31 results about "Cardiac marker" patented technology

An implantable biosensor system is disclosed for determining levels of cardiac markers in a patient to aid in the diagnosis, determination of the severity and management of cardiovascular diseases. The sensor includes nanowire sensor elements having a biological recognition element attached to a nanowire transducer that specifically binds to the cardiac marker being measured. Each of the sensor elements is associated with a protective member that prevents the sensor element from interacting with the surrounding environment. At a selected time, the protective member may be disabled, thereby allowing the sensor element to begin sensing signals within a living body.

The present invention provides for the detection of cardiac markers on a droplet actuator. An aspect provides a method of assaying a cardiac marker in a biological sample from a subject, the method including providing a droplet actuator, loading the biological sample and assay reagents on the droplet actuator, executing droplet operations to create sample droplets from the sample and reagent droplets from the reagents on the droplet actuator, and executing droplet operations using the sample droplets and reagent droplets to produce a detectable signal indicative of the quantity of the cardiac marker in the biological sample. Still other aspects are provided.

Methods for detecting cardiac injury by detecting one or more cardiac markers are provided. A plurality of particles, each of which is coated with a capture agent having an affinity for a cardiac marker, is combined with the sample to form a plurality of analyte-particle complexes. The system also includes a transport arrangement for transporting the sample to the sensor surface, and optionally a magnetic field inducing structure constructed and arranged to establish a magnetic field at and adjacent to the sensor surface. The resonant sensor produces a signal corresponding to an amount of analyte-particle complexes that are bound to the sensor surface.

Methods and apparatus are provided for documenting the myocardial ischemia of a patient's heart. The apparatus comprises an ECG monitor and data collector configured to receive electrocardial data about the patient's heart. The apparatus further comprises a cardiac marker data collector configured to receive cardiac marker data about the patient's heart. A data processing and recording module is in electrical communication with the ECG monitor and data collector and the cardiac marker data collector and is configured to record the electrocardial data and the cardiac marker data. The method comprises the steps of obtaining electrocardial data about the patient's heart and receiving results of a cardiac marker test performed on the patient. The electrocardial data and the test results are stored in a patient report, which then may be displayed.

The embodiment of the invention discloses a micro-fluidic chip based on a magnetic bead coated antibody and a method for capturing cardiac markers. The micro-fluidic chip based on the magnetic bead coated antibody and the method for capturing cardiac markers are applied to the field of immunomagnetic bead coated binding antibodies, and prevent coated antibodies from being redissolved in a fluid system or being captured by antigens in fluid and disengaged from a base material. The micro-fluidic chip comprises a sample injection chamber, a Y-shaped structure channel and recycling chambers. The Y-shaped structure channel comprises a funnel-shaped channel at the upper portion and a columnar channel at the lower portion. An immunolabelling module is arranged at the bottom of the funnel-shaped channel. A blood filtering module is arranged at the upper portion of the immunolabelling module. The bottom end of the columnar channel at the lower portion is forked to form a pair of parallel immunity channels. The tail end of each immunity channel is connected with one recycling chamber. The columnar channel is provided with an upper cavity and a lower cavity. The upper cavity is a strong magnetism cavity, and the lower cavity is a quality control cavity.

The invention discloses a method, system and chip test paper for parallel detection on various cardiac markers. The chip test paper is used for detecting a part or all of the cardiac markers including cTNI (cardiac troponin I), cTNT (cardiac troponin T), MYO (myoglobin), CK-MB (isoenzymeof creatine kinase containing M and B subunits), BNP (B-type natriuretic peptide), CRP (C-reactive protein) and FABP (fatty acid-binding protein); the chip test paper comprises a first membrane and a second membrane; a ligand An of each marker is arranged on the first membrane, and a ligand Bn coupled with a signal marker is absorbed on the second membrane; and a detected material forming sandwich detection together with the ligands An and Bn is added from a sampling hole, then under the acting force of percolation or other factors, the detected material moves to be combined with the ligands An and Bn respectively to form a composite array of the first membrane-the ligand An-the detected material-the ligand Bn-the signal marker, which is fixed on the first membrane, the composite array and a capture fiber membrane are assembled simultaneously, and a detection hole is reserved. The method, the system and the chip test paper provided by the invention can be used for detecting the various cardiac markers simultaneously and quantitatively; moreover, the detection sensitivity can be improved, and the detection time is saved.

