Fluorescent microsphere combined detection device for cardiac marker and preparation method of fluorescent microsphere combined detection device

A technology of fluorescent microspheres and combined detection, which is applied in the medical field, can solve the problems of easy quenching of fluorescent antibodies, insufficient amount of adsorbed protein on NC membrane, weak binding force, etc., achieve good biocompatibility, and improve coating efficiency , the effect of high mechanical strength
CN109799334AInactive Publication Date: 2019-05-24JILIN UNIV +1
9 Cites 9 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JILIN UNIV
Publication Date
2019-05-24
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a fluorescent microsphere combined detection device for a cardiac marker and a preparation method of the fluorescent microsphere combined detection device, and belongs to thefield of medical detection equipment. A sample pad, an immunofluorescent antibody glass fiber membrane, a nitrocellulose membrane and an absorption pad respectively adhere to a plastic plate, the twoends of the nitrocellulose membrane are respectively overlapped with the absorption pad and the immunofluorescent antibody glass fiber membrane, the other end of the immunofluorescent antibody glass fiber membrane is overlapped with the sample pad, the nitrocellulose membrane is provided with a first detection line T1 with a solid-phase high-specificity MPO antibody, a second detection line T2 with a solid-phase high-specificity cTnI antibody, a third detection line T3 with a solid-phase high-specificity NT-proBNP antibody, a fourth detection line T4 with a solid-phase high-specificity D dimerantibody and a quality control line C; and a goat anti-mouse IgG polyclonal antibody is sprayed on the quality control line C. The fluorescent microsphere combined detection device for the cardiac marker has the advantages that the sensitivity and the specificity of reaction are effectively improved, the use amount of immune microspheres can further be reduced under the same threshold value, thecost is saved, and the device is simple and convenient to operate and high in practicability.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to the field of medical detection equipment, in particular to a combined detection device for cardiac markers and a preparation method thereof, which uses fluorescence immunochromatography technology and the principle of double-antibody sandwich method to quantitatively detect human myeloperoxidase (MPO) in plasma of clinical specimens. ), cardiac troponin I (cTnI), N-terminal pro-brain natriuretic peptide (NT-proBNP) and D-dimer detection device and preparation method thereof, which can realize sensitive, specific and rapid detection of cardiac markers. Background technique

[0002] Cardiopulmonary function indicators are an important reference for clinical diagnosis and disease prevention. Monitoring cardiopulmonary function is of great value in the diagnosis, treatment and prognosis of patients with chronic cardiopulmonary diseases. The detection of cardiopulmonary function indicators is widely used in clinical emergenc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More