Suckling pig milk replacer capable of improving intestinal function and preparation method of suckling pig milk replacer

Abstract

The invention discloses a suckling pig creep feed capable of improving intestinal functions and a preparation method of the suckling pig creep feed, and belongs to the technical field of feeds. The creep feed comprises the following components in parts by weight: 590 to 750 parts of a basic component containing protein, 1.5 to 3.0 parts of coated tannic acid, 20 to 30 parts of a basic component containing lipid, 0.1 to 0.3 part of glucose oxidase, 0.2 to 0.5 part of a macleaya cordata extract, 0.05 to 0.1 part of baicalin, 0.1 to 0.3 part of chlorogenic acid, 0.5 to 1.5 parts of butyrin, 2.0 to 4.0 parts of benzoic acid and the like. Zinc oxide replacement is achieved through multi-target combination, the diarrhea rate of suckling pigs is reduced, and the intestinal function is improved; the problem of antagonism of tannic acid and feed protein is solved by a chitosan-liposome double-layer coating technology; by adding nano silicon dioxide, the problem of feed caking is solved, and the growth performance of suckling pigs is comprehensively improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of feeds, and relates to a creep feed for sucking pigs for improving intestinal function and a preparation method thereof. Background Art

[0002] Due to factors such as underdeveloped digestive systems and environmental stress, weaned piglets are prone to problems such as diarrhea, decreased growth performance, and increased mortality.

[0003] In order to deal with diarrhea, a relatively popular related technology is to add high-dose zinc oxide and antibiotics to control diarrhea in piglets. However, although creep feeds with high-dose zinc oxide and antibiotics can control diarrhea, long-term use of zinc oxide can cause environmental pollution (excessive zinc emissions), rough and disordered hair in piglets, intestinal flora imbalance, and the risk of drug resistance.

[0004] Some researchers have tried to use tannic acid to replace high-dose zinc oxide to solve the problem of diarrhea in piglets, but tannic acid has strong chemical activity and is extremely easy to react with protein components in the feed to form complexes that are difficult for piglets to digest and absorb, ultimately resulting in the waste of key nutrients in the feed and affecting the normal growth and development of piglets. Summary of the Invention

[0005] The purpose of the present invention is to provide a creep feed for sucking pigs for improving intestinal function and a preparation method thereof, effectively reducing the diarrhea rate and promoting nutrient absorption.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] In the first aspect, the present invention proposes a creep feed for sucking pigs for improving intestinal function, adopting the following technical solutions:

[0008] A creep feed for sucking pigs for improving intestinal function, comprising the following components in parts by weight:

[0009]

[0010]

[0011] Among them, the coated tannic acid is coated with a chitosan solution.

[0012] Further, the coated tannic acid has a double-layer coating structure. The inner layer of the coated tannic acid is chitosan, and the outer layer of the coated tannic acid is coated with a liposome suspension, and the liposome suspension is prepared by dissolving soybean phospholipid and cholesterol in a mass ratio of (2-3):1 in phosphate buffer solution.

[0013] Further, silica is added to the outer layer of the coated tannic acid.

[0014] Preferably, the creep feed further comprises 0.05 - 0.1 parts by weight of an antioxidant, and the antioxidant is selected from natural plant extracts. Preferably, the antioxidant is rosemary extract.

[0015] Preferably, the creep feed further comprises 0.5 - 1.0 parts by weight of a compound probiotic, and the compound probiotic comprises Lactobacillus plantarum, Enterococcus faecalis and Bacillus subtilis.

[0016] Preferably, the creep feed further comprises 0.9 - 1.5 parts by weight of glycine chelated trace elements, and the glycine chelated trace elements comprise zinc glycinate, iron glycinate and copper glycinate. Preferably, the weight of zinc glycinate is 0.5 - 0.8 parts by weight, the weight of iron glycinate is 0.3 - 0.5 parts by weight, and the weight of copper glycinate is 0.1 - 0.2 parts by weight.

[0017] Preferably, the creep feed further comprises 0.5 - 1.0 parts by weight of a compound vitamin, and the compound vitamin comprises vitamin A, vitamin D3, vitamin E and vitamin C.

[0018] Preferably, the basic component comprises the following components in parts by weight:

[0019]

[0020] Preferably, the lipid-containing basic component is fat powder.

[0021] In the second aspect, the present invention provides a preparation method of a creep feed for suckling pigs to improve intestinal function, and the following technical scheme is adopted:

[0022] A preparation method of a creep feed for suckling pigs to improve intestinal function, comprising the following steps:

[0023] Z1. Raw material pretreatment: fully pulverize the protein-containing basic component and the lipid-containing basic component to obtain a basic material;

[0024] Z2. Mixing: fully mix the basic material with tannic acid coated, Macleaya cordata extract, glucose oxidase, baicalin, chlorogenic acid, glycerol tributyrate and benzoic acid to obtain a mixture;

[0025] Z3. Pelleting: pelletize the mixture.

