Compound feed additive for improving immunity of yellow feather broilers and preparation method of compound feed additive

Abstract

The invention provides a compound feed additive for improving the immunity of yellow feather broilers and a preparation method thereof, and relates to the technical field of broiler breeding feed preparation. The compound feed additive is prepared by mixing a macleaya cordata extract, an achyranthes bidentata root extract, a lotus root fermentation extract, chitosan oligosaccharide, fumaric acid, multivitamins and corn starch, wherein the lotus root fermentation extract is prepared by fermenting lotus roots with phaffia rhodozyma, squeezing, filtering, sterilizing and freeze-drying; the radix achyranthis bidentatae extract is prepared by performing microwave drying on radix achyranthis bidentatae, performing batch extraction by adopting 50%, 60% and 75% ethanol solutions, and performing drying. The method overcomes the defects in the prior art, effectively improves the growth efficiency of the yellow-feather chickens, further improves the immune performance of the yellow-feather chickens, and improves the economic benefits of breeding.

Description

Technical Field

[0001] The invention relates to the technical field of broiler breeding feed preparation, and in particular to a composite feed additive for improving the immunity of yellow-feathered broilers and a preparation method thereof. Background Art

[0002] In recent years, my country's chicken production and sales have shown a rapid upward trend. Yellow-feathered broilers are a broiler breed independently bred in my country. Compared with white-feathered broilers, the scale of my country's yellow-feathered broiler industry has generally shown a continuous upward trend, and the number of yellow-feathered broilers sold is almost the same as that of white-feathered broilers. With the continuous improvement of the intensive and large-scale breeding of yellow-feathered broilers, the intestinal health problems of yellow-feathered broilers have become increasingly prominent, and the incidence of intestinal diseases remains high, causing serious economic losses to breeders.

[0003] Since antibiotics have been widely used in the prevention, treatment and growth promotion of livestock and poultry diseases, there is still considerable controversy in the use of antibiotics to promote poultry growth. Antibiotics as feed additives have been banned by many countries. After the ban on antibiotics, some potential substitutes such as acidifiers, antimicrobial peptides, enzyme preparations and Chinese herbal extracts have been continuously developed as feed additives for broiler farming. In view of this situation, the use of safer plant extracts as feed additives is an important research direction at this stage. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a compound feed additive for improving the immunity of yellow-feathered broiler chickens and a preparation method thereof, which can further improve the immune performance of yellow-feathered chickens while effectively improving the growth efficiency of yellow-feathered chickens, thereby improving the economic benefits of breeding.

[0005] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solutions:

[0006] A composite feed additive for improving the immunity of yellow-feathered broilers, the composite feed additive being composed of the following materials in parts by weight: 40-60 parts of Macleaya cordata extract, 20-30 parts of Achyranthes bidentata root extract, 25-40 parts of lotus root fermentation extract, 8-12 parts of chitosan oligosaccharide, 2-6 parts of fumaric acid, 12-16 parts of complex vitamins, and 100-120 parts of corn starch; the Macleaya cordata extract is obtained by extracting Macleaya cordata by using acidic ethanol and ultrasound in combination; the Achyranthes bidentata root extract is obtained by drying the Achyranthes bidentata root by microwave, extracting in batches with 50%, 60%, and 75% ethanol solutions, and then drying; the lotus root fermentation extract is obtained by fermenting the lotus root with Phaffia rhodozyma, squeezing, filtering, and then sterilizing and freeze-drying.

[0007] Preferably, the preparation method of the Macleaya cordata extract comprises the following steps:

[0008] S1-1, adding 5-6 times the volume of 80% ethanol solution to Macleaya cordata, grinding into slurry, and then adjusting the pH to 5.0 to obtain pre-treated slurry for standby use;

[0009] S1-2, the pretreated slurry was heated to 60±5°C, ultrasonically treated at 600W for 20min, allowed to stand for 20min, ultrasonically treated again for 20min, filtered, and the filtrate was rotary evaporated and dried to obtain a Macleaya cordata extract.

