A functional bacterium suitable for remediation of oil-contaminated soil in the anode area of a direct current electric field and its application

A direct current electric field, polluted soil technology, applied in the field of soil environmental remediation, can solve the problems of weakening the overall remediation efficiency of electrokinetic enhanced microbial remediation technology, adverse effects on microbial abundance and activity, and accumulation of soluble inorganic ions, etc. The effect of adaptability, improving growth activity and improving application efficiency

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A direct current electric field, polluted soil technology, applied in the field of soil environmental remediation, can solve the problems of weakening the overall remediation efficiency of electrokinetic enhanced microbial remediation technology, adverse effects on microbial abundance and activity, and accumulation of soluble inorganic ions, etc. The effect of adaptability, improving growth activity and improving application efficiency

CN111440741BActive Publication Date: 2022-05-31SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI

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Experimental program

Comparison scheme

Effect test

Embodiment 1

[0033] Example 1 Voralea JWLB3 ( Variovorax sp. JWLB3) isolation, screening and identification

[0034] The bacterial source samples of functional strains come from the long-term oil-contaminated soil around oil wells in an oilfield area. After the soil samples are collected, they are subjected to direct current electric field domestication. The domestication process is completed in a box with a length of 26 cm, a width of 12 cm and a height of 10 cm. , direct current with a voltage difference of 24 V was passed into the soil of the box, and during the domestication process, water was regularly replenished in the anode area of the DC electric field, and the initial concentration of petroleum hydrocarbons in the soil reached 68 g·kg -1 . The acclimation period was 45 days, and the microorganisms in the oil-contaminated soil acclimated by the DC electric field were screened and cultured in the inorganic salt screening medium for 10 days. The total oil content in the medium w...

Embodiment 2

[0039] Example 2 Voralea JWLB3 ( Variovorax sp. JWLB3) Remediation of Petroleum Hydrocarbon Contaminated Soil under DC Electric Field

[0040] The greedy phage JWLB3 ( Variovorax sp. JWLB3) was inoculated into beef jerky peptone medium, the pH of the medium was adjusted to 3.5, incubated at 30°C and 180 rpm for 3 days with constant temperature shaking, and centrifuged at 8,000 rpm to collect the bacteria, and phosphate buffer was used to target the bacteria. Wash, use the aforementioned inorganic salt medium to resuspend the bacteria, and prepare the greedy phage JWLB3 ( Variovorax sp. JWLB3) bacterial suspension. The oil-contaminated soil to be treated is subjected to high temperature and high pressure steam sterilization to destroy indigenous microorganisms in the soil. The greedy phage JWLB3 ( Variovorax sp. JWLB3) was inoculated into the sterilized contaminated soil with an oil content of 4.6%, and the bacterial microbial abundance in the treated soil after inocula...

Embodiment 3

[0043] Example 3 Voralea JWLB3 ( Variovorax Remediation of petroleum hydrocarbon-contaminated soil by combined application of sp. JWLB3) and other functional inoculants under the condition of DC electric field

[0044] According to the description of the culture method in Example 2 and the prior art, respectively targeting the greedy phage JWLB3 ( Variovorax sp. JWLB3), Pseudomonas fluorescens ( Pseudomonas fluorescens ), Bacillus cereus ( Bacillus cereus ) and Bacillus licheniformis ( Bacillus licheniformis ) for cultivation and ready to use.

[0045] The greedy phage JWLB3 ( Variovorax sp. JWLB3) bacterial solution was mixed into the soil of the anode area, which accounted for 1 / 3 of the total length of the experimental device, and Pseudomonas fluorescens ( Pseudomonas fluorescens ), Bacillus cereus ( Bacillus cereus ) and Bacillus licheniformis ( Bacillus licheniformis) were simultaneously mixed into the soil in the middle zone and the cathode zone, which...

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Abstract

The invention belongs to the field of soil environment remediation, and more specifically relates to a co-processing technology of electrodynamic remediation of organic pollutants and microbial remediation, and specifically relates to a functional bacterial strain suitable for remediation of oil-contaminated soil in the anode area of a DC electric field and its application. The strain is Variovorax sp. JWLB3 (Variovorax sp. JWLB3), which was deposited in the China Center for Type Culture Collection on October 11, 2019, and its accession number is CCTCC NO: M 2019811. The phage genus JWLB3 is used in conjunction with electrokinetic restoration technology to remediate oil-contaminated soil in areas where a direct current electric field is applied, especially in contaminated soil in the anode area of the electric field. When maintaining relatively appropriate water content conditions, the functional bacteria maintain high biological activity under the conditions of high acidity, high soluble salt ions, high chlorine content, high redox potential and high current intensity in the anode area of electrokinetic restoration , and maintain a good ability to degrade petroleum hydrocarbons.

Description

technical field [0001] The invention belongs to the field of soil environmental remediation, and further relates to a synergistic treatment technology for electrodynamic remediation of organic pollutants and microbial remediation, in particular to a functional strain suitable for remediation of oil-contaminated soil in the anode area of a direct current electric field and its application. Background technique [0002] With the continuous development of the petroleum industry, the exploitation and demand of petroleum fossil fuels are increasing day by day, and the consequent oil pollution problem has become increasingly prominent. Oil-contaminated soil has become a hot and difficult issue in the environmental field. Various technical forms have been developed for the remediation of oil-contaminated soil, but each has its own advantages and disadvantages. Taking into account the restoration cost, economic benefits, ecological safety and restoration effect, etc., microbial re...

Claims

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Application Information

Patent Timeline

31 May 2022

Publication

CN111440741B

IPC: C12N1/20; B09C1/08; B09C1/10; C12R1/01; C12R1/39; C12R1/085; C12R1/10; C12R1/385; C12R1/32

CPC: C12N1/20; B09C1/085; B09C1/10; C12R2001/01; C12N1/205

Inventors: 郭书海; 王卅