[0026] The beneficial effects of the present invention:

[0027] 1. The physical isolation of tannic acid is achieved by using the chitosan-liposome double-layer coating technology, effectively solving the antagonism problem between tannic acid and the protein components in the feed. The inner layer of chitosan coating forms a dense barrier, blocking the contact reaction between tannic acid and the protein in the feed and avoiding the nutrient loss caused by the formation of complexes. The outer layer of liposome coating further blocks the active groups of tannic acid through the phospholipid bilayer structure, maintaining the structural integrity in the acidic environment of the stomach. When reaching the neutral environment of the intestine, the liposome layer intelligently releases tannic acid through enzymatic hydrolysis, enabling it to act on the intestinal mucosa to exert the astringent and antibacterial functions, not only retaining the biological activity of tannic acid but also avoiding the ineffective binding with feed nutrients.

[0028] 2. The present invention achieves the replacement of zinc oxide in the starter feed by adding tannic acid and Macleaya cordata extract as antibacterial components, adding chlorogenic acid and baicalin as anti-inflammatory components, adding glycerol butyrate as a repair component, and adding compound probiotics for microecological regulation. Tannic acid astringes the intestinal mucosal protein and inhibits the adhesion of Escherichia coli. The Macleaya cordata extract contains alkaloids with broad-spectrum antibacterial properties. The two work synergistically to effectively combat harmful bacteria and reduce the risk of the intestine being invaded by pathogens. Benzoic acid reduces the intestinal pH value to inhibit harmful bacteria and creates a good living environment for beneficial bacteria. Glycerol butyrate slowly releases butyric acid to repair the intestinal mucosa and helps the damaged intestinal tissue recover its normal structure and function. Compound probiotics competitively exclude pathogenic bacteria and regulate immunity, strengthening the intestinal health defense from both the aspects of flora balance and body immunity. Chlorogenic acid inhibits the expression of inflammatory factors, and baicalin enhances the tight junction proteins to relieve stress damage. The synergistic cooperation of tannic acid, Macleaya cordata extract, chlorogenic acid, baicalin, and compound probiotics acts on the intestine in all aspects, significantly reducing the diarrhea rate of suckling pigs, improving the intestinal morphology and function, achieving the efficient and healthy management of the suckling pig intestine without adding zinc oxide, and promoting the improvement of the growth performance of suckling pigs.

[0029] 3. By adding nano-silica in the liposome coating layer, the caking problem in feed processing and storage is significantly improved. The silica particles form a physical barrier layer on the surface of the coated tannic acid. By reducing the contact area and hygroscopicity between particles, it effectively prevents the feed containing tannic acid components from sticking and caking during high-temperature granulation or in a high-humidity environment. At the same time, its nano-scale particle size can be evenly dispersed in the coating layer, ensuring the anti-caking effect without affecting the release performance of the active ingredients, and enabling the starter feed to maintain good fluidity and component uniformity. Detailed implementation mode

[0030] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the following, in combination with embodiments, details the specific implementation mode, structure, characteristics, and their effects according to the present invention as follows.

[0031] The Macleaya cordata extract involved in the present invention was purchased from Fufeng Sinuote Biotechnology Co., Ltd.;

[0032] The rosemary extract was purchased from Hebei Hongtao Bioengineering Co., Ltd.;

[0033] Chlortetracycline was purchased from Shandong Kangrong Pharmaceutical Co., Ltd.;

[0034] The Lactobacillus plantarum was purchased from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the strain number was CGMCC 1.12974;

[0035] The Enterococcus faecalis was purchased from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the strain number was CGMCC 1.15424;

[0036] The Bacillus subtilis was purchased from the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, and the strain number was CGMCC1.15792.

[0037] Example 1

[0038] A creep feed for suckling pigs to improve intestinal function, comprising the following components in parts by weight:

[0039]

[0040] Among them, the basic components include the following components in parts by weight:

[0041]

[0042] The lipid-containing basic component is fat powder.

[0043] The coated tannic acid is coated with a 1% chitosan solution.

[0044] Further, the coated tannic acid is a double-layer coating structure. The inner layer of the coated tannic acid is chitosan, and the outer layer of the coated tannic acid is coated with a liposome suspension. The liposome suspension is prepared by dissolving soybean phospholipid and cholesterol in a mass ratio of 3:1 in phosphate buffer solution.

[0045] Further, nano-silica is added to the outer layer of the coated tannic acid.