[0010] Preferably, the method for preparing the lotus root fermentation extract comprises the following steps:

[0011] S1-1, wash the fresh lotus root, add 1-3 times the volume of distilled water, grind into pulp, add sucrose, stir evenly, and obtain composite pulp for later use;

[0012] S1-2, inoculating 0.1%-0.2% of the total mass of fresh lotus root with Phaffia rhodozyma into the composite slurry, fermenting for 3-5 days, and obtaining a fermented material for later use;

[0013] S1-3, the fermentation material is squeezed and filtered, 2-3 times the volume of distilled water is added to the filter residue, ultrasonically vibrated at 400W power for 5-10min, and then squeezed and filtered, and the two filtrates are combined to obtain the fermentation liquid for standby use;

[0014] S1-4, treating the fermentation liquid at 119° C. and 0.4-0.6 MPa for 10-15 min, concentrating it in vacuo to 1 / 5-1 / 3 of its original volume, and freeze-drying it to obtain lotus root fermentation extract.

[0015] Preferably, the amount of sucrose added in step S1-1 is 2%-3% of the mass of the fresh lotus root.

[0016] Preferably, the viable count of Phaffia rhodozyma in step S1-2 is 1×10 9 -2×10 10 Pieces / g.

[0017] Preferably, the preparation method of the Achyranthes bidentata root extract comprises the following steps:

[0018] S2-1, slicing the root of Achyranthes bidentata and drying it by microwave until the water content is ≤5%, and then crushing it to obtain Achyranthes bidentata root powder for later use;

[0019] S2-2, add 5-8 times volume of Achyranthes bidentata root powder to 50% ethanol solution, heat and stir in a water bath at 45±2°C for 1.5-2h, and then filter to separate and obtain a primary filtrate and a primary filter residue;

[0020] S2-3, add 5-8 times volume of 60% ethanol solution to the above primary filter residue, heat and stir in a water bath at 45±2℃ for 1.5-2h, and then filter to obtain secondary filtrate and secondary filter residue;

[0021] S2-4. Add 5-8 times volume of 75% ethanol solution to the above secondary filter residue, keep warm in a water bath at 45±2℃ with stirring for 1.5-2h, filter again, combine the filtrate with the above secondary filtrate and primary filtrate, remove the solvent by vacuum distillation, and then dry to obtain the Achyranthes bidentata root extract.

[0022] Preferably, the vitamin complex is obtained by mixing vitamin B6, vitamin B9, vitamin E and vitamin C in a mass ratio of 3:2:1:1.

[0023] The preparation method of the compound feed additive comprises the following steps:

[0024] (1) Add corn starch to 4-6 times the volume of clean water and stir to dissolve, then heat up for semi-gelatinization to obtain semi-gelatinized starch material for use:

[0025] (2) adding the Macleaya cordata extract, the Achyranthes bidentata root extract, the lotus root fermentation extract, fumaric acid, and multivitamins to the semi-gelatinized starch material, stirring evenly to obtain a mixed material for later use;

[0026] (3) Add chitosan oligosaccharide to the above mixture and continue to stir evenly, then dry and crush to obtain a composite feed additive.

[0027] The method of heating and semi-gelatinization treatment in step (1) is to control the temperature at 65±2°C and stir for 30-40 minutes.

[0028] Preferably, the drying method in step (3) is hot air drying at 30-35°C, and the powder is sieved through a 20-mesh sieve after being crushed.

[0029] The present invention provides a composite feed additive for improving the immunity of yellow-feathered broilers and a preparation method thereof, which has the advantages of:

[0030] The present invention uses Macleaya cordata extract, Achyranthes bidentata root extract, lotus root fermentation extract, chitosan oligosaccharide and fumaric acid as a feed additive. When added at 0.1% in the feed, the weight gain rate of yellow-feathered chickens can be effectively improved, and the immunity of yellow-feathered chickens can be improved. In addition, the addition of lotus root fermentation extract can improve the growth efficiency of yellow-feathered chickens while reducing the feed-to-weight ratio, improving the conversion rate of feed, and comprehensively improving the economic benefits of yellow-feathered chicken breeding. DETAILED DESCRIPTION

[0031] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The red Phaffia yeast used in the following examples was purchased from Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, with a deposit number of CGMCC NO.26861 and a viable cell count of 2×10 10 Saccharomyces boulardii was purchased from Sinopeptide Biotechnology (Shanxi) Co., Ltd. with the catalog number ZTSW-1113-271 and the viable count was 2×10 10 / g; the complex vitamin is obtained by mixing vitamin B6, vitamin B9, vitamin E and vitamin C in a mass ratio of 3:2:1:1.

