Hybridoma cell strain capable of secreting monoclonal antibody with resistance to maize chlorotic mottle virus and application of monoclonal antibody

A technology of chlorotic mottle virus and hybridoma cell line, which is applied in antiviral immunoglobulin, analytical materials, biochemical equipment and methods, etc., and can solve problems such as low efficiency

Pending Publication Date: 2015-03-25
ZHEJIANG UNIV
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Problems solved by technology

[0004] In order to grasp the incidence and hazards of maize chlorotic mottle virus (MCMV) disease in my country, strengthen the early monitoring and early warning of maize chlorotic mottle virus disease in my country, and scientifically guide the prevention and control, it is urgent to establis

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  • Hybridoma cell strain capable of secreting monoclonal antibody with resistance to maize chlorotic mottle virus and application of monoclonal antibody

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Embodiment Construction

[0012] The hybridoma cell line 24F6 secreting monoclonal antibody against corn chlorotic mottle virus was preserved on July 3, 2014 in the General Microbiology Center of China Microbiological Culture Collection Management Committee, Institute of Microbiology, Chinese Academy of Sciences, address: Beichen West Road, Chaoyang District, Beijing No. 1 Yard No. 3, Zip Code: 100101, and the preservation number is CGMCC No. 9339. It can secrete monoclonal antibodies against corn chlorotic mottle virus.

[0013] Anti-maize chlorotic mottle virus monoclonal antibody ascites indirect ELISA titer up to 10 -8 , the antibody type and subclass are IgG1, kappa chain, the monoclonal antibody can have a specific immunological binding reaction with the capsid protein of maize chlorotic mottle virus, and can detect maize chlorotic mottle virus in infected seeds and aphids Green mottle virus.

[0014] Monoclonal antibodies against maize chlorotic mottle virus have specific immune reactions only ...

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Abstract

The invention discloses a hybridoma cell strain capable of secreting a monoclonal antibody with resistance to maize chlorotic mottle virus and an application of the monoclonal antibody. The hybridoma cell strain capable of stably propagating and secreting a monoclonal antibody with resistance to MCMV (Maize Chlorotic Mottle Virus) can be prepared by taking virus particles of purified maize chlorotic mottle virus (MCMV) as antigen immune BALB/c mice, carrying out cell fusion, screening and cloning; the preservation number of the hybridoma cell strain is CGMCC No.9339; the monoclonal antibody secreted by the hybridoma cell strain has ascites indirect ELISA valence which reaches 10<-8>; the type and subclass of the antibody is IgG1; a chain is a kappa chain. The monoclonal antibody only has specific reactivity with MCMV; an immunological detection method is built by 24F6 monoclonal antibody and is used for detecting MCMV in virus-transferring amboceptor thrips, maize plates and seeds; the hybridoma cell strain 24F6 and the monoclonal antibody are acquired and a relevant serological detection method is built, so that the technical support is provided for the quarantine, detection, prediction and scientific prevention and control of the virus disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell strain secreting monoclonal antibody against corn chlorotic mottle virus and the application of the monoclonal antibody. Background technique [0002] Maize chlorotic mottle virus (MCMV) belongs to Tombusviridae Machlomovirus genus and is an important virus that harms corn. MCMV was first discovered in Peru in 1974. The symptoms of the plant are as follows: the leaves gradually lose chlorosis, turn yellow, and the whole leaf shows yellow and green mottled stripes, so it is named corn chlorotic mottle virus. After the host is infected, the symptoms are mottled necrosis, curling, leaf tip necrosis, dwarfing, plant death, etc. Natural infection of crops results in a loss of 10% to 15%, and the loss of experimental corn reaches 59%. Mosaic virus (SCMV) co-infection losses were as high as 100%. The virus has been included in my country's newly revised list of harmful o...

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Application Information

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IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569C12R1/91
Inventor 吴建祥周雪平
Owner ZHEJIANG UNIV
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