Hybridoma cell strain secreting monoclonal antibody against barley yellow dwarf virus GAV strain and application of monoclonal antibody thereof

Abstract

The invention discloses a hybridoma cell strain secreting a monoclonal antibody against a barley yellow dwarf virus GAV strain, and an application of the monoclonal antibody thereof. A BALB / c mouse is immunized with barley yellow dwarf virus (BYDV) GAV strain virions which is used as antigens and is purified by a differential centrifugation method, and a hybridoma cell strain 27E1 which can be subcultured stably and secretes a monoclonal antibody against BYDV GAV is obtained through cell fusion, screening, and cloning, and the accession number is CGMCC No. 8781. The monoclonal antibody secreted by the hybridoma cell has an ascetic ELISA titer of more than 10<-6>, and the antibody type and subtype are IgG1, kappa chain. A specific reaction can be carried out between the monoclonal antibody secreted by the hybridoma cell strain 27E1 and the barley yellow dwarf virus GAV strain, but the monoclonal antibody does not react with rice stripe virus, rice ragged stunt virus, Chinese wheat mosaic virus, wheat yellow mosaic virus, barley yellow mosaic virus, barley yellow dwarf virus GPV strains and PAV strains. The hybridoma cell strain 27E1 and the secreted monoclonal antibody provide technical and material support for the diagnosis, detection and scientific prevention and control of wheat viral diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-barley yellow dwarf virus GAV strain monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Wheat yellow dwarf disease is caused by barley yellow dwarf virus (BYDV), which is distributed all over the world. It has a wide range of hosts and can parasitize more than 150 kinds of monocot plants, especially barley, Wheat, oats, rice and other cereal crops. First discovered on barley in California, USA in 1950, it is one of the most serious and widespread plant viruses in the world. It was discovered in my country in 1960 on wheat in Shaanxi and Gansu. It is the most important viral disease of wheat. It is called the "yellow plague" of wheat and "cancer of wheat". At present, it is mainly distributed in winter wheat areas, spring wheat areas and winter-spring wheat mixed planting areas in Northwest China, No...

AI Technical Summary

Problems solved by technology

All of these methods have their limitations and are not suitable for the detection of large quantities of field samples

Serological methods are suitable for batch detection of field samples, but must rely on specific virus monoclonal antibodies

At present, no specific monoclonal antibody against this virus has been developed at home and abroad.

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