Preparation and purification method and applications of Toona sinensis fruit polysaccharide

A purification method and technology of Toona sinensis, applied in the field of preparation and purification of Toona sinensis polysaccharides, can solve the problems of restricting the market promotion of the biological activity of Toona sinensis polysaccharides, increasing the uncertainty of anticoagulant biological performance research, polysaccharide appearance, and the influence of purity , achieve good anticoagulant effect, prolong prothrombin time, and simple preparation method

Active Publication Date: 2019-11-19
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese toon seed water decoction has strong antithrombotic effect [Jing Guilan, Chen Chao. Study on the antithrombotic effect of Chinese toon seed water decoction[J]. Chinese Pharmacy, 2011,22(15):1364-1366] Chinese toon seed water decoction The drug contains many water-soluble and hydrophilic chemical components, such as alkaloids, saponins, steroids, polysaccharides, proteins, water-soluble pigments, plant phenols, etc., and the main components of the drug effect are not clear; the crude polysaccharide of Toona sinensis has anticoagulant effect [Yan Wenxia. Study on anticoagulant effect of Toona sinensis polysaccharides [D]. China Three Gorges University, 2013]. Proteins and pigments are combined in the complex, and neutral polysaccharide components and acidic polysaccharide components are entangled an...

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  • Preparation and purification method and applications of Toona sinensis fruit polysaccharide
  • Preparation and purification method and applications of Toona sinensis fruit polysaccharide
  • Preparation and purification method and applications of Toona sinensis fruit polysaccharide

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Embodiment 1

[0031] It can be understood that in the prior art, most of the extraction of Toona sinensis polysaccharides is to obtain the crude polysaccharides of Toona sinensis, because the crude polysaccharides contain impurities, such as pigment proteins, etc., which have a certain impact on the color and purity of the polysaccharides. , and lead to the inability to determine the bioactive components in the follow-up study of its biological activity, which is not conducive to the application and research of Toona sinensis polysaccharide.

[0032] In order to solve all or part of the deficiencies in the prior art, the present invention provides a Chinese toon polysaccharide, which is a monosaccharide with uniformity, and includes the following molar percentage components: arabinose 2% to 7%, rhamnose 20-30%, glucosamine 1.5-5.5%, galactose 45-60%, glucose 2.5-10%, xylose 0.25-1%, mannose 2.5-4%.

[0033] Simultaneously, the present invention provides a kind of preparation, purification m...

Embodiment 2

[0092] Embodiment 2 of the present invention provides the preparation of an anticoagulant health care product - Toona sinensis oral liquid. Specifically include the following steps:

[0093] In parts by weight, the ratio of water to Chinese toon seed coarse powder is 1-30:100, the extraction temperature is 65-90°C, and the time is 10-30min. After leaching, filter the slag with 250-mesh nylon cloth, cool rapidly, and place for 24h Clarification, 0.5 μm fine filtration to obtain a clarified sample liquid, that is, Chinese toon seed juice. Then 0.03-0.4% of STSP-3, 4-6% of sucrose, 0.03-0.07% of ascorbic acid and other auxiliary materials are dissolved in some warm water and filtered. Mix it with the above-mentioned Toon Seed Juice, then sterilize it at 135°C for 5-10 seconds, and then can prepare anticoagulant health-care Chinese Toon Seed Oral Liquid (set the volume of each bottle of oral liquid to be 8-10 mL).

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Abstract

The invention discloses a preparation and purification method and applications of a Toona sinensis fruit polysaccharide, and relates to the technical field of extraction of Toona sinensis fruit polysaccharides. The preparation and purification method comprises: drying Toona sinensis fruits, crushing, leaching with hot water, sequentially carrying out rotary evaporation concentration, alcohol precipitation and freeze drying to obtain a crude polysaccharide, removing proteins with polyamide, decolorizing with active carbon, carrying out chromatography with a DEAE-Sepharose CL-6B anion exchange column, carrying out stepwise elution respectively with NaCl solutions with a concentration of 0, 0.1, 0.2 and 0.5 mol/L, collecting the eluent in the elution with the 0.2 mol/L NaCl, carrying out vacuum concentration, dialyzing with distilled water, and carrying out freeze drying to obtain the uniform Toona sinensis fruit polysaccharide STSP-3. According to the present invention, the prepared Toona sinensis fruit polysaccharide has high anticoagulant activity, the preparation method is simple, the potential safety hazards of the remaining chemical reagents are avoided, and the good developmentand application prospects are achieved.

Description

technical field [0001] The invention relates to the technical field of extracting polysaccharides from Toona sinensis, in particular to a method for preparing and purifying polysaccharides from Toona sinensis and its application. Background technique [0002] With the improvement of people's living standards, changes in eating habits and the acceleration of the pace of life, people pay more attention to their own health; extracting active ingredients from natural products and developing and utilizing them has achieved unprecedented development. And thrombotic disease seriously affects people's health, and there is an increasing trend in recent years. It is one of the important topics in the medical field. At present, the more effective drug for treating thrombotic disease is the polysaccharide drug heparin, but it can Lead to many adverse side effects, such as osteoporosis, continuous intravenous injection, thromboembolic complications, dependence on antithrombin and anticoa...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/7004A61P7/02A23L33/125
CPCA23V2002/00A61K31/7004A23L33/125A61P7/02C08B37/0003C08B37/006A61K2300/00A23V2200/326A23V2250/602A23V2250/624A23V2250/308A23V2250/608A23V2250/61A23V2250/618A23V2250/638
Inventor 蔡为荣闻志莹程浩宇许永
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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