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Method for improving gas production rate by adding sodium propionate to domesticate microbial community of anaerobic fermentation system and application of method

A microbial community and anaerobic fermentation technology, applied in the field of environmental protection, can solve the problems of secondary pollution of the atmosphere and soil, threats to the ecological environment and human activities, etc. The effect of improving methane production capacity

Pending Publication Date: 2022-08-05
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After the biogas slurry and residue without reasonable harmless treatment enter the environment, it will directly bring secondary pollution to the water body, the atmosphere and the soil, posing a serious threat to the ecological environment and human activities.

Method used

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  • Method for improving gas production rate by adding sodium propionate to domesticate microbial community of anaerobic fermentation system and application of method
  • Method for improving gas production rate by adding sodium propionate to domesticate microbial community of anaerobic fermentation system and application of method
  • Method for improving gas production rate by adding sodium propionate to domesticate microbial community of anaerobic fermentation system and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for improving gas production by adding sodium propionate to domesticate microbial communities in an anaerobic fermentation system, comprising the following steps:

[0038] Step 1) Controlling the solid content of the biogas slurry and biogas residue mixture to 15%;

[0039] Step 2) Add 400 mL of culture medium to each liter of biogas slurry and biogas residue mixture treated in step 1), and after mixing evenly, take 700 mL of the mixture and put it into an anaerobic reactor with a volume of 1 L,

[0040] Step 3) Continue to add sodium propionate to the anaerobic digestion reactor of step 2), and the addition amount is 1g, 2g, 3g, 4g, 5g, 6g, 8g, 10g, 12g, 14g, 16g, 18g per liter respectively , 20g, 22g, 24g, 26g, 28g, 30g, 35g, 40g, 3 parallel for each concentration, pass nitrogen for ten minutes, cover for anaerobic digestion reaction;

[0041] The medium in described step 2) includes a large number of elements: NH 4 Cl 400mg / L, MgSO 4 ·6H 2 O 250mg / L, KCl...

Embodiment 2

[0047] On the 1st to 30th day of the anaerobic digestion reaction, the biogas meter (Geotech, Biogass5000, UK) was used to measure the anaerobic digestion reactors with sodium propionate added in each concentration in Example 1 and without sodium propionate added in Comparative Example 1. The amount of methane produced, the results are shown in Table 1, through counting analysis, make a curve, the results are as follows Figures 1 to 8 shown, where Figure 1 to Figure 4 For adding 0g / L, 1g / L, 2g / L, 3g / L, 4g / L, 5g / L, 6g / L, 8g / L, 10g / L, 12g / L, 14g / L, 16g / L , 18g / L, 20g / L, 22g / L, 24g / L, 26g / L, 28g / L, 30g / L, 35g / L, 40g / L sodium propionate daily output of methane with time change curve , Figures 5 to 8 For adding 0g / L, 1g / L, 2g / L, 3g / L, 4g / L, 5g / L, 6g / L, 8g / L, 10g / L, 12g / L, 14g / L, 16g / L , 18g / L, 20g / L, 22g / L, 24g / L, 26g / L, 28g / L, 30g / L, 35g / L, 40g / L of sodium propionate after the cumulative methane production curve graph .

[0048] Table 1 The amount of methane produced in th...

Embodiment 3

[0055] On the 0d, 7d, 10d, 15d, and 30d of the anaerobic digestion reaction, respectively, the samples were subjected to DNA extraction, PCR amplification, library construction, and high-throughput sequencing on the computer. According to the taxonomic analysis example The community structure at the bacterial phylum level in the anaerobic digestion reactors with sodium propionate at each concentration added in 1 and without sodium propionate added in Comparative Example 1, the results are shown in Table 2, and the relative abundance map of the bacterial phylum level is drawn, The result is as Figure 9 shown. Among them, C0-D0 means: C is the abbreviation of content concentration, 0 is 0mg / L, and D0 is the 0th day. Bacteria with relative abundance below 10% were classified as Other.

[0056] Table 2 Community structure (%) of bacterial phylum level in anaerobic digestion reactors with various concentrations of sodium propionate added

[0057]

[0058]

[0059] From Ta...

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Abstract

The invention provides a method for improving gas yield by adding sodium propionate to domesticate microbial communities of an anaerobic fermentation system and application of the method, and belongs to the technical field of environmental protection. According to the method, sodium propionate with different concentrations is added into biogas residues subjected to anaerobic fermentation, and then culture is performed through a culture medium, so that the conclusion that the yield of methane is obviously and regularly changed and the diversity and richness of microbial communities are respectively improved along with the increase of the concentration of sodium propionate is obtained; meanwhile, the optimal sodium propionate concentration, the optimal fermentation time and the like are determined when the methane daily yield and the methane cumulative yield are the highest, and the relative abundance of the firmicobacteria bacteria, the proteobacteria bacteria, the methanosarcina archaea and the methanomycorrhiza archaea is the maximum; therefore, the purposes of accurately controlling and remarkably increasing the methane yield and improving the microbial community composition are achieved.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for improving gas production by adding sodium propionate to domesticate microbial communities of an anaerobic fermentation system and its application. Background technique [0002] In recent years, with the improvement of national policies and people's quality, people's awareness of environmental protection is getting higher and higher. For various wastes generated in life, which cause great harm to the environment, anaerobic digestion technology has been proved to be effective in obtaining Biomass energy, an important method for treating agricultural waste, has the advantages of low energy consumption and high production capacity. At present, it has been widely industrialized and demonstrated in my country, and more and more biogas plants have been established to process various types of biogas. Agricultural waste, but for the biogas slurry an...

Claims

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Application Information

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IPC IPC(8): C12P5/02C12N1/20C12N1/36C12R1/01
CPCC12P5/023C12N1/20C12N1/36Y02W30/40
Inventor 王雅雅孙浩陈高攀李源源贾亚津
Owner HEBEI AGRICULTURAL UNIV.
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