Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of water-soluble brown natural pigment in camellia oleifera seed dregs

A technology of natural pigments and Camellia oleifera seed meal, which is applied in the direction of natural dyes, sugar derivatives preparation, chemical instruments and methods, etc., can solve the problems of waste, less utilization of camellia oleifera seeds, and discarded residues, and achieve the effect of solving the waste of resources

Inactive Publication Date: 2017-12-05
苏秀珍
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia oleifera seed meal is the residue left after oil-pressing of camellia oleifera seed. Due to the lack of research and understanding of camellia oleifera seed meal in the early stage, these residues after oil-pressing are usually discarded, causing great waste. With the deepening of research People found that in addition to the remaining vegetable oil, these residues also contain a considerable amount of useful nutrients and secondary metabolites such as tea saponin, tea polysaccharides, plant crude protein, and flavonoids.
Therefore, Camellia oleifera seed is currently mainly used to extract camellia oleifera seed oil, and the residual camellia oleifera seed meal is also continuously researched and used in aquaculture, biopesticides, feed, detergents, etc., as well as tea saponin, tea polysaccharides, etc. Biologically active substances, among which the extraction and application of tea saponin have been studied more, and the technology is relatively mature, but the utilization of other substances in Camellia oleifera seeds is relatively less, especially the pigment substances contained in it are usually destroyed during the operation process. Used as tea saponin or impurities in other products to remove

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of water-soluble brown natural pigment in camellia oleifera seed dregs
  • A kind of preparation method of water-soluble brown natural pigment in camellia oleifera seed dregs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Grind 50g of Camellia oleifera seed meal with a grinder to a particle size of 30 mesh, put it into a reflux extraction tank, add 125mL of 60% edible alcohol, heat and reflux for extraction for 2 hours, filter after cooling, and put the filter residue into the extraction tank repeatedly Add 125mL of 60% edible alcohol to extract for 2 hours, cool and filter, combine the filtrate, and concentrate the filtrate to dryness with a rotary evaporator at 45°C to obtain about 14g of extract.

[0027] (2) Take 50 g of polyamide filler (100-200 mesh), fill it in a glass chromatographic column with an inner diameter of 3 cm × 60 cm long, and equilibrate the chromatographic column with distilled water;

[0028] (3) Take 1 g of the sample obtained in step 1, dissolve it completely with a small amount of distilled water, load it on the polyamide chromatography column packed in step 2, wash it with 0.1% acetic acid solution at a flow rate of 25 ml / min, and use 500 ml as the volume T...

Embodiment 2

[0032] (1) Grind 100g of Camellia oleifera seed meal with a pulverizer to a particle size of 20 mesh, put it into an ultrasonic extractor and add 250mL of 95% edible alcohol, set the temperature at 45°C, and ultrasonically extract at an ultrasonic frequency of 40KHz for 1 hour, filter, and filter the residue Repeat the extraction twice under the same conditions in an ultrasonic extractor, filter, combine the filtrate, and concentrate the filtrate to dryness with a rotary evaporator at 45°C to obtain about 25 g of the extract.

[0033] (2) Take 60 g of polyamide filler (30-60 mesh), fill it in a glass chromatographic column with an inner diameter of 3 cm × 60 cm long, and equilibrate the chromatographic column with distilled water;

[0034] (3) Take 1 g of the sample obtained in step 1, dissolve it with a small amount of distilled water, and load it on the polyamide chromatography column packed in step 2

[0035] (4) Carry out elution with the mobile phase that contains edible al...

Embodiment 3

[0038] (1) Grind 100g of Camellia oleifera seed meal with a pulverizer to a particle size of 80 mesh, put it into an ultrasonic extractor and add 250mL of 95% edible alcohol, set the temperature at 45°C, and ultrasonically extract at an ultrasonic frequency of 40KHz for 1 hour, filter, and filter the residue Repeat the extraction twice under the same conditions in an ultrasonic extractor, filter, combine the filtrate, and concentrate the filtrate to dryness with a rotary evaporator at 45°C to obtain about 25 g of the extract.

[0039] (2) Take 55g of polyamide filler (60-90 mesh), fill it in a glass chromatographic column with an inner diameter of 3cm x 60cm long, and equilibrate the chromatographic column with distilled water;

[0040] (3) Take 1 g of the sample obtained in step 1, dissolve it with a small amount of distilled water, and load it on the polyamide chromatography column packed in step 2.

[0041] (4) Carry out elution with the mobile phase that contains edible al...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method of water-soluble tawny natural pigments in camellia cleifera seed meal. By taking residues after squeezing tea oil from camellia cleifera seed as a raw material, the method for preparing the high purity tawny pigments by the following processes: preparing raw materials of camellia cleifera seed meal; crushing by a crusher; extracting by edible alcohol; filtering; concentrating an extracting solution; carrying out polyamide column chromatography; carrying out stepwise elution on a mixture containing 0.1% acetate solution and an edible alcohol solution; and obtaining a tawny pigment product. The product comprises various flavone glycoside compounds, the main component comprises two compounds, and the purity of flavonoid pigment components in the final product detected by HPLC can reach over 70-95%. The obtained tawny pigments can be added into foods or cosmetics, particularly transparent liquid shampoo or shower gels which are clear and natural in color and have sense of beauty and natural antioxidant activity.

Description

technical field [0001] The invention relates to the extraction and preparation technology of a water-soluble brown natural pigment extracted from camellia oleifera seed dregs, and belongs to the technical field of food and medicine Background technique [0002] Camellia oleifera seed is the mature seed of Camellia oleifera Abel or C. meiocarpa Hu, ms., which is a unique edible vegetable oil resource in China. Camellia oleifera seed meal is the residue left after oil-pressing of camellia oleifera seed. Due to the lack of research and understanding of camellia oleifera seed meal in the early stage, these residues after oil-pressing are usually discarded, causing great waste. With the deepening of research It is found that in addition to the remaining vegetable oil, these residues also contain a considerable amount of useful nutrients and secondary metabolites such as tea saponin, tea polysaccharides, plant crude protein, and flavonoids. Therefore, Camellia oleifera seed is cu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08C09B61/00C09B67/54
CPCC07H1/08C07H17/07C09B61/00C09B67/0096
Inventor 陈全成石欣蕾张伟云苏秀珍洪珠凤
Owner 苏秀珍
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com