[0033] Embodiment 1:

[0034] 1. Raw material preparation:

[0035] 1. Preparation of Macleaya cordata extract:

[0036] (1) adding 5 times the volume of 80% ethanol solution to Macleaya cordata, grinding into slurry, and then adjusting the pH to 5.0 to obtain a pretreated slurry for later use;

[0037] (2) The pretreated slurry was heated to 60±5° C., subjected to ultrasonic treatment at 600 W for 20 min, allowed to stand for 20 min, subjected to ultrasonic treatment again for 20 min, filtered, and the filtrate was rotary evaporated and dried to obtain a Macleaya cordata extract.

[0038] 2. Preparation of Achyranthes bidentata root extract:

[0039] 2.1. Achyranthes bidentata root extract A:

[0040] (1) Slicing the root of Achyranthes bidentata and drying it by microwave until the water content is ≤5%, and then crushing it to obtain Achyranthes bidentata root powder for later use;

[0041] (2) adding 6 times the volume of 50% ethanol solution to the powdered Achyranthes bidentata root, stirring and treating in a water bath at 45±2°C for 2 h, and then filtering to separate a primary filtrate and a primary filter residue;

[0042] (3) Add 6 volumes of 60% ethanol solution to the primary filter residue, heat and stir in a water bath at 45±2°C for 2 h, and then filter to obtain a secondary filtrate and a secondary filter residue;

[0043] (4) Add 6 times the volume of 75% ethanol solution to the secondary filter residue, heat and stir in a water bath at 45±2°C for 2 h, filter again, combine the filtrate with the secondary filtrate and the primary filtrate, remove the solvent by vacuum distillation, and then dry to obtain Achyranthes bidentata root extract A.

[0044] 2.2. Achyranthes bidentata root extract B

[0045] (1) Slicing the root of Achyranthes bidentata and drying it by microwave until the water content is ≤5%, and then crushing it to obtain Achyranthes bidentata root powder for later use;

[0046] (2) adding 6 times the volume of 75% ethanol solution to the powdered Achyranthes bidentata root, stirring and treating in a water bath at 45±2°C for 2 h, and then filtering to separate a primary filtrate and a primary residue;

[0047] (3) Add 6 volumes of 75% ethanol solution to the primary filter residue, heat and stir in a water bath at 45±2°C for 2 h, and then filter to obtain a secondary filtrate and a secondary filter residue;

[0048] (4) Add 6 times the volume of 75% ethanol solution to the secondary filter residue, heat and stir in a water bath at 45±2°C for 2 h, filter again, combine the filtrate with the secondary filtrate and the primary filtrate, remove the solvent by vacuum distillation, and then dry to obtain Achyranthes bidentata root extract B.

[0049] 3. Preparation of lotus root fermented extract:

[0050] 3.1. Lotus root fermented extract A

[0051] (1) Wash the fresh lotus root, add 2 times the volume of distilled water, grind into slurry, then add 2.5% sucrose of the total weight of the fresh lotus root, stir evenly, and obtain a composite slurry for later use;

[0052] (2) inoculating 0.15% of the total weight of fresh lotus root with Phaffia rhodozyma into the composite slurry, fermenting for 4 days, and obtaining a fermented material for later use;

[0053] (3) The fermented material was squeezed and filtered, and the residue was added with 2 times the volume of distilled water and ultrasonically vibrated at 400 W for 8 min, and then squeezed and filtered, and the two filtrates were combined to obtain the fermented liquid for later use;

[0054] (4) The fermentation liquid was placed at 119° C. and 0.5 MPa for 15 min, then concentrated under vacuum to 1 / 3 of the original volume, and then freeze-dried to obtain lotus root fermentation extract A.

[0055] 3.2 Lotus root fermented extract B

[0056] (1) Wash the fresh lotus root, add 2 times the volume of distilled water, grind into slurry, then add 2.5% sucrose of the total weight of the fresh lotus root, stir evenly, and obtain a composite slurry for later use;

[0057] (2) inoculating 0.15% of the total weight of fresh lotus root with Saccharomyces boulardii into the composite slurry, fermenting for 4 days, and obtaining a fermented material for later use;

[0058] (3) The fermented material was squeezed and filtered, and the residue was added with 2 times the volume of distilled water and ultrasonically vibrated at 400 W for 8 min, and then squeezed and filtered, and the two filtrates were combined to obtain the fermented liquid for later use;

[0059] (4) The fermentation liquid was placed at 119° C. and 0.5 MPa for 15 min, then vacuum concentrated to 1 / 3 of the original volume, and then freeze-dried to obtain lotus root fermentation extract B.

