Kit for combined quantitative detection of five cardiac markers and preparation method thereof

A combined detection and kit technology, applied in the field of clinical medical testing, can solve the problems of inability to achieve risk assessment, low detection efficiency, waste of consumables, etc., and achieve high yield, easy automation, and obvious advantages

Pending Publication Date: 2020-10-30
CHANGZHOU BIOWIN BIOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese invention patent CN201810746660.2 discloses a joint quantitative detection of inflammatory markers serum amyloid A, high-sensitivity C-reactive protein and procalcitonin detection test paper. This method only involves inflammatory markers. will be detected, risk assessment cannot be achieved
At present, the single-indicator detection products for clinical application can only detect one indicator at the same time, and five indicators need five detection reagents to complete, which has defects such as low detection efficiency, high cost, waste of consumables, etc.

Method used

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  • Kit for combined quantitative detection of five cardiac markers and preparation method thereof
  • Kit for combined quantitative detection of five cardiac markers and preparation method thereof
  • Kit for combined quantitative detection of five cardiac markers and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of sample pad

[0045] Dissolve BSA and NaCl with pH 8.0 sodium bicarbonate buffer until the final concentration of BSA is 2% and the final concentration of NaCl is 0.1M, then add the surfactant Tween20 to the final concentration of 0.5%, adjust the pH to 8.0, press the glass fiber The water absorption capacity of the plain film is 60uL / cm2, and the above buffer solution is evenly spread on the glass cellulose film, and dried at 37°C for 8 hours to obtain a sample pad. Store at 4°C for later use.

[0046] (2) Preparation of bonding pad

[0047] The monoclonal antibody labeled with fluorescent microspheres is evenly spread on the sample pad prepared in step (1), freeze-dried in a vacuum, sealed, and stored at room temperature for use. The preparation process of the monoclonal antibody labeled with fluorescent microspheres is as follows:

[0048] a. Preparation of hs-CRP monoclonal antibody labeled with fluorescent microspheres

[0049] Take 0.5 mL of the fluores...

Embodiment 2

[0068] Detection of hypersensitivity C-reactive protein, myeloperoxidase, lipoprotein phospholipase A2, oxidized low-density lipoprotein and F2-isoprostaglandin fluorescence immunochromatographic detection kit.

[0069] (1) Draw a standard curve

[0070] Different concentrations of hypersensitive C-reactive protein, myeloperoxidase, lipoprotein phospholipase A2, oxidized low-density lipoprotein, and F2-isoprostaglandin antigen standards were added to the sample pads of the kit prepared according to Example 1. With 7 different concentrations, the standards of hypersensitive C-reactive protein antigen are 0, 0.5, 5, 10, 40, 100, 150 ng / mL, and the standards of myeloperoxidase antigen are 0, 10, 50, 100, 200, 500, 1000 ng / mL, lipoprotein phospholipase A2 antigen standards were 0, 50, 100, 200, 500, 1000 and 1500 ng / mL, oxidized low density lipoprotein antigen standards were 0, 0.5, 1, 2, 4, 8, 16 and 20μg / mL, the standard F2-isoprostaglandin antigen is 0, 30, 50, 200, 400, 800 and 15...

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Abstract

The invention discloses a preparation method of a fluorescence immunochromatographic detection kit for composite quantitative detection of hypersensitive C-reactive protein (hs-CRP), myeloperoxidase (MPO), lipoprotein phospholipase A2 (Lp-PLA2), oxidized low-density lipoprotein (ox-LDL) and F2-isoprostaglandin (F2-isop). The kit comprises a desiccant, a sealing bag and a detection card. The detection card comprises a pentaplet buckle card and a reagent strip, wherein the reagent strip comprises a sample pad, a combination pad, a blood filtering membrane, a nitrocellulose membrane, absorbent paper and a PVC bottom plate; the combination pad is coated with an hs-CRP antibody, an MPO antibody, an Lp-PLA2 antibody, an ox-LDL antibody and an F2-isop antibody; and the antibody is one or more pairing combinations of a monoclonal antibody, a polyclonal antibody, an antibody fragment or a chimeric antibody. The preparation method disclosed by the invention has the outstanding advantages of specificity, sensitivity, high yield, rapidness, simplicity, convenience, good repeatability, easiness in automation, minimization of false positive, high detection efficiency, low detection cost and thelike, and has important practical significance.

Description

Technical field [0001] The invention relates to the field of clinical medical testing, in particular to a combined quantitative detection of hypersensitive C-reactive protein, myeloperoxidase lipoprotein phospholipase A2, oxidized low-density lipoprotein and F2-isoprostaglandin fluorescence immunochromatographic detection Kit and preparation method thereof. Background technique [0002] Cardiovascular disease is a common clinical emergency and critical illness, with a high fatality rate and a dangerous condition, which seriously threatens human health and life. Some data indicate that the basic pathological change of cardiovascular disease is atherosclerosis. Recent studies have shown that inflammatory response is the basis of atherosclerosis and cardiovascular disease. In particular, chronic inflammation is believed to increase the risk of arterial injury disease. Atherosclerotic plaque is easy to rupture and plays a key role in the formation of atherosclerosis. Arterial injur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/533
CPCG01N33/5304G01N33/533
Inventor 刘冰章燕周伟李亚楠刘凤鸣
Owner CHANGZHOU BIOWIN BIOPHARM
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