The invention relates to the field of in-vitro diagnosing medical appliances, particularly relates to a detection device, as well as a preparation method and application thereof, and further disclosesa POCT diagnostic card. The diagnostic card integrates a calibration reagent, a reagent valve, an electrode array chip, a waste liquid storage cavity and the like, so that reagent sampling is not required in the detecting process, no extra medical waste is generated, and all fluids and pipelines are integrated on the test card, so that use convenience is greatly improved, and the risk of fluid path blockage of an instrument can be avoided. The diagnostic card applies the electrochemical principle, can be used for performing in-vitro diagnosis on patients and analyzing certain biological and chemical substances in human blood samples, and can be applied to multiple medical diagnosing fields, such as biochemical item tests, blood-qi item tests, metabolin tests, hematological tests, clottingtests and immune tests (cardiac markers and the like).

The invention relates to a fluorescent microsphere combined detection device for a cardiac marker and a preparation method of the fluorescent microsphere combined detection device, and belongs to thefield of medical detection equipment. A sample pad, an immunofluorescent antibody glass fiber membrane, a nitrocellulose membrane and an absorption pad respectively adhere to a plastic plate, the twoends of the nitrocellulose membrane are respectively overlapped with the absorption pad and the immunofluorescent antibody glass fiber membrane, the other end of the immunofluorescent antibody glass fiber membrane is overlapped with the sample pad, the nitrocellulose membrane is provided with a first detection line T1 with a solid-phase high-specificity MPO antibody, a second detection line T2 with a solid-phase high-specificity cTnI antibody, a third detection line T3 with a solid-phase high-specificity NT-proBNP antibody, a fourth detection line T4 with a solid-phase high-specificity D dimerantibody and a quality control line C; and a goat anti-mouse IgG polyclonal antibody is sprayed on the quality control line C. The fluorescent microsphere combined detection device for the cardiac marker has the advantages that the sensitivity and the specificity of reaction are effectively improved, the use amount of immune microspheres can further be reduced under the same threshold value, thecost is saved, and the device is simple and convenient to operate and high in practicability.

The invention belongs to the field of in-vitro diagnosis and particularly relates to a quantum dot marking test strip and method for synchronously and quantitatively detecting multiple indices of cardiac markers. A marking pad (3) of the test strip is coated by a mixture of all cardiac marker antibodies correspondingly marked by quantum dots with different wavelengths; a zone T (4) of an analysis membrane (7) is coated by a mixture of all cardiac marker antibodies; and a zone C (5) of the analysis membrane (7) is coated by a second antibody. The standard curve of each detected matter is stored by using an electronic tag and is mounted on the test strip. According to the test strip, the standard curve data stored by the electronic tag is read by a detector with a signal detection function, and the concentrations of all the cardiac markers in a sample are synchronously and quantitatively detected by combining fluorescence intensity corresponding to a to-be-detected sample detected by the detector.

The invention relates to ex-situ biosensors that impedimetrically detect cardiac biomarkers of interest in a bodily fluid sample derived from a patient. The biosensors include a multi-array of vertically aligned platinum wires having immobilized thereon an aptamer that is selected to specifically and selectively bind to the cardiac markers of interest. The biosensors are contacted with a portion of the bodily fluid sample, and the aptamer binds to the cardiac markers of interest in the bodily fluid sample. As a result, an electrochemical impedance signal is generated and therefore, a change in electrochemical impedance is indicative of the presence of the cardiac markers of interest in the bodily fluid sample. The biosensors are point-of-care, on-demand devices that can be used in a medical environment, as well as in domestic settings.

A method for diagnosis of heart failure in a human subject, which comprises the step of: measuring an endoplasmic reticulum (ER)-initiated apoptotic signal such as CHOP in a biological sample originated from the subject is disclosed. According to the invention, by employing the new marker in stead of or in addition to a conventional cardiac marker such as BNP, more accurate diagnosis of heart failure can be attained.

The invention discloses a method of combining salvianolic acid B to induce directed myocardiac differentiation of iPSCs by a sodium-calcium exchanger 1 promoter and belongs to the technical field of medicines. The method comprises the following steps: constructing NCX1 lentivirus particles and transfecting the NCX1 lentivirus particles to iPSCs; and performing assistance with combined induction of salvianolic acid B. The concentration of the salvianolic acid B is 1-100[mu]M. The method disclosed by the invention verifies that the sodium-calcium exchanger 1 promoter combined with the salvianolic acid B can effectively induce directed myocardiac differentiation of iPSCs. The salvianolic acid B can effectively promote NCX1 expresion and promote remarkable improvement of expressions of cardiac markers alpha-actin and cardiac troponin (cTnT). According to the shape of the iPSCs, fusiform change of a myocardial cell sample can be seen. IPSCs as a seed cell for repairing acute myocardial infarction have a wide application prospect.