[0060] 3.3 Lotus root fermented extract C

[0061] (1) Wash the fresh lotus root, add 2 times the volume of distilled water, grind into slurry, then add 2.5% sucrose of the total weight of the fresh lotus root, stir evenly, and obtain a composite slurry for later use;

[0062] (2) inoculating 0.15% of the total weight of fresh lotus root with Phaffia rhodozyma into the composite slurry, fermenting for 4 days, and obtaining a fermented material for later use;

[0063] (3) The fermented material was squeezed and filtered, and the residue was added with 2 times the volume of distilled water and ultrasonically vibrated at 400 W for 8 min, and then squeezed and filtered, and the two filtrates were combined to obtain the fermented liquid for later use;

[0064] (4) The fermentation liquid was concentrated under vacuum to 1 / 3 of its original volume, and then freeze-dried to obtain lotus root fermentation extract C.

[0065] 2. Preparation of compound feed additives:

[0066] (1) Add corn starch to 5 times the volume of clean water and stir to dissolve, then heat to 65±2°C and stir for 35 minutes to perform semi-gelatinization treatment, and obtain semi-gelatinized starch material for use:

[0067] (2) adding the Macleaya cordata extract, the Achyranthes bidentata root extract, the lotus root fermentation extract, fumaric acid, and multivitamins to the semi-gelatinized starch material, stirring evenly to obtain a mixed material for later use;

[0068] (3) Add chitosan oligosaccharide to the above mixture and continue to stir evenly, then dry with hot air at 35°C and grind through a 20-mesh sieve to obtain a composite feed additive.

[0069] Referring to the above preparation process, different compound feed additives were prepared according to the weight ratio of each raw material and the raw material selection in Table 1 below:

[0070] Table 1

[0071]

[0072]

[0073] Embodiment 2:

[0074] Feeding experiment:

[0075] 700 newly hatched yellow-feathered broilers (hens) with an average body weight of 30g were selected and randomly divided into 7 groups, 1 blank group and test groups 1-6 for feeding experiments. The test period was 56 days. Each group had 5 replicates, and each replicate had 20 yellow-feathered broilers. The blank group was fed a basal diet; the test groups 1-6 were fed with 0.1% additives added to the basal diet. The feed intake and body weight on the 28th and 56th days were recorded for each replicate group, and the average daily weight gain (g / d), average daily feed intake (g / d) and feed-to-weight ratio of each stage of the experiment were calculated.

[0076] The composition of the basic diet is shown in Table 2 below:

[0077] Table 2

[0078]

[0079]

[0080] The nutritional composition of the premix is: manganese 11000 mg / kg, zinc 9000 mg / kg, iron 12000 mg / kg, copper 800 mg / kg, iodine 55 mg / kg, selenium 26 mg / kg, vitamin A 1400000 IU / kg, vitamin D3 280000 IU / kg, vitamin E 2380 mg / kg, vitamin K 196 mg / kg, vitamin B12 24 mg / kg, vitamin B2 840 mg / kg, vitamin B6 476 mg / kg, D-pantothenic acid 1120.00 mg / kg, niacin 4480.00 mg / kg, folic acid 112 mg / kg, biotin 5.6 mg / kg, vitamin B12 2.24 mg / kg.

[0081] The feed selections for each experimental group and blank group are as shown in Table 3:

[0082] Table 3

[0083] Group feed Blank group Basic diet Experimental Group 1 Basic diet + 0.1% additive 1 Experimental Group 2 Basic diet + 0.1% additive 2 Experimental Group 3 Basic diet + 0.1% additive 3 Experimental Group 4 Basic diet + 0.1% additive 4 Experimental Group 5 Basic diet + 0.1% additive 5 Experimental Group 6 Basic diet + 0.1% additive 6

[0084] On the 28th and 56th days of experimental feeding, one yellow-feathered broiler was selected from each treatment group and each replicate for weighing. After bleeding from the jugular vein, the abdominal cavity was opened, and the immune organs (thymus, spleen, bursa of Fabricius) were aseptically removed and weighed, and then the index of each immune organ was calculated.

