Hybridoma cell strain secreting monoclonal antibody against watermelon mosaic virus and application of monoclonal antibody

Abstract

The invention discloses a hybridoma cell strain secreting monoclonal antibody against watermelon mosaic virus and application of the monoclonal antibody. Purified virus particles of watermelon mosaic virus (WMV) are used as an antigen to immunize BALB / c mice; through cell fusion, screening and cloning, one strain of hybridoma cell strain 1C5 capable of stable passage and secreting anti-WMV monoclonal antibodies is obtained; and the strain has a preservation number CGMCC No.9340. The monoclonal antibody secreted from the hybridoma cell strain has ascites indirect ELISA titer of 10 <-7>, and the antibody type and subtype are IgG1, kappa chain. The monoclonal antibody has specificity reaction with CMV. The 1C5 antibody is used to establish a dot-ELISA virus method for detection of WMV in aphid and in white pumpkin. The hybridoma cell strain 1C5, monoclonal antibody thereof and the establishment of the related serological detection method provide technical and material support for the diagnosis, prediction and scientific prevention and control of the virus.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell strain secreting anti-watermelon mosaic virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Watermelon mosaic virus (WMV) is an important member of Potyvirus, which is transmitted non-persistently by aphids. WMV is distributed all over the world, mainly in temperate and Mediterranean regions. In 1954, Anderson and others in the United States first discovered the virus on watermelons in Florida. In 1965, R.E.Webb and H.A.Scot were the first to isolate two viruses that harm watermelon from watermelon, and they were divided into two strains according to the difference in host range and the presence or absence of interactive protection, which were named WMV-1 and WMV-1 respectively. WMV-2. Later, Purcifull found in his research that WMV-1 is actually a strain of PRSV. The virus that occurs in large quantities on wate...

AI Technical Summary

Problems solved by technology

The current early warning and monitoring system for watermelon mosaic virus disease is not perfect, and even many major endemic areas have not carried out relevant prediction and prevention and control work

In addition, currently only field observation, RT-PCR detection, electron microscope observation and other methods are used to detect the virus. These methods are inefficient and not suitable for large-scale sample detection

The serological method is simple to operate, has good specificity, and can process a large number of samples at the same time, but it must rely on specific virus monoclonal antibodies

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