be processed in real time and requires the assistance of the host computer, which brings inconvenience to the operation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Performance correction method for gold mark immunochromatographic test strip detection system
  • Performance correction method for gold mark immunochromatographic test strip detection system
  • Performance correction method for gold mark immunochromatographic test strip detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A photodiode is adopted as a kind of photodetector, and the present invention is described in detail by taking a photodiode as an example.

[0039] see figure 1 , figure 1 It is a structural schematic diagram of the gold standard test strip detection system involved in the present invention. Depend on figure 1 It can be seen that the gold standard test strip detection system includes a gold standard strip color card 1, a photodiode 2, a signal processing system 3, lighting components 4, 5, an imaging optical system 6 and a moving component 7. The signal processing system 3 is mainly used for collecting and calculating the output signal of the photodiode 2 and controlling the photodiode 2 . The collected signal is calculated by the algorithm to obtain the standard data. The moving part 7 is mainly used for the translation of the gold label color card 1 .

[0040] The shape and structure of the gold standard strip color card 1 is exactly the same as the gold standard...

Embodiment 2

[0057] The image sensor is a kind of photodetector, and the present invention will be described in detail by taking the image sensor as an example.

[0058] see figure 2 , figure 2 It is a structural schematic diagram of the gold standard test strip detection system involved in the present invention. Depend on figure 2 It can be seen that the detection system is composed of a gold label color card 1 , an image sensor 2 , a signal processing system 3 , lighting components 4 and 5 and an imaging optical system 6 .

[0059] The gold label color chart 1 is the same as that described in Example 1.

[0060] see Figure 4 , Figure 4 It is a structural schematic diagram of the colloidal gold immunochromatography test strip involved in the present invention. Depend on Figure 4 It can be seen that 83, 84 and 85 are non-functional areas. 81, 82 represent the functional area of ​​the test strip.

[0061] The signal processing system 3 is mainly used to collect the output dat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a performance correction method for a gold mark immunochromatographic test strip detection system. The method comprises the steps of: first, recording data information corresponding to a gold mark strip colorimetric card by a photoelectric detector of the gold mark immunochromatographic test strip detection system; then determining and storing the optimal response curve of the data of the gold mark strip colorimetric card and the concentration of the object detected according to the corresponding relation between the color of the gold mark strip colorimetric card and the concentration of the detected substance; then, using the gold mark strip colorimetric card to test current state of the gold mark test strip system when performance of the gold mark test strip system is needed to be corrected, determining a current response curve of the gold mark test strip system, then comparing the optimal response curve with linear sections of the current response curve one by one and section by section; determining and storing the mapping functions corresponding to the linear sections of the current response curve; and finishing correction. According to the performance correction method for the gold mark immunochromatographic test strip detection system provided by the invention, the truth-value of the concentration of the object detected can be obtained by calculating the data collected by the gold mark test strip system, thereby reducing the measurement error caused by problems of system aging and the like, and ensuring long time accurate and quantitative measurement of the gold mark test strip system.

Description

technical field [0001] The invention belongs to the technical field of immunodetection, in particular to a performance correction method of a gold standard immunochromatographic test strip detection system. Background technique [0002] Gold label immunochromatography technology is a rapid on-site detection technology that combines colloidal gold labeling technology and immunochromatography technology. In recent years, it has been widely used in various biological fields, such as medical diagnosis, environmental and food safety monitoring and scientific research. Colloidal gold is used as a label in almost all immunoblotting techniques used clinically. [0003] Colloidal gold immunochromatographic test strips (hereinafter referred to as gold-labeled test strips) are the carrier of the immunochromatographic reaction. The gold-labeled test strips are filled with nano-gold particles that combine with the target object. Gold particles have light-absorbing properties, and when ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/558
Inventor 黄立华陈贝特郭凯黄惠杰
Owner SHANGHAI INST OF OPTICS & FINE MECHANICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com