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Multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1 and preparation method thereof

An immunochromatographic test paper and aflatoxin technology, which can be used in measuring devices, analytical materials, biological material analysis, etc., and can solve the problem of low sensitivity

Inactive Publication Date: 2010-12-01
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional aflatoxin B1 immunochromatographic test strip has only one detection line, which can only qualitatively detect aflatoxin B1 in the sample, and the sensitivity is low

Method used

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  • Multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1 and preparation method thereof
  • Multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1 and preparation method thereof
  • Multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1 and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-3

[0047] Example 1-3: Preparation and application of highly sensitive immunochromatographic test paper for semi-quantitative detection of aflatoxins

[0048] The commercially available aflatoxin B1 monoclonal antibody A9555 was used in the following Examples 1-3, but is not limited to the antibody used in the following examples. Other aflatoxin B1 antibodies are also suitable, but their detection sensitivity may be different.

Embodiment 1

[0050] A method for preparing a highly sensitive immunochromatographic test strip for semi-quantitative detection of aflatoxin B1, comprising the following steps:

[0051] (1) Preparation of absorbent pad

[0052] Cut the absorbent paper to a size of 16mm and 3mm wide to obtain an absorbent pad;

[0053] (2) Preparation of detection pad

[0054] The coating of the detection line:

[0055] Prepare the commercially available aflatoxin B1-bovine serum albumin conjugate AFB1-BSA into 0.1mg / mL coating solution A, and spray it at positions 13mm, 15mm, and 17mm from the edge of the nitrocellulose membrane. The coating solution A is coated horizontally on the nitrocellulose membrane sequentially to obtain the detection line I, the detection line II and the detection line III, and the aflatoxin B1-bovine serum on the detection line I, the detection line II and the detection line III per centimeter The coated amounts of albumin conjugate AFB1-BSA were 120ng, 40ng and 20ng respectivel...

Embodiment 2

[0079] A method for preparing a highly sensitive immunochromatographic test strip for semi-quantitative detection of aflatoxin B1, comprising the following steps:

[0080] (1) Preparation of absorbent pad

[0081] Cut the absorbent paper to a size of 17mm and 2mm wide to obtain an absorbent pad;

[0082] (2) Preparation of detection pad

[0083] The coating of the detection line:

[0084] Prepare the commercially available aflatoxin B1-bovine serum albumin conjugate AFB1-BSA into 0.25mg / mL coating solution A, and spray it at positions 15mm, 17mm, and 19mm from the edge of the nitrocellulose membrane. The coating solution A is coated horizontally on the nitrocellulose membrane successively to obtain the detection line I, detection line II and detection line III, and the required aflatoxin B1- Coating amounts of bovine serum albumin conjugates were 300ng, 100ng and 50ng respectively, and then dried at 38°C for 10 minutes;

[0085] The coating solution A is 25mg commercially ...

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Abstract

The invention belongs to the field of bioinstrumentation and relates to a multi-test-line immunochromatographic test strip for semi-quantitatively detecting aflatoxin B1, comprising a paperboard, wherein a water absorbent pad, a detection pad, a gold-labeled pad and a sample pad are adhered on one surface of the paperboard from top to bottom in sequence, wherein adjacent pads are overlapped and connected at the connection part, the detection pad takes a nitrocellulose film as a base pad, the nitrocellulose film is provided with a transverse quality control line, a detection line I, a detection line II and a detection line III from top to bottom, wherein the quality control line is wrapped with a rabbit anti-mouse polyclonal antibody, and the detection line I, the detection line II and the detection line III are respectively wrapped with an aflatoxin B1-bovine serum albumin (AFB1-BSA) conjugate; and the gold-labeled pad is transversely sprayed with a nanogold-labeled aflatoxin B1 monoclonal antibody. The immunochromatographic test strip is used for semi-quantitatively detecting the aflatoxin B1 and has the characteristics of fast detection, simple operation and high sensitivity.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to a multi-detection-line immunochromatographic test strip for semi-quantitative detection of aflatoxin B1 and a preparation method thereof. Background technique [0002] Aflatoxin is mainly a secondary metabolite secreted by Aspergillus flavus and Aspergillus parasiticus, and is a natural toxic compound that can cause various damages to humans and animals. Among the aflatoxins that have been discovered, aflatoxin B1 (AFB1 for short) is the most toxic aflatoxin, and its toxicity, carcinogenicity and pollution frequency all rank first in biological toxins. [0003] After aflatoxin contaminates food and feed, it will directly or indirectly enter the human food chain, threatening human health and life safety, and the degree of harm is proportional to the intake of aflatoxin. Aflatoxin B1 widely exists in agricultural products such as rice, corn, peanuts, sesame, soybean...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/532
CPCG01N33/56961G01N2333/38
Inventor 李培武张道宏张奇张文丁小霞姜俊陈小媚
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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