Fluorescent microsphere combined detection device for cardiac marker and preparation method of fluorescent microsphere combined detection device
A technology of fluorescent microspheres and combined detection, which is applied in the medical field, can solve the problems of easy quenching of fluorescent antibodies, insufficient amount of adsorbed protein on NC membrane, weak binding force, etc., achieve good biocompatibility, and improve coating efficiency , the effect of high mechanical strength
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0043] Example 1 A fluorescent microsphere combined detection device for cardiac markers
[0044] The sample pad 1, the immunofluorescence antibody glass fiber membrane 2, the nitrocellulose membrane 3, and the absorption pad 4 are pasted on the plastic plate 5 respectively, and the two ends of the nitrocellulose membrane 3 are connected with the absorption pad 4, the immunofluorescence antibody glass fiber respectively. The membrane 2 is overlapped, and the other end of the immunofluorescence antibody glass fiber membrane 2 is overlapped with the sample pad 1; the nitrocellulose membrane 3 is provided with a first detection line T1, a second detection line T2, and a third detection line T3 , and quality control line C; the solid phase of the first detection line T1 has a highly specific MPO antibody, the solid phase of the second detection line T2 has a high specificity cTnI antibody, and the third detection line There is a highly specific NT-proBNP antibody on the solid phas...
Embodiment 2
[0045] Example 2 A fluorescent microsphere combined detection device for cardiac markers
[0046] On the basis of Example 1, a fourth detection line T4 is further set on the nitrocellulose membrane 3, and the solid phase of the fourth detection line T4 has a highly specific D-dimer antibody.
Embodiment 3
[0048] The preparation method of the fluorescent microsphere combined detection device for cardiac markers in the above-mentioned embodiment 1
[0049] Include the following steps:
[0050](a) Preparation of immunofluorescent microspheres, take 100ul of microsphere suspension with a solid content of 1%, dilute it 10 times with ultrapure water, that is, 1000ul, add it to a test tube, and take 40ul of N-hydroxysuccinimide solution Add (NHS) to the microsphere suspension and mix well, then add 40ul of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) to the microsphere suspension Mix well in the medium, and react at room temperature for 0.5 hours; ultrasonically sonicate the reacted suspension to resuspend the microspheres on the tube wall in the aqueous solution, then centrifuge the microsphere suspension at 8000r / min, 15min, pour Remove the supernatant, add 1ml ultrapure water, and then disperse evenly with ultrasonic waves;
[0051] (b) Immunofluorescent micr...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com