[0046] The preparation method of the coated tannic acid is specifically as follows:

[0047] S1. Weigh 1.5 g of tannic acid and add it to a 1% chitosan solution. Stir at a speed of 800 r / min for 30 min, and then transfer it to a spray dryer for spray drying to obtain a particulate mixture a with a core coating layer. Among them, the pH value of the chitosan solution is 4.5, and the process parameters of spray drying are: the feeding rate is 5 mL / min, the atomization pressure is 0.2 MPa, the inlet air temperature is 80 °C, and the outlet air temperature is 50 °C.

[0048] S2. Add the particulate mixture a to a liposome suspension containing nano-silica. Stir at a speed of 600 r / min at 35 °C for 2 h, and form an outer liposome coating by the thin film dispersion method. Finally, transfer the coated particles to a rotary evaporator and dry them under the conditions of 40 °C and a vacuum degree of -0.08 MPa to obtain double-coated particles with nano-silica added to the outer layer. Among them, the preparation of the liposome suspension containing nano-silica is as follows: Weigh 3 g of soybean phospholipid, 1 g of cholesterol, and 0.3 g of nano-silica (particle size 20 - 50 nm) according to a mass ratio of 3:1, dissolve them in 100 mL of phosphate buffer solution (PBS) with a pH of 7.4, and then ultrasonically treat them for 30 minutes under the conditions of 300 W and 40 kHz to form a liposome suspension containing nano-silica.

[0049] The antioxidant is rosemary extract. The glycine-chelated trace elements include zinc glycinate, iron glycinate, and copper glycinate. The weight portion of zinc glycinate is 0.5 portion, the weight portion of iron glycinate is 0.3 portion, and the weight portion of copper glycinate is 0.1 portion. The preparation method of the glycine-chelated trace elements includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir them evenly to obtain the glycine-chelated trace elements.

[0050] The compound probiotics include Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis. Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are mixed according to a weight ratio of 5:3:2, and the viable counts of Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are all ≥ 1×10^8 CFU / g;

[0051] The compound vitamins contain the following components and weight portions: vitamin A acetate powder (500000 IU / g) 0.02 portion, vitamin D3 powder (500000 IU / g) 0.01 portion, vitamin E powder (50%) 0.2 portion, vitamin C 1 portion, carrier (corn starch) 98.77 portions. The preparation method of the compound vitamins includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir them evenly to obtain the compound vitamins.

[0052] A preparation method of a creep feed for suckling pigs to improve intestinal function, comprising the following steps:

[0053] Z1. Raw material pretreatment: Use a universal grinder to fully grind the protein-based components (expanded corn, expanded soybean, fish meal, whey powder) and lipid-based components (fat powder) to a particle size of ≤ 0.5 mm. Transfer the ground raw materials to a V-type mixer and mix at a speed of 200 r / min for 30 min to obtain a basic feed;

[0054] Z2. Mixing: Add the basic feed, Macleaya cordata extract, compound probiotics, glycine chelated trace elements, compound vitamins, antioxidants, coated tannic acid, chlorogenic acid, baicalin, glycerol butyrate, benzoic acid, and glucose oxidase to a V-type mixer and mix at a speed of 250 r / min for 10 minutes to obtain a mixture;

[0055] Z3. Pelleting: Place the mixture in a ring die pellet mill, control the pelleting temperature at 55 °C and the pressure at 5 MPa to make creep feed pellets with a particle diameter of about 3 mm. Transfer the pelleted creep feed pellets to an electrothermal blast drying oven and dry at 60 °C until the moisture content is ≤ 10%.

[0056] Example 2

[0057] A creep feed for suckling pigs to improve intestinal function, comprising the following components in parts by weight:

[0058]

[0059] Among them, the basic components include the following components in parts by weight:

[0060]

[0061] The lipid-based basic component is fat powder.

[0062] The coated tannic acid is coated with a 2% chitosan solution.

[0063] Furthermore, the coated tannic acid has a double-layer coating structure. The inner layer of the coated tannic acid is chitosan, and the outer layer of the coated tannic acid is coated with a liposome suspension. The liposome suspension is prepared by dissolving soybean phospholipid and cholesterol in a mass ratio of 2:1 in phosphate buffer solution.

[0064] Furthermore, nano-silica is added to the outer layer of the coated tannic acid.

[0065] The preparation method of the coated tannic acid is specifically as follows:

[0066] S1. Weigh 2.25 g of tannic acid and add it to a 2% chitosan solution. Stir at a speed of 800 r / min for 30 min, and then transfer it to a spray dryer for spray drying to obtain a particulate mixture a with an inner core coating layer. Among them, the pH value of the chitosan solution is 4.5, and the process parameters of spray drying are as follows: the feeding rate is 5 mL / min, the atomizing pressure is 0.2 MPa, the inlet air temperature is 80 °C, and the outlet air temperature is 50 °C.

