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Parathyroid hormone kit and preparation method thereof

A parathyroid hormone and kit technology, applied in the field of immunodiagnosis, can solve the problems of unstable test results, low accuracy, complicated operation, etc., and achieve the effects of improving specificity, detection speed, low cost, and simple operation

Inactive Publication Date: 2019-01-15
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems of the method of parathyroid hormone in the prior art, such as low accuracy, short delivery period, complicated operation, unstable test results, and high price, and provide a parathyroid hormone Kit and preparation method thereof

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  • Parathyroid hormone kit and preparation method thereof
  • Parathyroid hormone kit and preparation method thereof
  • Parathyroid hormone kit and preparation method thereof

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preparation example Construction

[0050] The present invention also provides the preparation method of above-mentioned parathyroid hormone test kit, comprises the following steps:

[0051] Step 1, preparation of R1 reagent

[0052] Add the streptavidin magnetic particle solution into the TBST solution, mix well, place on the magnetic separator until the supernatant is free of turbidity, discard the supernatant, keep the magnetic particles, wash the magnetic particles with buffer Ⅰ, Then use buffer I to make a solid-phase reagent with a magnetic particle concentration of 0.007% to 1%, which is the R1 reagent;

[0053] Among them, the ratio of streptavidin magnetic particles to TBST solution is preferably 50mg:10mL; general cleaning needs to be repeated three times; R1 reagent needs to be stored at 2-8°C.

[0054] Step 2, preparation of R2 reagent

[0055] First put the parathyroid hormone monoclonal antibody into the centrifuge tube to ensure that the parathyroid hormone monoclonal antibody is at the bottom o...

Embodiment 1

[0071] The preparation method of parathyroid hormone test kit comprises the steps:

[0072] Preparation of R1 reagent: Take 0.5 ml (50 mg) of a solution of streptavidin magnetic particles (particle size 0.1 μm) with a concentration of 100 mg / ml and add it to the TBST solution, mix well, and place it on a magnetic separator until the supernatant If there is no turbidity, discard the supernatant, keep the magnetic particles, and wash them three times with buffer I. After washing, use buffer I to make a solid-phase reagent with a magnetic particle concentration of 0.05%, which is the R1 reagent, and store at 2-8°C. ;

[0073] Preparation of R2 reagent: Put 500 μg of mouse parathyroid hormone monoclonal antibody into a centrifuge tube to ensure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 20 seconds), then add carbonic acid buffer solution, mix well, and then add 5 μl 2mg / mL DMF solution of acridinium ester, centrifuge at room temp...

Embodiment 2

[0077] The preparation method of parathyroid hormone test kit comprises the steps:

[0078] Preparation of R1 reagent: Take 0.5 ml (50 mg) of a solution of streptavidin magnetic particles (particle size 0.5 μm) with a concentration of 100 mg / ml and add it to the TBST solution, mix well, and place it on a magnetic separator until the supernatant If there is no turbidity, discard the supernatant, keep the magnetic particles, and wash them three times with buffer I. After washing, use buffer I to make a solid-phase reagent with a magnetic particle concentration of 1%, which is the R1 reagent, and store at 2-8°C. ;

[0079] Preparation of R2 reagent: Put 500 μg of mouse parathyroid hormone monoclonal antibody into a centrifuge tube to ensure that the antibody is at the bottom of the centrifuge tube (centrifuge at room temperature for 20 seconds), then add carbonic acid buffer solution, mix well, and then add 5 μl 2mg / mL DMF solution of acridinium ester, centrifuge at room tempera...

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Abstract

The invention relates to a parathyroid hormone kit and a preparation method thereof, belongs to the technical field of immunologic diagnosis, and solves the technical problems that a parathyroid hormone method in the prior art is low in accuracy, short in shelf life, complicated in operation, unstable in test result and high in cost. The kit disclosed by the invention comprises a reagent R1, a reagent R2 and a reagent R3; the reagent R1 is a buffer solution I containing streptavidin magnetic particles; the reagent R2 is a parathyroid hormone monoclonal antibody solution labeled with a chemiluminescent marker diluted by the buffer solution I; and the reagent R3 is a coupled labeled parathyroid hormone monoclonal antibody solution diluted by the buffer solution I. The kit detects parathyroidhormone by adopting a chemiluminescence immunoassay, and has the advantages of high detection sensitivity, accurate quantitative detection, simple operation, no radioactive risk, short detection timeand capability of detecting antigen and antibody type target substances.

Description

technical field [0001] The invention belongs to the technical field of immunodiagnosis, and in particular relates to a parathyroid hormone kit and a preparation method thereof. Background technique [0002] Parathyroid hormone (parathyoidhormone, PTH) is synthesized in the parathyroid gland and secreted into the blood. PTH(1-84), also known as biointegrity PTH, is a single-chain polypeptide containing 84 amino acids with a molecular weight of about 9500 Daltons. PTH has a half-life of only a few minutes before being broken into various fragments and cleared from the blood circulation at an extremely rapid rate. The secretory activity of the parathyroid gland can be directly understood by detecting the biological whole segment of PTH (1-84). [0003] The main effect of PTH is to increase blood calcium, which is mainly achieved by promoting the release of calcium in the bone and the renal reabsorption in the distal tubule. The interaction of PTH and calcitonin ensures the s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/58G01N21/76G01N33/531G01N33/532
Inventor 高智玲李冬梅高威孙成艳何浩会
Owner DIRUI MEDICAL TECH CO LTD
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