Red cell storage solution and preparation method thereof

Abstract

The invention relates to a red cell storage solution and a preparation method thereof. The storage solution comprises a buffering system, a nutritional system, an osmotic pressure system and an anticorrosion system, wherein the anticorrosion system comprises the following components according to mass concentration: 0.2-1g / L of chloramphenicol, 0.1-0.6g / L of neomycin sulfate, and 0.1-0.5g / L of gentamicin. Through mutual concurrent effects of the buffering system, the nutritional system, the anticorrosion system and the osmotic pressure system of the red cell storage solution provided by the invention, long storage cycle of the red cell storage solution is ensured, at the end of the sixth month of the storage, that red cell suspension free hemoglobin concentration still conforms to national standard is detected, and the hemolysis ratio at the end of the storage is smaller than 0.8 percent of the total of red cells; the red cell storage solution provided by the invention can ensure relatively strong antigenic activity and stability of the red cells.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the technical field of blood transfusion testing, in particular to a red blood cell preservation solution and a preparation method thereof. Background technique [0002] With the gradual increase of medical technology, that is, people's safety awareness, blood preservation before blood transfusion is more and more used in the treatment of some large diseases. Accurate blood type testing before blood transfusion is the premise of safe blood transfusion in medical treatment. Blood transfusion has healed countless lives as a treatment method, but blood transfusion may also lead to serious adverse reactions of blood transfusion, even life-threatening. Immediate hemolytic transfusion reactions are common in ABO incompatible blood transfusions, so the accurate determination of ABO blood type is very important. ABO blood group positive typing test is particularly prone to false positive or f...

AI Technical Summary

Problems solved by technology

[0005] At present, there is no unified maintenance system in China, and the red blood cell processing technology is not correspondingly perfect, which makes it difficult to guarantee the preservation of red blood cells in vitro

In foreign countries, Alfred's solution with a pH value of 6.8±0.2 is used as the red blood cell preservation solution, which is also more formal and standardized. However, considering the short storage time of the product, it is only 3 months, plus the long transportation cycle and the antigen on the red blood cell membrane during the process. Influenced by stability and activity, the effective period of use is shortened, and the cost increases

Images