Method for evaluating ecotoxicological impact of microplastics

Abstract

The invention discloses a method for evaluating the ecotoxicological impact of microplastics. The method includes the following steps that (1) artemia exposure experiment is performed; (2) intestinaltract collecting is performed; (3) DNA extraction and PCR amplification are performed; (4) Illumina Miseq sequencing is performed; (5) data processing is performed; (6) data analysis is performed, specifically, cluster analysis of the processed data finds that the main phylums in each group at the phylum level are Actinobacteria and Proteobacteria; and (7) ecotoxicology evaluation is performed, specifically, the microplastics have a certain toxic effect on organisms. The method is simple in operation, high in accuracy and short in experimental period, and can rapidly detect intestinal microorganisms. The method can quickly and accurately evaluate the ecotoxicological impact of the microplastics.

Description

technical field

[0001] The invention relates to the technical field of high-throughput sequencing, and relates to a method for evaluating the ecotoxicological impact of microplastics. Background technique

[0002] Microplastics (MPs) usually refer to plastic fragments, particles or fibers with a diameter or length of less than 5 mm, mainly derived from leaked raw materials in the plastic industry, debris formed in the ocean by large plastic waste, and plastics in various daily necessities. Microbead additives, etc. Due to the special properties of MPs, such as larger specific surface area, ability to absorb more pollutants, and easier entry into organisms, the impact on the marine environment is far greater than that of large plastic garbage. Due to the small particle size, MPs are easily absorbed by marine organisms. ingested and passed through the food chain. At present, the pollution status and ecological risks of MPs have attracted great attention from scholars at home...

Images