[0085] On the 28th and 56th days of experimental feeding, one chicken was randomly selected from each replicate, and about 5 mL of blood was collected from the wing vein using a heparin anticoagulation vacuum tube. 2 ml of heparin anticoagulated blood (containing 20 U / mL) was fully mixed and allowed to stand at 37°C for 1 hour. After the red blood cells were completely precipitated, the plasma layer (with a little red blood cells) was taken with a pipette, and red blood cell lysis solution was added to lyse the red blood cells. The mixture was centrifuged at 3000 r / min at room temperature for 5 minutes, and the supernatant was discarded. The precipitate after centrifugation was washed twice with Han's solution, and the supernatant was discarded by centrifugation. Then the cells were resuspended in 2 ml of complete RPMI 1640 culture medium, and counted with trypan blue staining. The viable cells were above 95%, and the cell concentration was adjusted to 2×106 / mL to prepare a lymphocyte suspension.

[0086] Use a 48-well cell culture plate for culture. Add 100uL of lymphocyte suspension and 100uL of PHA solution to each test well to make the final PHA concentration in each well 25ug / mL. Add 100uL of lymphocyte suspension and 100uL of RPMI 1640 to each control well to make the final cell concentration 1×106. Add 200uL of RPMI 1640 to each blank well.

[0087] Each treatment was repeated three times and the cells were kept at 37°C and 5% CO 2 The cells were cultured in a cell culture incubator for 48 h. 4 h before terminating the culture, 20 uL of 5 mg / mL tetrazolium salt (MTT) solution was added to each well and incubated for another 4 h before terminating the culture.

[0088] Aspirate the culture supernatant in the wells, add 150uL dimethyl sulfoxide (DMSO) to each well, and shake for 10 minutes in the dark to fully dissolve the MTT. Measure the OD570 value on the microplate reader. The SI (stimulation index) value reflects the level of lymphocyte transformation rate. The SI calculation formula is:

[0089] SI = mean OD of PHA-stimulated wells / mean OD of control wells.

[0090] The specific test results of the above tests are shown in Table 4-7 below:

[0091] Table 4: Growth test of yellow-feathered chickens in each group at 28 days of age

[0092]

[0093]

[0094] Table 5: Growth test of yellow-feathered chickens in each group at 56 days of age

[0095] Group Weight (g) Daily weight gain (g) Feed intake (g) Material weight ratio Blank group 1911.28 33.59 83.59 2.49 Experimental Group 1 2243.45 39.53 84.57 2.14 Experimental Group 2 2250.92 39.66 84.72 2.14 Experimental Group 3 2167.11 38.16 84.65 2.22 Experimental Group 4 2173.27 38.27 84.59 2.21 Experimental Group 5 2078.52 36.58 84.22 2.30 Experimental Group 6 2014.35 35.43 84.69 2.39

[0096] Table 6: Immunity test of yellow-feathered chickens in each group at 28 days of age

[0097]

[0098] Table 7: Immunity test of yellow-feathered chickens in each group at 56 days of age

[0099]

[0100]

[0101] From the above test results, it can be seen that experimental groups 1-2 can effectively promote the growth of yellow-feathered chickens, enhance the immunity of yellow-feathered chickens, and the corresponding feed-to-weight ratio is lower, thereby improving the economic benefits of yellow-feathered chicken farming.

[0102] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A compound feed additive for improving the immunity of yellow-feathered broiler chickens, characterized in that: The compound feed additive is composed of the following materials in parts by weight: 40-60 parts of Macleaya cordata extract, 20-30 parts of Achyranthes bidentata root extract, 25-40 parts of lotus root fermentation extract, 8-12 parts of chitosan oligosaccharide, 2-6 parts of fumaric acid, 12-16 parts of complex vitamins, and 100-120 parts of corn starch; The Macleaya cordata extract is obtained by extracting Macleaya cordata by using acidic ethanol and ultrasound; The Achyranthes bidentata root extract is prepared by drying the Achyranthes bidentata root in microwaves, extracting it in batches using 50%, 60%, and 75% ethanol solutions, and then drying it; The lotus root fermentation extract is prepared by subjecting the lotus root to fermentation with Phaffia rhodozyma, followed by squeezing, filtration, sterilization and freeze-drying.

