Mannuronic acid and guluronic acid imbedded oligosaccharin and preparation method therefor

A technology of guluronic acid and mannuronic acid, applied in oligosaccharides and other directions, can solve problems such as weak glycosidic bonds, difficult to obtain, and influence research.

Inactive Publication Date: 2004-11-17
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because the glycosidic bond between mannuronic acid and guluronic acid is weak, it is difficult to obtain this substance, which naturally affects further research on it.

Method used

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  • Mannuronic acid and guluronic acid imbedded oligosaccharin and preparation method therefor
  • Mannuronic acid and guluronic acid imbedded oligosaccharin and preparation method therefor
  • Mannuronic acid and guluronic acid imbedded oligosaccharin and preparation method therefor

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Embodiment Construction

[0011] Prepare alginate into a 5% (weight percent concentration, the same below) aqueous solution, add alginate lyase (Vibro Sp.510) to react, heat in a boiling water bath to inactivate the enzyme, remove precipitated impurities after centrifugation, and The obtained supernatant was separated and purified by gel permeation chromatography, the packing material was Bio-Gel (Bio-Gel) P6 and the strong anion exchange chromatography packing material was Spherisorb SAX, and the non-reducing end had a double bond of mannuronic acid and ancient glucuronic acid mosaic fragment oligosaccharide; and introduce SO at the 2 and 3 positions of the sugar ring 3 Na or CH 3 or PO 3 Na 2 . Its structural formula is:

[0012]

[0013] or

[0014]

[0015] In the formula, n=1-19, m=1-19, n+m3 Na or CH 3 or PO 3 Na 2

[0016] The concentration of the alginate aqueous solution in the present invention can be 1-10%. The alginate lyase used is a specific alginate lyase. In addition to...

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Abstract

The invention relates to a mannuronic acid and guluronic acid imbedded oligosaccharin, characterized in that the non-deacidizing end has double bonds, the preparation comprises preparing algin into water solution, charging algin cracking enzyme for reaction, heating by boiling water bath, centrifuging to remove settled foreign substance, separating and purifying through chromatography.

Description

technical field [0001] The invention relates to a mosaic fragment oligosaccharide of mannuronic acid and guluronic acid with double bonds at the non-reducing end and a preparation method thereof. Background technique [0002] Alginate is a linear polysaccharide composed of β-mannuronic acid and α-guluronic acid as monomers linked by 1-4 glycosidic bonds. From the perspective of the whole molecule, alginate can be divided into three fragments: polymannuronic acid fragment (M block), polyguluronic acid fragment (G block) and mannuronic acid and guluronic acid mosaic fragment ( MG block). The ratio of mannuronic acid to guluronic acid in alginate varies according to different algae, different regions, different seasons and different distribution parts in algae, which is roughly 2:1 to 1:2. In recent years, the biological activities of algin oligosaccharides and their derivatives have been continuously discovered, ranging from anticoagulant, antibacterial, anti-inflammatory, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C08B37/00
Inventor 管华诗张真庆于广利江晓路
Owner OCEAN UNIV OF CHINA
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