57 results about "Anaeroarcus" patented technology

The invention relates to an extra-cellular polysaccharide of aerobic Ruthia sp. strain metabolin and preparation and application thereof. An aerobic strain ZHT4-13 is separated from adhesive sludge of wild Ruditapes philippinarums in the Bohai sea offshore of China and is identified as a Ruthia sp. The aerobic strain is treated by seed culture and amplification culture, and a fermentation broth of the aerobic strain is precipitated with ethanol and separated centrifugally to obtain the extra-cellular polysaccharide MBF4-13. The extra-cellular polysaccharide MBF4-13 is measured to have high flocculation activity, the FR value to kaolin reaches over 80 percent, and the removal rate to hexavalent chromium ions reaches 69.3 percent; the extra-cellular polysaccharide MBF4-13 has high activity to decolorize high-concentration wastewater, and the decolorization ratios to methylene blue, ink blue, malachite green and crystal violet reach 86.11 percent, 99.49 percent and 97.84 percent; and the extra-cellular polysaccharide MBF4-13 plays a role of obviously improving the performance and the structure of activated sludge. The extra-cellular polysaccharide has potential value of developing a novel microbial flocculant.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

The invention relates to the field of microorganisms, and particularly discloses a preparation method and application of a low-temperature efficient corn straw degrading bacterial agent. The bacterial agent is prepared from two single-plant bacteria, wherein the two single-plant bacteria are Pseudomonas sp. and Acinetobacter sp. and the preservation numbers are CGMCC No.20521 and CGMCC No.20522 respectively. According to the preparation method, the specific-composition bacteria genus is used as a bacteria source sample, a culture medium adapted to the bacteria source sample is used to perform fermentation propagation, the bacterial liquid fermented under the optimal fermentation condition cooperates with a suitable carrier and a protective agent, and a freeze drying technology is adopted to prepare the powdery bacterial agent. The bacterial agent can degrade corn straw under the low temperature condition, the preparation method is simple, the cost is low, the production period is short, and the problems that agricultural production is inconvenient due to the fact that straw cannot be degraded under the low-temperature condition after straw returning to the field in the north can be solved.

A person's intestinal (gut), oral or skin microbiota is modified using specific combinations of pre-biotics, pro-biotics and / or anti-biotics to establish a defined microbiota that can treat and / or reduce the likelihood that individuals will experience various diseases, including cancer. The employment of various bacteria, whether in particular combinations or after being modified using CRISPR-type systems, leads to improved outcomes when checkpoint inhibitors are used to treat various forms of cancer. One embodiment is directed to a method for reducing the likelihood of developing cancer by providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species. The level of Roseburia and Faecalibacterium prausnitzii, and / or Akkermansia muciniphila bacteria are increased in the individual's gut microbiome such that when an individual is administered an immune checkpoint inhibitor, its function is enhanced due to the presence of the bacterial population.

The invention discloses a TaqMan probe quantitative detection method for detecting pseudomonas putida and a corresponding kit. Specific gene detection is ingeniously applied to distinguish pseudomonasputida from strains or insect species of other species, and accurate bacterial genus information is obtained through comprehensive judgment. Compared with the existing mainstream detection kit, the kit provided by the invention for detecting the pseudomonas putida has the advantages of high sensitivity, rapidness, convenience, high specificity, rigorous and accurate judgment and the like, and hashigh application prospect and market value.

A polycyclic aromatic hydrocarbon degrading bacterium and its screening method and application relate to marine petroleum hydrocarbon degrading bacteria. In order to transform the heterologous alcanivorax (Alcanivorax xenomutans) 45II-AX, transform the heterologous alcanivorax into a Gram-negative strain of the genus Alcanivorax, with biological characteristics of non-fermentative, obligate aerobic, The shape of the bacteria is non-bacillus, the colony is round, yellow and opaque, the surface is smooth and moist, the edges are regular, no halo, the center is raised, the diameter is 2-3mm, the optimum growth conditions are: pH=7.0-8.5, temperature 25 ~28°C; can be used in the degradation of organic compounds. It was identified that the 16S rDNA gene sequence of a transformed heterologous alcanivorax was 99.93% similar to the type strain Alcanivorax xenomutans JC109(T).