[0067] S2. Add the particulate mixture a to a liposome suspension containing nano-silica. Stir at a speed of 600 r / min at 35 °C for 2 h, and form an outer liposome coating by the thin film dispersion method. Finally, transfer the coated particles to a rotary evaporator and dry them under the conditions of 40 °C and a vacuum degree of -0.08 MPa to obtain double-coated particles with nano-silica added to the outer layer. Among them, the preparation of the liposome suspension containing nano-silica is as follows: Weigh 2 g of soybean phospholipid, 1 g of cholesterol, and 0.2 g of nano-silica (particle size 20 - 50 nm) according to a mass ratio of 2:1, dissolve them in 100 mL of phosphate buffer solution (PBS) with a pH of 7.4, and then ultrasonically treat them for 30 minutes under the conditions of 300 W and 40 kHz to form a liposome suspension containing nano-silica.

[0068] The antioxidant is rosemary extract. The glycine chelated trace elements include zinc glycinate, iron glycinate, and copper glycinate. The weight portion of zinc glycinate is 0.65 portion, the weight portion of iron glycinate is 0.4 portion, and the weight portion of copper glycinate is 0.15 portion. The preparation method of the glycine chelated trace elements includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir them evenly to obtain the glycine chelated trace elements.

[0069] The compound probiotics include Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis. Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are mixed according to a weight ratio of 5:3:2, and the viable counts of Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are all ≥ 1×10^8 CFU / g;

[0070] The compound vitamins contain the following components and weight portions: vitamin A acetate powder (500000 IU / g) 0.02 portion, vitamin D3 powder (500000 IU / g) 0.01 portion, vitamin E powder (50%) 0.2 portion, vitamin C 1 portion, carrier (corn starch) 98.77 portions. The preparation method of the compound vitamins includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir them evenly to obtain the compound vitamins.

[0071] A preparation method of creep feed for suckling pigs to improve intestinal function, comprising the following steps:

[0072] Z1. Raw material pretreatment: Use a universal pulverizer to fully pulverize the protein-based components (expanded corn, expanded soybean, fish meal powder, whey powder) and lipid-based components (fat powder) to a particle size of ≤ 0.5 mm. Transfer the pulverized raw materials to a V-type mixer and mix at a speed of 200 r / min for 30 min to obtain the basic feed.

[0073] Z2. Mixing: Add the basic feed, Macleaya cordata extract, compound probiotics, glycine chelated trace elements, compound vitamins, antioxidants, coated tannic acid, chlorogenic acid, baicalin, glycerol butyrate, benzoic acid, and glucose oxidase to a V-type mixer and mix at a speed of 250 r / min for 10 minutes to obtain a mixture.

[0074] Z3. Pelleting: Put the mixture into a ring die pellet mill, control the pelleting temperature at 55 °C and the pressure at 5 MPa to make creep feed pellets with a particle diameter of about 3 mm. Transfer the pelleted creep feed pellets to an electrothermal blast drying oven and dry at 60 °C until the moisture content ≤ 10%.

[0075] Example 3

[0076] A creep feed for suckling pigs to improve intestinal function, comprising the following components in parts by weight:

[0077]

[0078]

[0079] Among them, the basic components include the following components in parts by weight:

[0080]

[0081] The lipid-based basic component is fat powder.

[0082] The coated tannic acid is coated with a 3% chitosan solution.

[0083] Furthermore, the coated tannic acid has a double-layer coating structure. The inner layer of the coated tannic acid is chitosan, and the outer layer of the coated tannic acid is coated with a liposome suspension. The liposome suspension is prepared by dissolving soybean phospholipid and cholesterol in a mass ratio of 3:1 in phosphate buffer solution.

[0084] Furthermore, nano-silica is added to the outer layer of the coated tannic acid.

[0085] The preparation method of the coated tannic acid is specifically as follows:

[0086] S1. Weigh 3.0 g of tannic acid and add it to a 3% chitosan solution. Stir at a speed of 800 r / min for 30 min, then transfer it to a spray dryer for spray drying to obtain a particulate mixture a with a core coating layer. Among them, the pH value of the chitosan solution is 4.5, and the process parameters of spray drying are: the feeding rate is 5 mL / min, the atomization pressure is 0.2 MPa, the inlet air temperature is 80 °C, and the outlet air temperature is 50 °C.