2. The compound feed additive according to claim 1, characterized in that The preparation method of the Macleaya cordata extract comprises the following steps: S1-1, adding 5-6 times the volume of 80% ethanol solution to Macleaya cordata, grinding into slurry, and then adjusting the pH to 5.0 to obtain pre-treated slurry for standby use; S1-2, the pretreated slurry was heated to 60±5°C, ultrasonically treated at 600W for 20min, allowed to stand for 20min, ultrasonically treated again for 20min, filtered, and the filtrate was rotary evaporated and dried to obtain a Macleaya cordata extract.

3. The compound feed additive according to claim 1, characterized in that The preparation method of the lotus root fermentation extract comprises the following steps: S1-1, wash the fresh lotus root, add 1-3 times the volume of distilled water, grind into pulp, add sucrose, stir evenly, and obtain composite pulp for later use; S1-2, inoculating 0.1%-0.2% of the total mass of fresh lotus root with Phaffia rhodozyma into the composite slurry, fermenting for 3-5 days, and obtaining a fermented material for later use; S1-3, the fermentation material is squeezed and filtered, 2-3 times the volume of distilled water is added to the filter residue, ultrasonically vibrated at 400W power for 5-10min, and then squeezed and filtered, and the two filtrates are combined to obtain the fermentation liquid for standby use; S1-4, treating the fermentation liquid at 119° C. and 0.4-0.6 MPa for 10-15 min, concentrating it in vacuo to 1 / 5-1 / 3 of its original volume, and freeze-drying it to obtain lotus root fermentation extract.

4. The compound feed additive according to claim 2, characterized in that: The amount of sucrose added in step S1-1 is 2%-3% of the mass of the fresh lotus root.

5. The compound feed additive according to claim 2, characterized in that: The number of viable bacteria of Phaffia rhodozyma in step S1-2 is 1×10 9 -2×10 10 Pieces / g.

6. The compound feed additive according to claim 1, characterized in that The preparation method of the Achyranthes bidentata root extract comprises the following steps: S2-1, slicing the root of Achyranthes bidentata and drying it by microwave until the water content is ≤5%, and then crushing it to obtain Achyranthes bidentata root powder for later use; S2-2, add 5-8 times volume of Achyranthes bidentata root powder to 50% ethanol solution, heat and stir in a water bath at 45±2°C for 1.5-2h, and then filter to separate and obtain a primary filtrate and a primary filter residue; S2-3, add 5-8 times volume of 60% ethanol solution to the above primary filter residue, heat and stir in a water bath at 45±2℃ for 1.5-2h, and then filter to obtain secondary filtrate and secondary filter residue; S2-4. Add 5-8 times volume of 75% ethanol solution to the above secondary filter residue, keep warm in a water bath at 45±2℃ with stirring for 1.5-2h, filter again, combine the filtrate with the above secondary filtrate and primary filtrate, remove the solvent by vacuum distillation, and then dry to obtain the Achyranthes bidentata root extract.

7. The compound feed additive according to claim 1, characterized in that: The complex vitamin is obtained by mixing vitamin B6, vitamin B9, vitamin E and vitamin C in a mass ratio of 3:2:1:

1.

8. A method for preparing the compound feed additive according to any one of claims 1 to 7, characterized in that: The preparation method comprises the following steps: (1) Add corn starch to 4-6 times the volume of clean water and stir to dissolve, then heat up for semi-gelatinization to obtain semi-gelatinized starch material for use: (2) adding the Macleaya cordata extract, the Achyranthes bidentata root extract, the lotus root fermentation extract, fumaric acid, and multivitamins to the semi-gelatinized starch material, stirring evenly to obtain a mixed material for later use; (3) Add chitosan oligosaccharide to the above mixture and continue to stir evenly, then dry and crush to obtain a composite feed additive.

9. The preparation method according to claim 8, characterized in that: The method of heating and semi-gelatinization treatment in step (1) is to control the temperature at 65±2°C and stir for 30-40 minutes.

10. The preparation method according to claim 8, characterized in that: The drying method in step (3) is hot air drying at 30-35°C, and the powder is sieved through a 20-mesh sieve after being crushed.

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