The invention discloses a method for treating sewage by enrichment culture of anaerobic ammonium oxidation bacteria, and relates to a method for treating sewage, an integrated semi-nitrification anaerobic ammonium oxidation reactor is used in the method, and a water inlet tank is connected with a water inlet at the bottom of a reactor main body through a peristaltic pump; a filler frame is arranged in the reactor main body, an aeration head is arranged at the bottom in the reactor, the aeration amount of the aeration head is controlled by an aeration pump, and a pH online tester and a heatingrod are arranged at the middle upper part; the reactor main body is connected with an alkali barrel, and alkali is provided for the interior of the reactor through a liquid supplementing pump; and thereactor main body is connected with a sedimentation tank through a water outlet. When the system is used for sewage treatment, the device needs to be started firstly, then the ammonia nitrogen waterinlet load is increased, and humic acid is added in the experimental stage. According to the method, a proper amount of humic acid is added into the integrated semi-nitrification anaerobic ammonia oxidation reactor, and the proportion of anaerobic ammonia oxidation bacteria is increased, so that anaerobic ammonia oxidation bacteria are enriched, the denitrification capacity is improved, and the ammonia nitrogen removal rate can reach 95%.

A person's intestinal (gut), oral or skin microbiota is modified using specific combinations of pre-biotics, pro-biotics and / or anti-biotics to establish a defined microbiota that can treat and / or reduce the likelihood that individuals will experience various diseases, including cancer. The employment of various bacteria, whether in particular combinations or after being modified using CRISPR-type systems, leads to improved outcomes when checkpoint inhibitors are used to treat various forms of cancer. One embodiment is directed to a method for reducing the likelihood of developing cancer by providing in the gut of an individual a population of beneficial bacteria selected from the group consisting of Lactobacillus species. The level of Roseburia and Faecalibacterium prausnitzii, and / or Akkermansia muciniphila bacteria are increased in the individual's gut microbiome such that when an individual is administered an immune checkpoint inhibitor, its function is enhanced due to the presence of the bacterial population.

The invention belongs to the field of soil environment bioremediation, and further develops a bacteria-enzyme composite preparation for biodegradation of petroleum hydrocarbon pollutants in petroleumhydrocarbon contaminated soil, so as to realize efficient and rapid petroleum hydrocarbon contaminated soil bioremediation. The composite preparation is a microbial inoculum with petroleum hydrocarbondegradation capability and an enzyme agent obtained by crushing the microbial inoculum; the microbial inoculum is a culture solution which is obtained by carrying out fermentation culture on Variovorax sp. JWLC1 at the temperature of 30 + / -1 DEG C until the OD value is 1.5-2.5, wherein the volume ratio of the fermentation culture solution in the microbial inoculum to the fermentation culture solution in the enzyme agent is 1: 1 to 1: 3. The bacteria-enzyme composite preparation disclosed by the invention remarkably prolongs the microbial efficient degradation process of the petroleum hydrocarbon and improves the bioremediation efficiency of the petroleum hydrocarbon contaminated soil.

The invention relates to a mixed bacteria producing biosurfactant and a screening method thereof. The mixed bacteria is composed of three strains, named Pseudomonas stutzeri (Pseudomonas stutzeri) and the preservation numbers are CCTCC AB 205091, Composition of strains named Nocardioides ginsengagri with preservation number CCTCC S2013441 and Bacillus licheniformis with preservation number CCTCC AB 205141. 1. Activate and cultivate the selected oil production strains to be screened, conduct preliminary screening on the activated and cultured oil production strains to be screened by the blood plate method to obtain the primary screening fermentation liquid, and use the oil discharge ring method to re-screen the primary screening fermentation liquid after filtering. The mixed bacteria combines the advantages of the three strains and can produce biosurfactants used in oil recovery. The screening method of the mixed bacteria is widely applicable and effectively reduces the screening cost. In addition, the screening method has high accuracy and selectivity. Strong, convenient and easy-to-operate process, and compared with the prior art, the cycle is shorter.

The present invention describes novel bacterial L-amino acids α-ligases, which catalyzing reaction of dipeptide formation having an acidic L-amino acid such as L-Asp or L-Glu at the N-terminus. The method for producing dipeptides using said L-amino acids α-ligases and a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain the DNA encoding said L-amino acids α-ligases, is described.

The invention relates to the field of pesticide compounding techniques, particularly relates to a penconazole and zhongshengmycin-containing sterilization composition, and also relates to an application thereof. The penconazole and zhongshengmycin-containing sterilization composition comprises effective ingredients of penconazole and zhongshengmycin with a weight part ratio of (90:1)-(1:90), and the penconazole and zhongshengmycin-containing sterilization composition is prepared into a wettable powder, a suspending agent, an emulsion in water, a water dispersible granule, emulsifiable concentrate and the like after being added with assistants and an excipient, and is used for controlling fungal diseases caused by germs such as erysiphe, uromyces, mortierella, septoria and venturia and bacterial diseases caused by germs such as Gram-positive bacteria, Gram-negative bacteria, mycobacteria and saccharomycetes. The penconazole and zhongshengmycin-containing sterilization composition has a relatively good synergism within a certain range, is superior to a single preparation on the aspect of control effects, is relatively durable in pesticide effect, has a special effect on controlling powdery mildew, rust diseases, scab, leaf spot diseases, gray mold, white rot, soft rot, streak diseases, rice blast, ring spot, gibberellic diseases and bacterial diseases, is relatively low in residue, is relatively easily decomposed after a lasting period, is relatively environment-friendly, and is beneficial to the increase of yield and income in an agricultural district.