[0087] S2. Add the particulate mixture a to a liposome suspension containing nano-silica. Stir at a speed of 600 r / min at 35 °C for 2 h, and form an outer liposome coating by the thin-film dispersion method. Finally, transfer the coated particles to a rotary evaporator and dry them under the conditions of 40 °C and a vacuum degree of -0.08 MPa to obtain double-coated particles with nano-silica added to the outer layer. Among them, the preparation of the liposome suspension containing nano-silica is as follows: Weigh 3 g of soybean phospholipid, 1 g of cholesterol, and 0.3 g of nano-silica (particle size 20 - 50 nm) according to a mass ratio of 3:1, dissolve them in 100 mL of phosphate buffer solution (PBS) with a pH of 7.4, and then ultrasonically treat them for 30 minutes under the conditions of 300 W and 40 kHz to form a liposome suspension containing nano-silica.

[0088] The antioxidant is rosemary extract. The glycine chelated trace elements include zinc glycinate, iron glycinate, and copper glycinate. The weight portion of zinc glycinate is 0.8 parts, the weight portion of iron glycinate is 0.5 parts, and the weight portion of copper glycinate is 0.2 parts. The preparation method of the glycine chelated trace elements includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir well to obtain the glycine chelated trace elements.

[0089] The compound probiotics include Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis. Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are mixed according to a weight ratio of 5:3:2, and the viable counts of Lactobacillus plantarum, Enterococcus faecalis, and Bacillus subtilis are all ≥ 1×10^8 CFU / g.

[0090] The compound vitamin contains the following components and weight portions: vitamin A acetate powder (500000 IU / g) 0.02 parts, vitamin D3 powder (500000 IU / g) 0.01 parts, vitamin E powder (50%) 0.2 parts, vitamin C 1 part, carrier (corn starch) 98.77 parts. The preparation method of the compound vitamin includes the following steps: Accurately weigh each component according to the above weight portions, put the weighed components into a clean container, and stir well to obtain the compound vitamin.

[0091] A preparation method of a creep feed for suckling pigs to improve intestinal function, comprising the following steps:

[0092] Z1. Raw material pretreatment: Use a universal grinder to fully grind the protein-based components (expanded corn, expanded soybean, fish meal, whey powder) and lipid-based components (fat powder) to a particle size ≤ 0.5 mm. Transfer the ground raw materials to a V-type mixer and mix at a speed of 200 r / min for 30 min to obtain the basic feed;

[0093] Z2. Mixing: Add the basic feed, Macleaya cordata extract, compound probiotics, glycine chelated trace elements, compound vitamins, antioxidants, coated tannic acid, chlorogenic acid, baicalin, glycerol tributyrate, benzoic acid, and glucose oxidase to a V-type mixer and mix at a speed of 250 r / min for 10 minutes to obtain a mixture;

[0094] Z3. Pelleting: Put the mixture into a ring die pellet mill, control the pelleting temperature at 55 °C and the pressure at 5 MPa to make creep feed pellets with a particle diameter of about 3 mm. Transfer the pelleted creep feed pellets to an electrothermal blast drying oven and dry at 60 °C until the moisture content ≤ 10%.

[0095] Control Example 1

[0096] The creep feed of this control example comprises the following components in parts by weight:

[0097]

[0098] Among them, the antibiotic is chlortetracycline. The glycine chelated trace elements include zinc glycinate, iron glycinate, and copper glycinate. The weight of zinc glycinate is 0.8 part, the weight of iron glycinate is 0.5 part, and the weight of copper glycinate is 0.2 part.

[0099] The compound vitamins contain the following components and weight fractions: vitamin A acetate powder (500000 IU / g) 0.02 part, vitamin D3 powder (500000 IU / g) 0.01 part, vitamin E powder (50%) 0.2 part, vitamin C 1 part, carrier (corn starch) 98.77 parts.

[0100] The preparation method of the compound vitamins comprises the following steps: Accurately weigh each component according to the above weight fractions, put the weighed components into a clean container, and stir well to obtain the compound vitamins.

[0101] The preparation method of the creep feed of this control example is as follows:

[0102] Z1. Pretreatment of raw materials: Use a universal crusher to crush expanded corn, plasma protein powder, expanded soybean, fish meal powder, whey powder and fat to a particle size of ≤0.5 mm, and then transfer the crushed raw materials to a V-type mixer and mix at a speed of 200 r / min for 30 min to obtain a basic material;

[0103] Z2. Mixing: Add the basic material, glycine chelated trace elements, compound vitamins, antibiotic (chlortetracycline) and zinc oxide to a V-type mixer and mix at a speed of 250 r / min for 10 minutes to obtain a mixture;

[0104] Z3. Pelleting: Transfer the mixture to a ring die pellet mill, control the pelleting temperature at 55 °C and the pressure at 5 MPa to make starter feed pellets with a particle diameter of 3 mm, and then transfer the pelleted starter feed pellets to an electrothermal blast drying oven and dry at 60 °C until the moisture content is ≤10%.

