Method for separating and purifying (+)-isolariciresinol and (-)-lariciresinol from folium isatidis

Abstract

The invention relates to a method for separating and purifying (+)-isolariciresinol and (-)-lariciresinol from folium isatidis. The method comprises the following five steps of: (A) homogenate crushing enzymolysis: performing homogenate crushing of folium isatidis, and adding cellulase, pectinase or complex enzyme of the two for enzymolysis; (B) negative-pressure cavitation reinforced extraction: performing negative-pressure cavitation reinforced extraction of methanol, ethanol or alcohol-water mixed solution; (C) resin enrichment: loading a sample into a macroporous resin column, and performing stepwise elution and adsorption of macroporous resin column with target by use of 30-35% ethanol and 50-60% ethanol; (D) chromatographic separation: dissolving the sample with 90% methanol, performing gradient elution in a Toyopearl HW-40S gel resin column, and performing fractional collection of the fraction; and (E) crystallization: crystallizing a crude product of (+)-isolariciresinol and a crude product of (-)-lariciresinol with methanol to obtain the products of (+)-isolariciresinol and (-)-lariciresinol with purity over 95%. The method provided by the invention has the advantages of few steps, short time, little solvent consumption, high yield and relatively high economic value, and is more economical and environment-friendly and suitable for large-scale production.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a method for quickly and massively separating and purifying high-purity (+)-isolaricoresinol and (-)-laricine resin from Folium chinensis. Background technique [0002] Daqingye is a traditional Chinese medicine in my country, which is the leaf of Isatis indigotica Fort., a cruciferous plant. Bitter and cold in nature and flavor, Guixin and Stomach Meridian. It has the effects of clearing away heat and detoxification, cooling blood and eliminating spots, and is clinically used to treat infectious diseases such as various viral influenza, mumps and viral hepatitis (Zhong Weiguo et al., 2011). Pharmacological studies have shown that it has biological activities such as anti-virus, enhancing immunity, anti-endotoxin, anti-tumor, protecting liver and gallbladder, antibacterial, antipyretic, and anti-inflammatory (Zheng Xuehua, 2007). [0003] (+)-isolaricoresinol and (-)-laricine...

AI Technical Summary

Problems solved by technology

Although the activity of (+)-isolaricoresinol and (-)-lariforesin obtained from plants is outstanding, there are few reports on their isolation and purification (Liu Jifeng et al., 2006; Li Bin et al., 2005; Liu Haili et al. et al., 2002), and traditional purification techniques were used in these reports, which required organic solvent extraction and normal phase silica gel column chromatography. The process was long, the target compound was easy to lose, and the toxic organic reagents such as chloroform and dichloromethane A large amount of use is not conducive to environmental protection and health

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