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Osteochondral graft preservation liquid and preparation method thereof

A technology for osteochondral grafts, applied in the field of osteochondral graft preservation solution and its preparation, can solve the problems of short storage time in vitro and low survival rate of chondrocytes, achieve good biological activity, improve preservation effect, and prolong storage the effect of time

Active Publication Date: 2015-09-30
TAISHAN MEDICAL UNIV
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Problems solved by technology

[0007] The object of the present invention is to provide an osteochondral graft preservation solution and its preparation method, aiming to solve the existing problems of continuously maintaining the activity of osteochondral grafts, low survival rate of chondrocytes, and short storage time in vitro

Method used

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  • Osteochondral graft preservation liquid and preparation method thereof

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preparation example Construction

[0027] Such as figure 1 As shown, the preparation method of the osteochondral graft preservation solution of the embodiment of the present invention comprises the following steps:

[0028] S101: Take glucose, amino acids, antioxidants, basic fibroblast growth factor, inorganic salts, vitamins, sodium pyruvate, penicillin and deionized water;

[0029] S102: Prepare the raw materials of S101 into a solution, stir evenly, adjust the pH value to 7.30-7.50, and filter to sterilize;

[0030] S103: washing the fresh allogeneic osteochondral graft with sterile saline for 3-5 times, and soaking it in the prepared osteochondral graft preservation solution;

[0031] S104: Store the S103 osteochondral graft preservation solution at 4°C.

Embodiment 1

[0034] Embodiment 1: every 1000ml osteochondral graft preservation solution contains glucose 80mmol, amino acid 2.0mmol, antioxidant 0.5mmol, basic fibroblast growth factor (bFGF) 60nmol, inorganic salt 1mmol, vitamin 6mmol, sodium pyruvate 2mmol, penicillin 70U. The remainder is deionized water, and the pH value of the osteochondral preservation solution is 7.30-7.50. It is stored at 4°C. On the 21st and 35th day of storage, the chondrocyte survival rate of the osteochondral tissue was detected. On the 21st day of storage, the cell survival rate was 85%.

Embodiment 2

[0035] Embodiment 2: every 1000ml osteochondral graft preservation solution contains glucose 100mmol, amino acid 0.1mmol, antioxidant 0.2mmol, basic fibroblast growth factor (bFGF) 25nmol, inorganic salt 20mmol, vitamin 9mmol, sodium pyruvate 1mmol, penicillin 60U. The remainder is deionized water, and the pH value of the osteochondral preservation solution is 7.30-7.50. It is stored at 4°C. On the 21st and 35th day of storage, the chondrocyte survival rate of the osteochondral tissue was detected. On the 21st day of storage, the cell survival rate was 86%.

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Abstract

The invention discloses an osteochondral graft preservation liquid and a preparation method thereof. The osteochondral graft preservation liquid comprises the following raw materials: 80-125mmol of glucose, 0.1-2.0mmol of amino acid, 0.2-2.0mmol of an antioxidant, 5-60nmol of a basic fibroblast growth factor, 1-120mmol of inorganic salt, 1-9mmol of vitamin, 1-2mmol of sodium pyruvate, 50-70U of penicillin and the balance of deionized water. The method comprises the following steps: preparing a solution, adjusting the pH to be 7.30-7.50, filtering and degerming; cleaning fresh allogeneic osteochondral graft with a sterile saline solution for 3-5 times, and putting into the prepared osteochondral graft preservation liquid to soak; and storing at 4 DEG C. The osteochondral graft preservation liquid has the efficacies of resisting cartilage cells senescence in preservation, inhibiting cartilage cells apoptosis, keeping good biological activity of cartilage tissues, maintaining the survival rate of cartilage cells, prolonging the storage time and improving the storage effect of the osteochondral graft.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an osteochondral graft preservation solution and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards in our country and the comprehensive popularization of sports, the number of patients with articular cartilage injuries has increased significantly. At present, there is no ideal method for the repair and treatment of articular cartilage damage. Therefore, the method of repairing articular cartilage damage has become an international medical problem to be solved urgently. [0003] As articular cartilage lesions cannot be repaired naturally, without effective treatment, it will eventually lead to the occurrence of osteoarthritis, loss of joint function, and severe cases may cause disability. Artificial joint replacement is suitable for the treatment of elderly patients with osteoarthritis, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 亓建洪刘召庆宋洪强陈彬张延明谢地
Owner TAISHAN MEDICAL UNIV
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