Preparation method of mangiferin

A mangiferin and crystallization technology, applied in the field of mangiferin preparation, can solve the problems of high cost, low content, unsatisfactory yield, etc., and achieve the effects of easy crystal formation and high purity

Inactive Publication Date: 2010-06-30
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of these methods have unsatisfactory yield, high cost and low content
Such as Chinese patent CN1203083C "Anemarrhena extract and its preparation method" and Chinese patent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Grind almond leaves to 30 meshes, take 10kg (mangiferin content 2.48%), add 120L saturated lime aqueous solution, heat to 50°C and soak for 2 hours, soak and extract twice, combine the extracts to adjust pH to 7 with hydrochloric acid, add column volume 5L of AB -8 macroporous resin column adsorption, first use 30L water to elute water-soluble impurities, then use 10L 30% ethanol to elute alcohol-soluble impurities, and finally use 25L 60% ethanol to elute mangiferin, the mangiferin eluate is concentrated to 20% alcohol concentration, and placed Crystallize to obtain coarse crystals of mangiferin, add the crude crystals of mangiferin to 60% methanol for reflux and dissolve, adjust the pH to 3 with hydrochloric acid, place the crystals, and finally obtain 206 g of finished mangiferin with a content of 96%.

Embodiment 2

[0026] Grind mango leaves to 50 meshes, take 10kg (mangiferin content 1.6%), add 100L saturated lime aqueous solution, heat to 60°C and soak for 1.5 hours, soak and extract twice, combine the extracts to adjust pH to 7.5 with sulfuric acid, add column volume 5L of AB -8 macroporous resin column adsorption, first use 40L water to elute water-soluble impurities, then use 15L 20% ethanol to elute alcohol-soluble impurities, and finally use 20L 70% ethanol to elute mangiferin, the mangiferin eluate is concentrated to 10% alcohol concentration, and placed Crystallize to obtain coarse crystals of mangiferin, add the crude crystals of mangiferin to 60% methanol for reflux to dissolve, adjust the pH to 4 with hydrochloric acid, place the crystals, and finally obtain 140 g of finished mangiferin with a content of 95%.

Embodiment 3

[0028] Almond leaves are crushed to 60 mesh, take 10kg (mangiferin content 2.2%), add 90L saturated lime aqueous solution and heat to 70°C and soak for 1 hour, soak and extract twice, combine the extracts to adjust pH to 8 with hydrochloric acid, add 5L column volume AB-8 macroporous resin column adsorption, first use 40L water to elute water-soluble impurities, then use 15L 10% ethanol to elute alcohol-soluble impurities, and finally use 25L 60% ethanol to elute mangiferin, and the mangiferin eluate is concentrated to an alcohol concentration of 20%. Stand to crystallize to obtain coarse crystals of mangiferin, add the crude crystals of mangiferin to 70% methanol for reflux to dissolve, adjust the pH to 4 with hydrochloric acid, place to crystallize, and finally obtain 192 g of finished mangiferin with a content of 95.5%.

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PUM

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Abstract

The invention relates to a preparation method of mangiferin. The preparation method adopts the process steps of saturated limewater solution soaking and extraction, macroporous resin adsorption, stepwise elution of water and ethanol solution of different concentration, concentration and crystallization under reduced pressure, and recrystallization by adjusting acidity through methanol. The obtained product has the advantages of high purity, high yield and low cost. The process is easy to popularize and industrialize.

Description

Technical field: [0001] The invention relates to a method for preparing mangiferin, in particular to a method for preparing mangiferin through processes such as alkaline water extraction, macroporous resin removal of impurities, acid adjustment and crystallization. Background technique: [0002] Mangiferin [0003] Molecular formula: C 19 h 18 o 11 [0004] Molecular weight: 422.33 [0005] Nature: also known as mango element, Zhimu Ning. Light grayish yellow needle crystal (50% ethanol). The melting point is 267-27°C. Optical rotation +43.3° (c=0.9, pyridine), 32° (ethanol). Slightly soluble in methanol, ethanol, water, soluble in hot dilute methanol, hot dilute ethanol, insoluble in non-polar solvents. [0006] Mangiferin has the functions of inhibiting the central nervous system, anti-inflammation, antibacterial, anti-herpes simplex virus, choleretic and immune, and has a protective effect on simulated plateau acute hypoxic liver injury. Mainly used in the treat...

Claims

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Application Information

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IPC IPC(8): C07D407/04
Inventor 刘东锋张翼杨成东
Owner NANJING ZELANG MEDICAL TECH
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