[0105] Comparative Example 1

[0106] The difference from Example 3 is that: the tannic acid is not coated and chitosan with the same dosage as the coated tannic acid is added at the same time.

[0107] Comparative Example 2

[0108] The difference from Example 3 is that: tannic acid, Macleaya cordata extract, chlorogenic acid, baicalin, compound probiotics and glycerol butyrate are omitted.

[0109] Comparative Example 3

[0110] The difference from Example 3 is that: nano-silica is not added to the outer layer of the tannic acid.

[0111] Experimental Example 1

[0112] In vitro simulated intestinal digestion experiment

[0113] In this experiment, an experimental group (Example 3) and a control group (Comparative Example 1) were set up, and each group had 3 parallel samples.

[0114] Pretreatment before the experiment:

[0115] Sample crushing: Crush the starter feeds of Example 3 and Comparative Example 1 respectively with a universal crusher, pass through a 60-mesh sieve (aperture 0.25 mm) to obtain a uniform powder, and then accurately weigh 1.0 g of the powder sample and place it in a 50 mL centrifuge tube.

[0116] Preparation of simulated intestinal fluid: Take 0.68 g of potassium dihydrogen phosphate and 7.16 g of disodium hydrogen phosphate dodecahydrate, add them to 800 mL of deionized water, stir magnetically until completely dissolved, adjust the pH to 7.0 ± 0.2, then make up the volume to 1 L, filter and sterilize through a 0.22 μm filter membrane, and store at 4 °C for later use; then take 100 mL of the above buffer solution, preheat it to 37 °C, add 0.5 g of pancreatin (activity ≥ 250 U / mg) and 0.3 g of porcine bile salt, stir magnetically (500 rpm) for 30 minutes until dissolved, and prepare it immediately before use to ensure enzyme activity.

[0117] Experimental procedure: Add 20 mL of simulated intestinal fluid to the centrifuge tube containing the sample, vortex for 30 seconds to fully wet the sample, then place the centrifuge tube in a thermostatic shaker, set at 37 °C and 120 rpm for continuous reaction for 4 hours. During the reaction, take 5 mL of the sample every 60 min, immediately terminate the reaction by ice bath for 5 minutes, and take the supernatant for measurement after centrifuging at 8000×g for 10 minutes.

[0118] Testing: Take the supernatant after terminating the reaction, use the Kjeldahl method, calculate the residual amount of undigested protein by measuring the total nitrogen content, and the lower the value, the more thorough the protein digestion.

[0119] The data of Experimental Example 1 are shown in Table 1.

[0120] Table 1

[0121]

[0122] Note: * indicates p < 0.05 compared with the data of the control group at the same time; ** indicates p < 0.01 (two-way ANOVA and Tukey test)

[0123] It can be seen from the data in Table 1 that during the 4-hour simulated intestinal digestion process, the protein concentration in the experimental group (Example 3) was always significantly lower than that in the control group (Comparative Example 1). At 60 minutes, the protein concentration in the experimental group decreased by 31% compared with the control group (p < 0.05); after 120 minutes, the difference further widened to 42% (p < 0.01); at 240 minutes, the gap reached 48% (p < 0.01).

[0124] The above results clearly show that the chitosan-liposome bilayer coating technology has played an important role. Uncoated tannic acid will rapidly release reactive groups in the neutral intestinal fluid environment, form hydrogen bonds and hydrophobic interactions with protein molecules, resulting in the solidification of the protein spatial structure, making it difficult for pancreatic enzymes to recognize and hydrolyze peptide bonds. In the experimental group, through the bilayer coating structure, the inner layer of chitosan forms a physical barrier in the acidic gastric environment, and the outer layer of liposomes is gradually degraded by pancreatic lipase in the intestinal fluid, thus cleverly realizing the slow release of tannic acid. This precise release mode ensures that tannic acid contacts the intestinal mucosa only after the protein is fully digested, not only retaining its astringent and antibacterial effects, but also effectively avoiding the antagonistic effect on nutrients, and finally significantly improving the protein utilization rate.

[0125] Experimental Example 2

[0126] 100 healthy 21-day-old weaned Duroc×Landrace×Yorkshire three-way cross piglets with an initial body weight of (6.5±0.3) kg were selected. The piglets were randomly divided into 5 groups according to body weight and gender, with 20 piglets in each group (half male and half female).

[0127] The grouping is as follows:

[0128] Example 3 group: Fed the creep feed for piglets for improving intestinal function described in Example 3.

