Berberine-containing serum-free medium for mesenchymal stem cells
A serum-free culture medium and stem cell technology, applied in the field of stem cells, can solve problems such as unsatisfactory cell proliferation, prolong cell growth cycle, and short cell growth cycle, and achieve delayed entry into the decline period, good cell proliferation rate, and preparation methods simple effect
CN105112366AActive Publication Date: 2015-12-02广东美赛尔细胞生物科技有限公司
Patent Information
- Authority / Receiving Office
- CN · China
- Current Assignee / Owner
- 广东美赛尔细胞生物科技有限公司
- Publication Date
- 2015-12-02
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Abstract
The invention provides berberine-containing serum-free medium for mesenchymal stem cells, comprising a DMEM basic medium and further comprising, by concentration, 5-110mg/L berberine, 3-20mg/ml basic fibroblast growth factors, 3-20mg/ml epidermal growth factors, 1-10Mug/mL glutathione, 1-10mg/ml recombinant human insulin, 5-15mg/ml human serum albumin, 1-10Mug/mL transferrin, 5-15mg/L fibronectin, 5-30mg/L putrescine, 0.37-4mg/L aminoethanol, 10-50Mug/mL hydrocortisone, and 0.02-0.2mg/ml sodium pyruvate. The invention belongs to the technical field of stem cells; the medium has good cell adherence, high cell proliferation rate and capable of delaying decline phase of MSCs (mesenchymal stem cells) and prolong growth period of the cells.
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Claims
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