Nucleic acid extraction method and nucleic acid extraction reagent for biological samples

A biological sample, nucleic acid technology, applied in the field of nucleic acid extraction, can solve the problems of long time and complicated operation steps, and achieve the effect of weakening the agglutination effect, increasing the pH value, and enhancing the hydrolysis ability and
CN105176969AActive Publication Date: 2015-12-23USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
USTAR BIOTECHNOLOGIES (HANGZHOU) CO LTD
Publication Date
2015-12-23

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Abstract

The present invention relates to the field of nucleic acid extraction, particularly to a nucleic acid extraction method and a nucleic acid extraction reagent for biological samples. The specific method comprises: adding a sodium hydroxide solution to a biological sample, adding a lysate, and carrying out lysis to obtain a lysis reaction liquid, wherein the lysate contains a guanidine salt, disodium edetate, a surfactant and a buffer agent; and adding an ethanol solution to the lysis reaction liquid, and achieving nucleic acid adsorption, cleaning and elution by using an instrument-free nucleic acid extraction apparatus having a silica gel membrane or a centrifugal column. According to the present invention, the sodium hydroxide is added to pre-treat and then the lysate is added to carry out lysis, such that the protein in the sample can be well dissolved, and the binding of the nucleic acid and the adsorption film is prompted; and the lysate component is optimized, such that the binding capacity of the nucleic acid molecule and the silica gel membrane under the high salt and high pH value conditions is significantly enhanced, and the good nucleic acid extraction efficiency is ensured.
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Description

technical field

[0001] The invention relates to the field of nucleic acid extraction, in particular to a nucleic acid extraction method and reagents for biological samples. Background technique

[0002] Nucleic acid extraction is the first step in many clinical tests. The concentration and purity of the nucleic acid templates obtained in this link directly affect the accuracy of subsequent test results. The spin column method is a common nucleic acid extraction method in clinical testing. This method utilizes the characteristic that nucleic acid molecules bind to silica gel membrane in high-salt and low-pH environment, and dissociate from silica gel membrane in low-salt and high-pH environment. Enrich and purify nucleic acid templates. Combined with a high-speed centrifuge or an automated nucleic acid extractor, the spin column method has the advantages of large throughput, fast speed, high extraction efficiency, and good template purity, which can meet the inspection req...

Claims

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