Hybridoma cell strain secreting monoclonal antibody against banana bunchy top virus and application of monoclonal antibody thereof

Abstract

The invention discloses a hybridoma cell strain secreting a monoclonal antibody against banana bunchy top virus, and an application of the monoclonal antibody thereof. A BALB / c mouse is immunized with purified banana bunchy top virus (BBTV) virions as antigens, and a hybridoma cell strain 22E3 which can be subcultured stably and secretes a monoclonal antibody against BBTV is obtained through cell fusion, screening, and cloning, and the accession number is CGMCC No. 8780. The 22E3 monoclonal antibody has an ascetic indirect ELISA titer of up to 10<-7>, and the antibody type and subtype are IgG1, kappa chain. Specific reaction can be carried out between the monoclonal antibody and BBTV. A dot-ELISA method for detecting BBTV in bananas established with the 22F3 monoclonal antibody can detect the virus even when a diseased leaf is diluted by 1:320 times (w / v, g / mL). The acquisition of the hybridoma cell secreting monoclonal antibody against BBTV and its monoclonal antibody, and the establishment of a related serological detection method provide technical and material support for the diagnosis, prediction and scientific prevention and control of the viral disease.

Description

Technical field [0001] The present invention involves the field of biotechnology, especially the application of hybrid tumor cells and monoclonal antibodies that secrete the banana beam -top virus monoclonal antibody. Background technique [0002] The pathogen of banana beam is the banana bunchy Top Virus (BBTV).The disease was first discovered in Fiji in 1889, and then in Australia, India, Pakistan, Indonesia, Mainland China and Taiwan, Gabon, Pacific islands, Egypt, Congo, the Philippines, Vietnam and other countries and regions.In the 20th century, Banana beams became one of the important devastating diseases on bananas. The disease threatened about the production of about 1 / 4 of the world's banana production areas in Asia, Africa and South Pacific.In China, as early as 1900, it was recorded in BBTV. By the 1950s and 1990s, banana beams were popular in major banana production areas such as Fujian, Guangdong, Guangxi, Yunnan, and Hainan, causing major economic losses.It affecte...

AI Technical Summary

Problems solved by technology

Infected plants at the seedling stage are mainly dwarfed, and the young leaves become smaller and narrower, clustered in fascicles, and dark green dotted and linear "blue tendons" first appear on the veins; the young leaves of infected plants at the middle seedling stage are yellowish-white at the beginning. Then gradually darken to dark stripes and extend to the main vein; when infected at the late stage of booting, the newly sprouted leaves lose chlorosis and become brittle, and the heading stagnates; when infected at the early ear stage, the diseased plant takes the shape of mosaic leaves, and the cob no longer bends down. Bananas stop growing; when infected at the late heading stage, bananas also stagnate, and the root system of the diseased plant grows poorly or rots. If the fruit is damaged, the shape of the fruit will become thinner, the fruit flavor will become lighter, and the commodity value will be lost.

In order to grasp the incidence of banana bunchy top virus, strengthen the early monitoring and early warning of banana bunchy top virus in my country, and scientifically guide the prevention and control, it is urgent to establish a high-throughput detection method for BBTV in Musa, but there is no such high-throughput detection at present method, only low-efficiency electron microscope observation, PCR method, nucleic acid electrophoresis and other methods are used for small sample detection

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