The invention discloses a preparation method of a fluorescence immunochromatographic detection kit for composite quantitative detection of hypersensitive C-reactive protein (hs-CRP), myeloperoxidase (MPO), lipoprotein phospholipase A2 (Lp-PLA2), oxidized low-density lipoprotein (ox-LDL) and F2-isoprostaglandin (F2-isop). The kit comprises a desiccant, a sealing bag and a detection card. The detection card comprises a pentaplet buckle card and a reagent strip, wherein the reagent strip comprises a sample pad, a combination pad, a blood filtering membrane, a nitrocellulose membrane, absorbent paper and a PVC bottom plate; the combination pad is coated with an hs-CRP antibody, an MPO antibody, an Lp-PLA2 antibody, an ox-LDL antibody and an F2-isop antibody; and the antibody is one or more pairing combinations of a monoclonal antibody, a polyclonal antibody, an antibody fragment or a chimeric antibody. The preparation method disclosed by the invention has the outstanding advantages of specificity, sensitivity, high yield, rapidness, simplicity, convenience, good repeatability, easiness in automation, minimization of false positive, high detection efficiency, low detection cost and thelike, and has important practical significance.

The invention discloses a method for detecting an acute myocardial infarction marker cTnI (cardiac troponin I) based on CRISPR (clustered regularly interspaced short palindromic repeats) Cas13d gene editing protein. A sample is incubated on an elisa plate to form an antibody-cTnI compound, two ssDNAs are annealed to form a double-chain cTnI DNA aptamer with a T7 promoter sequence, and the aptamer and the cTnI-antibody compound are incubated and combined to form an antibody-cTnI-aptamer ternary sandwich structure. The structure can be recognized by T7 RNA polymerase, transcripts and expresses activator RNA, and can be recognized by a CRISPR Cas13d-crRNA compound, and the side cutting activity generated by activation can effectively cut RNA fluorescent reporter molecules to generate certain fluorescent signals. The Cas13d gene editing protein is applied to accurate detection of the cardiac marker cTnI for the first time.

The assisted capacity device calculator for myocardial infarction system is intended to assist a user during, or recently having had, a myocardial infarction and is intended to aid to help all people, especially those who have are susceptible to having myocardial infarctions, i.e., heart attacks. The apparatuses of the system are predominately mobile but can also be stationary and can be programmed by receiving and selecting pre-set commands to operate and assist a user but is particularly adapted for use when a user is having symptoms of a myocardial infarction. Particularly, the system is capable of prompting the user to respond to myocardial infarction symptoms, by answering various symptom questions, and if necessary, activate electrocardiographic (ECG) components, to test for electro cardio activity and/or a blood analyzer sensors to test for cardiac markers. The apparatuses of the also are provided with various features including an illuminated display panel, a CPS tracking capability, alarms among other things.

The invention is suitable for the field of cardiovascular and cerebrovascular disease early diagnosis, and provides a digitized cardiovascular and cerebrovascular disease early diagnosis system. The digitized cardiovascular and cerebrovascular disease early diagnosis system comprises a diagnosis and detection module, an information management module and an auxiliary module, wherein joint inspection and dynamic detection are carried out on an electrocardiogram and a cardiac marker by the diagnosis and detection module, so that the accuracy and timeliness of the cardiovascular and cerebrovascular disease early diagnosis can be effectively promoted; a 12-lead electrocardiograph is provided with a first communication unit, so that remote expert consultation is realized, and the accuracy rate of diagnosis is improved; and the information management module is provided with a second communication unit, so that remote wireless communication of the digitized system and a hospital information system, as well as the digitized system and a laboratory information management system is realized, and the informationization degree of the digitized system is improved. The digitized cardiovascular and cerebrovascular disease early diagnosis system is portable in design, strong in maneuverability, convenient to fold and unfold, can be used at any time, simple to operate and complete in detection items, is suitable for rapidly diagnosing on site, and is capable of assisting medical workers to treat pointing to symptoms, so that the mortality rate of patients is lowered.