The invention discloses a myxobacterium H56D21 that preys on plant pathogenic bacteria and its application, belonging to the field of microbial technology and biological control. Analyze the ANI value and the dDDH value of bacterial strain H56D21 and similar type bacterial strains, the result shows that the H56D21 bacterial strain of the present invention is a Hypocystis ( Hyalangium ) of new species. of the present invention Hyalangium sp.H56D21, was deposited in Guangdong Microbial Culture Collection Center on November 17, 2020. The address is 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Yuexiu District, Guangzhou City, Guangdong Province, postcode: 510070, and the deposit number is: GDMCC No: 61294, which can prey on plant pathogenic bacteria and has broad-spectrum predation characteristics, and can be used for biological control of plant diseases.

The invention discloses a sea wormwood polysaccharide with the functions of anti-oxidation and regulating intestinal flora, as well as a preparation method and application thereof. The preparation method includes hot water extraction, deproteinization, decolorization, alcohol precipitation, dialysis and degradation; wherein, the degradation process is to add the crude polysaccharide solution of Artemisia annua with a concentration of 5-8 mg / mL, and add H 2 o 2 The solution reaches a volume concentration of 1-5%, is placed in an ultrasonic field for radiation treatment, the ultrasonic frequency is 20-25kHz, and the power is 450-550W, and freeze-dried to obtain sea wormwood polysaccharide. Compared with the crude polysaccharide of sea wormwood, the average molecular weight of the sea wormwood polysaccharide of the present invention is 130-510kDa, the particle size is 400-500nm, and the viscosity is low, which can significantly increase the abundance of beneficial bacteria in the human intestinal tract, while reducing harmful bacteria The abundance of intestinal microorganisms increases by about 7%, and it can be used as a food additive in functional foods that regulate intestinal health.

The present invention relates Pseudomonas strains and consortia thereof that are useful in protecting plants against microbial plant diseases caused by pathogens such as Ralstonia, Clavibacter, Erwinia, Curtobacterium, Fusarium, Phytophthora and Helminthosporium. The Pseudomonas strains were selected on the basis of their antagonistic abilities against plant pathogens such as production of antimicrobial compounds, direct inhibition of growth of plant pathogens, competition of carbon or nitrogen sources and endophytic features such as anaerobic growth on nitrate as electron acceptor and growth on arabinose as carbon source. The invention further relates to compositions comprising the strains or consortia of the invention, preferably lyophilized compositions, and to methods wherein they are used in protecting a wide variety of plants against a wide variety of microbial plant diseases.

Provided herein is a non plant-pathogenic strain that can stably exhibit a bacterial plant disease control effect at the actual site of crop production, and that can be safely used as a biopesticide against plant. A bacterial plant disease control agent using such a strain is also provided, among others. In the present invention, viable bacteria of a strain belonging to genus Mitsuaria, which wasnot known to have a control effect against bacterial plant disease, or a culture containing the viable bacteria of such a strain are used as an active component, and the invention provides bacterial plant disease control agents such as a vegetable wilt disease control agent, and a canker control agent.

The invention belongs to the field of soil environment remediation, and more specifically relates to a co-processing technology of electrodynamic remediation of organic pollutants and microbial remediation, and specifically relates to a functional bacterial strain suitable for remediation of oil-contaminated soil in the anode area of ​​a DC electric field and its application. The strain is Variovorax sp. JWLB3 (Variovorax sp. JWLB3), which was deposited in the China Center for Type Culture Collection on October 11, 2019, and its accession number is CCTCC NO: M 2019811. The phage genus JWLB3 is used in conjunction with electrokinetic restoration technology to remediate oil-contaminated soil in areas where a direct current electric field is applied, especially in contaminated soil in the anode area of ​​the electric field. When maintaining relatively appropriate water content conditions, the functional bacteria maintain high biological activity under the conditions of high acidity, high soluble salt ions, high chlorine content, high redox potential and high current intensity in the anode area of ​​electrokinetic restoration , and maintain a good ability to degrade petroleum hydrocarbons.