Sleeve for biological sample in-vitro purification treatment

Abstract

The invention relates to a sleeve for biological sample in-vitro purification treatment. The sleeve comprises a pipe body, multiple movable baffles, a connecting shaft, a sealing ring and a coaxial locking switch, wherein the pipe body is cylindrical, a flange is arranged at the upper end of the pipe body, multiple first openings are formed in the end face of the bottom of the pipe body, and multiple second openings and convex blocks are arranged at the bottom end of the side wall of the pipe body; the sealing ring is sleeved on the outer side of the second openings; the movable baffles are arranged at the bottom of the pipe body, are of an annular fan shape and are provided with multiple third openings, and the outer edges of the third openings are in one-to-one correspondence with the second openings and are contacted with the sealing ring; the connecting shaft is positioned at a central position of the pipe body, the top end of the connecting shaft is connected with the coaxial locking switch, a polygonal part is arranged at the bottom end of the connecting shaft, and the edges of the polygonal part are in one-to-one correspondence with the inner edges of the movable baffles; the coaxial locking switch is positioned on the upper surface of the flange of the pipe body and can rotate. The sleeve provided in the invention is a device which is convenient, practical, low in production cost, simple in production process, safe and non-toxic and is used for biological sample in-vitro treatment.

Description

technical field [0001] The invention relates to a sleeve used for in vitro purification treatment of biological samples, which belongs to the technical field of biomedicine. Background technique [0002] The in vitro enrichment of biological particles in biological samples by centrifugation is one of the most widely used techniques in biological research. Many biological particles can be enriched by centrifugation, such as animal or plant cells, subcellular structures, bacteria, viruses or biomacromolecules. The behavior of these biological particles in suspension under centrifugation mainly depends on the size and density of the biological particles, which can be explained by the Stokes equation. The Stokes equation (see the following formula) describes the changing law of the motion velocity of spherical particles when they are subjected to centrifugal action in a liquid medium: [0003] v = d 2 ...

AI Technical Summary

Problems solved by technology

The solution of pre-installed density media is indeed a good solution to the problem described in the above defect (a), but it also brings the following defects: (d) the storage method of this type of product must be designed separately for the density media Certainly

In summary, centrifuge tubes with a porous filter barrier structure pre-loaded with an appropriate density medium not only cannot solve the problems described in (b) and (c) in the above defects, but also introduce additional defects in (d) and (e) Issues of preservation, product safety and efficacy as described

[0009] Chinese patent 200610063125.4 provides a lymphocyte separation tube with a loose porous material at the bottom of the tube. The loose porous material can absorb the density medium into a semi-solid state. When the blood sample is purified, the blood sample can be directly poured into the tube. One kind of separation tube, without worrying about mixing the interface between the blood and the separation fluid, which solves the time-consuming and laborious problem of "spreading blood" operation

However, this kind of lymphocyte separation tube with a loose porous material at the bottom of the tube still faces the time-consuming problem of adding the density medium. Since the loose porous material can prevent blood from penetrating immediately, the adsorption and penetration of the density medium also takes a certain time. It needs to be achieved by "dropping"; and, because the porous porous material itself is not transparent, it is difficult to accurately control the amount of density medium added, which in turn brings great benefits to the control of the separation effect of blood samples and the reproducibility of results. very difficult

The above-mentioned patent also provides another product form, that is, the lymphocyte separation liquid (density medium) is added to the loose porous material of the tube body in advance, although this can realize "ready-to-use" and accurate control of the amount of density medium added, However, the material of loose porous materials also faces the problems of preservation, product safety and effectiveness described in the above defects (d) and (e)

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