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Method for extracting 1-deoxynojirimycin from ramulus mori

A technology of deoxynojirimycin and mulberry branch, which is applied in the direction of organic chemistry, can solve the problems of polluting the environment and the value of resources has not been well developed, and achieves the effect of simple preparation process, easy operation and low cost

Inactive Publication Date: 2019-09-27
SUZHOU POLYTECHNIC INST OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the traditional sericulture production, a small part is used as fuel, and most of the mulberry branches are discarded, not only the resource value has not been well developed, but also polluted the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting 1-deoxynojirimycin from mulberry branches, comprising the following steps carried out in sequence:

[0025] (1) Mulberry branches (Hunan, Huanglutou old branches) were dried in a cool place (moisture content 10%), weighed 500kg of crushed and sieved powder (40 mesh), extracted 3 times with 70% ethanol-water solution at room temperature (4000L×1, 2500L×2, 12hr×3, solution circulation / stirring 10min every 2hr), combine 3 ethanol extracts, concentrate under vacuum at 55°C to 100L fluid.

[0026] (2) After degreasing the 100L fluid obtained in step (1) with petroleum ether, extract it three times with ethyl acetate (100L×3), combine the three ethyl acetate extracts, and recover the solvent under vacuum to obtain a fluid substance, 30 °C and dried under vacuum to obtain 4.7 kg of solid powder.

[0027] (3) Add 4.7 kg of the solid powder obtained in step (2) to 30% ethanol aqueous solution at 35°C to make a clear or slightly turbid (turbidity below 0....

Embodiment 2

[0030] A method for extracting 1-deoxynojirimycin from mulberry branches, which is basically the same as in Example 1, except that the elution in steps (3) and (4) is isocratic segmental elution, with 10% The concentration difference is an interval section, the elution volume of each section is 2 column volumes, and the eluent flow rate is 1 column volume / hour. Finally, 25 grams of 1-deoxynojirimycin crystals were obtained, and the content of 1-deoxynojirimycin was 95% as detected by HPLC.

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PUM

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Abstract

The invention relates to a method for extracting 1-deoxynojirimycin from ramulus mori. The method is characterized in that the ramulus mori is taken as a raw material and the method comprises the following steps: (1) extracting the ramulus mori by using an alcohol-water solution to obtain a crude extract; (2) performing ungrease treatment on the crude extract to obtain a semi-finished product; (3) dissolving the semi-finished product with an ethanol-water solution to obtain a loading solution with the turbidity lower than 0.5 degree, performing wet-process loading by adopting a macroporous resin D101 separation column, standing until resin reaches adsorption equilibrium, performing gradient elution or isocratic stepwise elution by taking the ethanol-water solution as an eluant, collecting eluant with the mass fraction of the 1-deoxynojirimycin greater than 20%, merging, concentrating, and drying to obtain a crude product; (4) repeating the purification in step (3) for the crude product, collecting eluant with the mass fraction of the 1-deoxynojirimycin greater than or equal to 95%, merging, concentrating, and drying to obtain the 1-deoxynojirimycin. The method can extract the 1-deoxynojirimycin efficiently from the ramulus mori, the operation is simple, the cost is low, and the method is suitable for mass production.

Description

technical field [0001] The invention discloses a method for extracting 1-deoxynojirimycin from mulberry branches. Background technique [0002] 1-Deoxynojirimycin is a glycosidase inhibitor with hypoglycemic, antiviral and antitumor metastasis effects. Studies have found that 1-deoxynojirimycin is contained in mulberry leaves, mulberry branches, mulberry and silkworm excrement. But mulberry leaves are an important raw material for silkworm rearing. The mulberry branch is the twig of the Moraceae plant mulberry. The mulberry branch accounts for about 64% of the annual dry matter of the mulberry garden, and it is the material with the highest proportion of biomass in the sericulture resources. However, in the traditional sericulture production, a small part is used as fuel, and most of the mulberry branches are discarded. Not only the value of its resources has not been well developed, but also the environment is polluted. [0003] It is known that mulberry branches contain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D211/46
CPCC07D211/46
Inventor 余雁司文会
Owner SUZHOU POLYTECHNIC INST OF AGRI
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