System For Producing Terpenoids In Plants

Abstract

The invention concerns a method for producing terpenes of interest in plants having glandular trichomes, as well as plants useful for producing said terpenes of interest. Said plants comprise a sequence encoding a heterologous terpene synthase under the control of a promoter enabling it to be specifically expressed in the trichomes. Moreover, the pathway for producing endogenous diterpenes is preferably blocked in the trichomes of the plants, to increase the flow in the heterologous pathway. The secretion of heterologous terpenes is spontaneous resulting in easy collection. The present invention also concerns plants exhibiting a blocked production of a compound having antibiotic properties at the surface of leaves exhibiting enhanced efficiency of transformation by a bacterium.

Description

FIELD OF THE INVENTION[0001]The invention relates to a method for producing compounds of interest in plants and to genetically modified plants prepared for use in said method. The invention also relates to genetically modified plants enabling an enhanced efficiency of transformation.INTRODUCTION[0002]Trichomes are organs located at the surfaces of the aerial parts of higher plants (reviewed in Wagner et al., 2004). They take various forms and are classified into two main categories. The first includes hairs, or non-secreting trichomes, unicellular or multicellular. They do not secrete substances to the exterior, or at least not in appreciable amounts. The second group comprises all trichomes described as secreting or glandular, which have an increased capacity to synthesize and secrete various substances to the exterior. Several types of secreting trichomes are found in this category. In particular these include the peltate trichomes, from the Lamiaceae family for example (mint, bas...

AI Technical Summary

Benefits of technology

[0015]The present invention describes a novel method for producing terpenes of interest in plants having glandular trichomes, as well as plants useful for said production. Said novel method is based on introducing into the plant a heterologous terpene synthase enabling production of the terpene of interest. The expression of said heterologous terpene synthase is controlled by a promoter enabling an expression, preferably specific, in the glandular trichomes of the plant. To increase the yield, it is preferable to block the synthesis of endogenous diterpenes in the trichomes of the plant.

Problems solved by technology

These molecules are costly to produce due to the relatively low abundance of 10-DAB III and especially of paclitaxel in yew extracts, and due to the absence of a synthetic method that can be scaled up industrially, on account of the structural complexity of the molecules.

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