System For Producing Terpenoids In Plants

a technology of terpenoids and plants, applied in the field of systems for producing terpenoids in plants, can solve the problems of expensive production of molecules, and achieve the effect of increasing yield

Inactive Publication Date: 2008-11-13
PHILIP MORRIS PROD SA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0015]The present invention describes a novel method for producing terpenes of interest in plants having glandular trichomes, as well as plants useful for said production. Said novel method is based on introducing into the plant a heterologous terpene synthase enabling production of the terpen...

Problems solved by technology

These molecules are costly to produce due to the relatively low abundance of 10-DAB III and especially of paclitaxel in yew extracts,...

Method used

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  • System For Producing Terpenoids In Plants
  • System For Producing Terpenoids In Plants
  • System For Producing Terpenoids In Plants

Examples

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examples

Production of Taxadiene in the Tobacco Plant Nicotiana sylvestris by Expression of Taxadiene Synthase Under the Control of a Non-Specific Promoter

[0121]The constructs described below enabled an expression in all the cells of the plant, including in the trichomes. Said expression is described as non-specific of trichomes.

Expression Vector

[0122]The expression cassette was constructed as follows (see diagram in FIG. 1):

[0123]A constitutive promoter of the type 35S (extracted from cauliflower mosaic virus) well known to those skilled in the art.

[0124]Taxadiene synthase cDNA.

[0125]The OCS terminator (extracted from octopine synthase gene of an Agrobacterium tumefaciens Ti plasmid) well known to those skilled in the art.

[0126]The sequence of this construct is given in sequence SEQ ID No 1.

Analysis of Transformed Plants

[0127]The regeneration process led to the isolation of transgenic plants expressing taxadiene synthase. Plants containing a single copy of the transgene were selected and an...

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Abstract

The invention concerns a method for producing terpenes of interest in plants having glandular trichomes, as well as plants useful for producing said terpenes of interest. Said plants comprise a sequence encoding a heterologous terpene synthase under the control of a promoter enabling it to be specifically expressed in the trichomes. Moreover, the pathway for producing endogenous diterpenes is preferably blocked in the trichomes of the plants, to increase the flow in the heterologous pathway. The secretion of heterologous terpenes is spontaneous resulting in easy collection. The present invention also concerns plants exhibiting a blocked production of a compound having antibiotic properties at the surface of leaves exhibiting enhanced efficiency of transformation by a bacterium.

Description

FIELD OF THE INVENTION[0001]The invention relates to a method for producing compounds of interest in plants and to genetically modified plants prepared for use in said method. The invention also relates to genetically modified plants enabling an enhanced efficiency of transformation.INTRODUCTION[0002]Trichomes are organs located at the surfaces of the aerial parts of higher plants (reviewed in Wagner et al., 2004). They take various forms and are classified into two main categories. The first includes hairs, or non-secreting trichomes, unicellular or multicellular. They do not secrete substances to the exterior, or at least not in appreciable amounts. The second group comprises all trichomes described as secreting or glandular, which have an increased capacity to synthesize and secrete various substances to the exterior. Several types of secreting trichomes are found in this category. In particular these include the peltate trichomes, from the Lamiaceae family for example (mint, bas...

Claims

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Application Information

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IPC IPC(8): C12N15/60A01H5/00C10G1/04C12N15/113
CPCC12N9/88C12N15/1137C12N15/8205C12P15/00C12N15/8243C12P5/007C12N15/8223
Inventor TISSIER, ALAINSALLAUD, CHRISTOPHERONTEIN, DENIS
Owner PHILIP MORRIS PROD SA
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