[0129] Control Example 1 group: Fed the creep feed described in Control Example 1.

[0130] Comparative Example 1 group: Fed the creep feed described in Comparative Example 1.

[0131] Comparative Example 2 group: Fed the creep feed described in Comparative Example 2.

[0132] Comparative Example 3 group: Fed the creep feed described in Comparative Example 3.

[0133] The experiment lasted for 28 days, divided into an adaptation period (the first 3 days) and a formal experimental period (25 days).

[0134] Feed feeding:

[0135] During the adaptation period, all piglets were allowed to freely eat and drink to gradually adapt them to the new environment and feed. During the formal experimental period, the feed was fed regularly and quantitatively every day, 4 times a day, at 07:00, 11:00, 15:00 and 19:00 respectively. The feeding amount each time was adjusted according to the body weight and feeding situation of the piglets to ensure that each piglet could obtain enough feed.

[0136] Testing:

[0137] (1) Growth performance indicators:

[0138] Body weight: At the beginning and end of the experiment, each piglet was weighed on an empty stomach, the initial weight and final weight were recorded, and the average daily gain was calculated.

[0139] Average daily feed intake: By recording the feed intake and remaining amount of each group of piglets every day, the average daily feed intake was calculated.

[0140] Feed conversion ratio: The feed conversion ratio was calculated based on the average daily gain and average daily feed intake.

[0141] (2) Intestinal health indicators:

[0142] Diarrhea rate: Observe the feces of piglets every day, record the number of diarrheic piglets and the number of diarrhea episodes. The judgment standard for diarrhea is that the feces are in a thin paste or watery state. The diarrhea rate calculation formula is: Diarrhea rate (%) = (Number of diarrheic piglets × Number of diarrhea episodes) / (Total number of piglets × Number of experimental days) × 100%.

[0143] The test data in Experimental Example 2 are shown in Table 2.

[0144] Table 2

[0145]

[0146] Note: Different superscript letters for data in the same row indicate significant differences (P < 0.05), and the same letter or no letter indicates no significant difference.

[0147] As can be seen from Table 2:

[0148] 1. The average daily gain of the Example 3 group reached 298.6 ± 18.5 g / d, significantly higher than that of other groups (P < 0.05). The average daily feed intake was 485.2 ± 22.3 g / d, also significantly higher than that of Comparative Example 1 group, Comparative Example 2 group and Comparative Example 3 group (P < 0.05). The feed conversion ratio was as low as 1.63 ± 0.07, and the diarrhea rate was only 5.8 ± 0.7%. This fully shows that the creep feed of the present invention has significant effects in promoting the growth of suckling pigs, increasing feed intake, optimizing the feed conversion ratio and improving intestinal health through the synergistic effect of "antibacterial - anti - inflammatory - repair - regulation" multi - targets, and can provide sufficient nutrition for suckling pigs and effectively maintain the normal function of the intestine.

[0149] 2. The Comparative Example 1 group used a creep feed containing zinc oxide and antibiotics. Its average daily gain was 285.4 ± 20.1 g / d, the diarrhea rate was 6.5 ± 0.9%, and the feed conversion ratio was 1.68 ± 0.08. Compared with the Example 3 group, the average daily gain, feed conversion ratio and diarrhea rate were all at a disadvantage. This result proves that the zinc - oxide - free formula adopted in the present invention has successfully replaced the traditional formula containing zinc oxide, has obvious advantages in ensuring the growth performance and intestinal health of suckling pigs, and avoids problems such as environmental pollution caused by the use of high - dose zinc oxide.

[0150] 3. In the tannic acid group of Comparative Example 1, the tannic acid was not coated. Its average daily weight gain was 263±16 g / d, the diarrhea rate increased to 9.2±1.1%, and the feed conversion ratio was 1.72±0.09, all of which were significantly higher than those in the third example group (P<0.05). This clearly shows the importance of coating tannic acid with the chitosan-liposome double-layer coating technology. Uncoated tannic acid will react with the proteins in the feed, affecting the digestion and absorption of proteins, and further affecting the growth performance and intestinal health of piglets. Coated tannic acid can effectively avoid this problem and better exert its antibacterial and astringent effects.

[0151] 4. In the second comparative example group, tannic acid, Macleaya cordata extract, chlorogenic acid, baicalin, compound probiotics and glycerol butyrate were removed. Its average daily weight gain decreased to 231±15 g / d, the average daily feed intake was only 415±18 g / d, the feed conversion ratio increased to 1.80±0.10, and the diarrhea rate was as high as 14.6±1.5%, all of which were significantly worse than those in the third example group (P<0.05). This fully shows that these components such as tannic acid, Macleaya cordata extract, chlorogenic acid, baicalin, compound probiotics and glycerol butyrate play a crucial role in the creep feed synergistically. Through various functions such as antibacterial, anti-inflammatory, repair and regulation, they jointly maintain the normal function of the intestine and promote the growth of piglets.

