Preparation and purification method and applications of Toona sinensis fruit polysaccharide

Abstract

The invention discloses a preparation and purification method and applications of a Toona sinensis fruit polysaccharide, and relates to the technical field of extraction of Toona sinensis fruit polysaccharides. The preparation and purification method comprises: drying Toona sinensis fruits, crushing, leaching with hot water, sequentially carrying out rotary evaporation concentration, alcohol precipitation and freeze drying to obtain a crude polysaccharide, removing proteins with polyamide, decolorizing with active carbon, carrying out chromatography with a DEAE-Sepharose CL-6B anion exchange column, carrying out stepwise elution respectively with NaCl solutions with a concentration of 0, 0.1, 0.2 and 0.5 mol / L, collecting the eluent in the elution with the 0.2 mol / L NaCl, carrying out vacuum concentration, dialyzing with distilled water, and carrying out freeze drying to obtain the uniform Toona sinensis fruit polysaccharide STSP-3. According to the present invention, the prepared Toona sinensis fruit polysaccharide has high anticoagulant activity, the preparation method is simple, the potential safety hazards of the remaining chemical reagents are avoided, and the good developmentand application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of extracting polysaccharides from Toona sinensis, in particular to a method for preparing and purifying polysaccharides from Toona sinensis and its application. Background technique [0002] With the improvement of people's living standards, changes in eating habits and the acceleration of the pace of life, people pay more attention to their own health; extracting active ingredients from natural products and developing and utilizing them has achieved unprecedented development. And thrombotic disease seriously affects people's health, and there is an increasing trend in recent years. It is one of the important topics in the medical field. At present, the more effective drug for treating thrombotic disease is the polysaccharide drug heparin, but it can Lead to many adverse side effects, such as osteoporosis, continuous intravenous injection, thromboembolic complications, dependence on antithrombin and anticoa...

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Problems solved by technology

Chinese toon seed water decoction has strong antithrombotic effect [Jing Guilan, Chen Chao. Study on the antithrombotic effect of Chinese toon seed water decoction[J]. Chinese Pharmacy, 2011,22(15):1364-1366] Chinese toon seed water decoction The drug contains many water-soluble and hydrophilic chemical components, such as alkaloids, saponins, steroids, polysaccharides, proteins, water-soluble pigments, plant phenols, etc., and the main components of the drug effect are not clear; the crude polysaccharide of Toona sinensis has anticoagulant effect [Yan Wenxia. Study on anticoagulant effect of Toona sinensis polysaccharides [D]. China Three Gorges University, 2013]. Proteins and pigments are combined in the complex, and neutral polysaccharide components and acidic polysaccharide components are entangled and cross-linked, but specific Which polysaccharide in Toona sinensis polysaccharide produces the anticoagulant effect, so far there is still no clear research result

[0004] At the same time, the applicant found that the existing methods for extracting Chinese toon seed polysaccharides mostly prepared Chinese toon seed crude polysaccharide, which is a mixture, and some impurities, such as pigments and proteins, will be produced during the initial extraction. To a large extent, it will affect the appearance and purity of the polysaccharide, and the components are relatively complex, which increases the uncertainty of the research on the anticoagulant biological properties of the polysaccharide from Toona sinensis, thus limiting the market promotion of the biological activity of the polysaccharide from Toona sinensis

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