Separation and purification method for isomers of anthocyanin compound in lycium ruthenicum murr

A technology for separation and purification of Lycium barbarum, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of limited separation selectivity and insufficient separation capacity of reversed-phase chromatography, and achieve the effect of high-efficiency preparation

Inactive Publication Date: 2016-12-07
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, for cis-trans isomerized anthocyanins, the separation ability of reversed-phase chromatography is often insufficient, mainly due to limited separation selectivity

Method used

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  • Separation and purification method for isomers of anthocyanin compound in lycium ruthenicum murr
  • Separation and purification method for isomers of anthocyanin compound in lycium ruthenicum murr
  • Separation and purification method for isomers of anthocyanin compound in lycium ruthenicum murr

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Embodiment 1

[0021] 1) Lycium barbarum extract was separated by preparative reversed-phase high-performance liquid chromatography (RPLC). Chromatographic conditions: the chromatographic column was a carbon-octave column (XTerra MS C18 column); the flow of trifluoroacetic acid aqueous solution A and methanol B was Phase system, wherein, the volume concentration of trifluoroacetic acid aqueous solution is 0.2%; the elution gradient is 0-40min, the volume concentration is 15% → 80% B; the flow rate is 80mL / min; the detection wavelength is 520nm; the sample solution concentration is 300mg / min mL, which is dissolved in 0.5% (v / v) formic acid aqueous solution; the injection volume is 5 mL; fractions are collected according to the chromatographic peaks, a total of 14 fractions are collected, and each fraction is concentrated to dryness for later use.

[0022] 2) For the two fractions rich in anthocyanin isomers, marked as Fraction 6 and Fraction 9, they were prepared by mixed mode chromatography, ...

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Abstract

The invention provides a separation and purification method for isomers of an anthocyanin compound in lycium ruthenicum murr. Particularly, the method is to efficiently separate and purify the isomers of the anthocyanin compound from a lycium ruthenicum murr extract by adopting mixed-mode chromatography; a mixed-mode chromatographic column is an inverted-phase / strong anionic exchange mixed-mode chromatographic column; efficient separation of the anthocyanin isomers is realized under a low-pH condition by adopting the mixed-mode chromatography; a linear gradient, stepwise gradient or isocratic elution mode is used. A lycium ruthenicum murr ethyl alcohol extract is selected for inversed phase chromatographic preparation and separation to obtain 2 fractions; two pairs of cis-trans anthocyanin isomers, including a new anthocyanin and 3 anthocyanin monomer compounds separated from the lycium ruthenicum murr at the first time, are prepared through the mixed-mode chromatography. The method is excellent in separation effect on the cis-trans anthocyanin isomers, and can realize efficient separation and purification of the anthocyanin isomers and provide a substance basis for research on the activity of an anthocyanin compound.

Description

technical field [0001] The invention relates to the separation and purification of anthocyanin compounds, in particular to a method for efficiently separating anthocyanin isomers under low pH conditions by using mixed-mode chromatography. This method showed a better separation effect on cis-trans anthocyanin isomers. Background technique [0002] Anthocyanins are a class of compounds formed by the combination of anthocyanins and sugars through glycosidic bonds. They are widely present in the cell fluids of flowers, fruits, stems, leaves and root organs of plants, making them appear in different colors from red, purple to blue. . Anthocyanins are flavonoids – a family of compounds that give red color to a class of substances based on flavonoid cores. According to literature reports, this type of compound has good biological activities, such as preventing cancer and heart disease, anti-inflammation and anti-oxidation (Hou, D.X. (2003). Potential mechanisms of cancer chemopre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/065C07H1/08
Inventor 梁鑫淼金红利刘艳芳郭志谋张秀莉薛兴亚
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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