[0152] 5. In the third comparative example group, nano-silica was not added to the outer layer of tannic acid. Its average daily weight gain was 276±17 g / d, the diarrhea rate was 7.3±0.8%, and the feed conversion ratio was 1.70±0.08. Compared with the third example group, the growth performance and intestinal health indicators all decreased to a certain extent (P<0.05). This clearly shows that adding nano-silica to the liposome coating layer can significantly improve the caking problem during feed processing and storage, maintain good fluidity and ingredient uniformity of the creep feed, and thus is beneficial to the piglets' feed intake and absorption of nutrients, having a positive impact on the growth performance and intestinal health of piglets.

[0153] In summary, components such as tannic acid, Macleaya cordata extract, chlorogenic acid, baicalin, glycerol butyrate and compound probiotics in the creep feed of the present invention jointly constitute a multi-target synergistic system of "antibacterial - anti-inflammatory - repair - regulation", and each component plays a synergistic role. By using the chitosan-liposome double-layer coating technology to coat tannic acid and adding nano-silica to the liposome coating layer, not only the antagonism problem between tannic acid and feed proteins and the caking problem of the feed are effectively solved, but also the effective replacement of the zinc oxide-containing formula is realized by antibacterial inhibition of harmful bacteria, anti-inflammatory relief of stress damage, repair of intestinal mucosa tissue and regulation of intestinal flora balance, comprehensively improving the intestinal function of piglets, significantly enhancing the growth performance of piglets, reducing the diarrhea rate, and providing a strong guarantee for the healthy growth of piglets.

[0154] The above are only the preferred embodiments of the present invention and do not impose any form of limitation on the present invention. Although the present invention has been disclosed above with the preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent embodiments by using the disclosed technical content within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A creep feed for suckling pigs for improving intestinal function, characterized in that, Comprising the following components in parts by weight: Among them, the coated tannic acid is coated with a chitosan solution.

2. The creep feed for sucking pigs for improving intestinal function according to claim 1, wherein The coated tannic acid has a double-layer coating structure. The inner layer of the coated tannic acid is chitosan, and the outer layer of the coated tannic acid is coated with a liposome suspension. The liposome suspension is prepared by dissolving soybean phospholipid and cholesterol in a mass ratio of (2-3):1 in phosphate buffer solution.

3. The piglet creep feed for improving intestinal function according to claim 2, wherein Nanosilica is added to the outer layer of the coated tannic acid.

4. The suckling pig creep feed for improving intestinal function according to claim 1, wherein, The creep feed also includes 0.05-0.1 part by weight of an antioxidant, and the antioxidant is selected from natural plant extracts. Preferably, the antioxidant is rosemary extract.

5. The suckling pig creep feed for improving intestinal function according to claim 1, wherein The creep feed also includes 0.5-1.0 part by weight of a compound probiotic, and the compound probiotic includes Lactobacillus plantarum, Enterococcus faecalis and Bacillus subtilis.

6. The creep feed for suckling pigs for improving intestinal function according to claim 1, wherein The creep feed also includes 0.9-1.5 parts by weight of glycine chelated trace elements, and the glycine chelated trace elements include zinc glycinate, iron glycinate and copper glycinate. Preferably, the weight part of zinc glycinate is 0.5-0.8 part, the weight part of iron glycinate is 0.3-0.5 part, and the weight part of copper glycinate is 0.1-0.2 part.

7. The creep feed for suckling pigs for improving intestinal function according to claim 1, characterized in that: The creep feed also includes 0.5-1.0 part by weight of a compound vitamin, and the compound vitamin includes vitamin A, vitamin D3, vitamin E and vitamin C.

8. The suckling pig creep feed for improving intestinal function according to claim 1, characterized in that: The protein-containing basic component comprises the following components in parts by weight:

9. The piglet creep feed for improving intestinal function according to claim 1, wherein The lipid-containing basic component is a fat powder.

10. The preparation method of the creep feed for suckling pigs for improving intestinal function according to any one of claims 1-9, characterized in that, Including the following steps: Z1. Raw material pretreatment: sufficiently pulverize the protein-containing basic component and the lipid-containing basic component to obtain a basic material; Z2. Mixing: fully mix the basic material with coated tannic acid, macleaya cordata extract, glucose oxidase, baicalin, chlorogenic acid, glycerol butyrate and benzoic acid to obtain a mixture; Z3. Granulation: granulate the mixture.

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